Finding Organic Compounds Like Carbohydrates, Lipids, Proteins And Nucleic Acid

Finding Organic Compounds Like Carbohydrates, Lipids, Proteins And Nucleic Acid

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To uncover organic compounds like carbohydrates, lipids, proteins and nucleic acid, by using tests like Benedict, Lugol, Biuret and Beta Carotene. Each test was used to determine the presents of different organic molecules in substances. The substances that were tested for in each unknown sample were sugars, starches, fats, and oils. Moreover, carbohydrates are divided into two categories, simple and complex sugars. Additionally, for nonreducing sugars, according to Stanley R. Benedict, the bond is broken only by high heat to make make the molecules have a free aldehydes (Benedict). As for Lipids, there are two categories saturated and unsaturated fats. One of the difference is that saturated fats are mostly solids and have no double bond (Campbell Biology 73). The Beta Carotene test works by dissolving in a lipid, thus giving it color to make it visible. Moreover, proteins are made out of amino acids that are linked by a polypeptide bond (Campbell Biology 75). The purpose of this experiment was to determine whether an unknown class sample or food sample had any carbohydrates, lipids, or proteins in it. The expected result of the lab was that some substances would be present while other would be absent.
Materials and Methods :
The first test that was conducted was the Benedict test, which was used to test for simple, disaccharide, and non reducing sugars. A total of 2 test tubes were set as controls. The purpose of the control was to established what a positive sample and negative sample would eventually look like. For the negative test tube, 2 ml of water were added and 2 ml of glucose were added to the positive second test tube. 2 ml of Benedict’s solution were added to both test tube and heated in boiling water for 3 minutes....

... middle of paper ...

... Beta Carotene test come back positive for lipids.
As for the expected result, it was proposed that some of the tests would be positive while other would be negative. The expected result turned out to be correct as it was proven throughout the lab during the testing of the unknown solutions and food sample.
Different unknown solution A-C were positive for either starch, protein, lipids, or neither just like what was seen in the last unknown solution, D. When more research was done on organic compounds it should that the unknown solution was likely one of the unaccounted organic molecules, nucleic acid. Moreover, the Chex™ cereal square was positive for starch because its main ingredient is corn. Surprisingly, it also came back positive for lipids, but when more research was done on how it was processed, it made sense that it was positive for lipids.

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