The results verified that the spinach had the highest chloroplasts concentration because it had the absorbency of 0.730, 0.826, 1.011, and 1.049 as shown in Table 1-4. The spinach was our positive control because, from a previous experiment, we knew that it would have chloroplasts; the buffer sucrose was the negative control because if the cabbage or lettuce did not have any contents of chloroplasts, then the readings should be equivalent to the buffer sucrose since it was our blank solution. Therefore, it should read 0.00, if there were no contents of chloroplasts. On the other hand, there was a flux in the data between the cabbage and the lettuce. However, the overall data suggested that the cabbage had a higher content of chloroplasts than …show more content…
The chloroplasts within the spinach were triggered by the light in the room, as the function of chloroplasts is to induce photosynthesis. A similar experiment was tested by Clapper and Sallee. They concluded that the rate of photosynthesis of the spinach solution was greater than it was in both the cabbage and lettuce. Clapper and Sallee stated that the red cabbage and lettuce may have had slower growth rates, which was why the chloroplasts concentration plateaued after 5 minutes from the first reading. In Table 4, we tested the concentration of each plant at a wavelength of 500nm to see if we’d get a better reading. The same results showed that spinach had the highest concentration of chloroplasts and lettuce had the least concentration. In spite of it all, there has been debate over the accuracy of calibrating the chloroplasts concentration through a spectrophotometer because some scientists argued that low contents of chlorophyll a were more sensitive at a wavelength of 675nm compared to a wavelength at 550nm (Xue and Yang,
The hypothesis for this experiment was that the cell fraction in the cuvette marked P2 will have more chloroplast activity because it will exhibit greater color change and differences in the absorbance readings compared to the other cuvettes when exposed under the condition of light; moreover, this notion was believed to be so because the more a cell fraction is centrifuged, the more intact chloroplasts we’ll find (Leicht and McAllister,
ABSTRACT: Chloroplasts carry out photosynthetic processes to meet the metabolic demands of plant cells (Alberts, 2008). They consist of an inner thylakoid membrane and a stroma. (Parent et. al, 2008).In this experiment we demonstrate the unique protein compositions of isolated thylakoid and stromal fractions from broken and whole spinach chloroplasts. Because these compartments carry out different metabolic processes, we confirm our hypothesis that performing SDS-PAGE on these fractions will result in distinct patterns on the gels. In isolating and analyzing nucleic acid from broken, whole, and crude chloroplast samples we demonstrate that genes for photosynthetic protein psbA are found in chloroplast DNA, while genes for photosynthetic enzyme
... in the chloroplasts in some of their cells. Chlorophyll allows the energy in sunlight to drive chemical reactions. Chloroplasts act as energy transducers, converting light energy into chemical energy. So as the plant has more light the chlorophyll inside the chloroplasts can react faster absorbing in more light for food and energy.¡¨ So this shows my prediction was correct for in my experiment and shown in my result table and graph the more light intensity there is on a plant the higher the rate of my photosynthesis will be. My prediction is very close to what I said the results will be so my prediction was correct and has been proven to be correct in my result table, graph and now explained again in my conclusion.
It can be concluded that chloroplast is responsible for photosynthesis, with blue light forming the highest rate of photosynthetic activity. The widespread use of coloured netting in the future could result in indoor plant growth that is unreliant on weather, and the ease of the production of crops with the desirable phenotypes. However, future research is still required to eradicate any unknown data and determine plant responses in relation to wavelength
Experiment #1: The purpose of this experiment is to investigate the effects of baking soda and light intensity on the rate of photosynthesis of green spinach leave through the observation of floating disk.
= > [CH2O} + O2 + H2O, This shows that when the light intensity is increased the rate of reaction will be more quicker he only anomalous result there was, is the one in the 100 watt result the reading after 5 minutes is anomalous because it does not follow the predicted pattern of increasing in the production of gas because it is lower I know from my own knowledge of photosynthesise that when the light intensity is increased the rate of reaction will be more quicker because many plants and trees photosynthesise quicker in stronger light and photosynthesise slower in dimly lit places. The chlorophyll absorbs light energy and enables it to be used by the plant for building up sugar. The overall effect is that energy is transferred from sunlight to sugar molecules.
Moreover, a future experiment is to determine the effect that the distance between the lamp and the solution has on the rate of photosynthesis. Several experiments with a similar setup to this experiment that vary the distances between the lamp and solution could be used to test this.
The Effect of Wavelength on Photosynthesis Rate Aim: To be able to To investigate how different wavelengths (colors) of light affect the photosynthetic rate of the synthetic. I will use a pant that is a pond weed called elodea. I will measure the rate of photosynthesis by measuring the amount of o2 given off in bubbles per minute from the elodea. I will do this by placing the Elodea in a test tube with sodium hydrogen. carbonate then I will vary the light wavelength (color) using colored.
The Effect of Light Intensity on Photosynthesis Of Elodea Canadensis Introduction I wanted to find out how much the light intensity affected the Photosynthesis in Elodea Camadensa. I decided to do this by measuring the amount of oxygen created during photosynthesis. Photosynthesis is the procedure all plants go through to make food. This process uses Carbon dioxide, water and light energy. It produces Oxygen and Glucose.
Two members of the group were instructed to visit the laboratory each day of the experiment to water and measure the plants (Handout 1). The measurements that were preformed were to be precise and accurate by the group by organizing a standardized way to measure the plants. The plants were measured from the level of the soil, which was flat throughout all the cups, to the tip of the apical meristems. The leaves were not considered. The watering of the plants took place nearly everyday, except for the times the lab was closed. Respective of cup label, the appropriate drop of solution was added to the plant, at the very tip of the apical meristems.
The primary nutrients that plants require are carbon, hydrogen, and oxygen from air and water. Sugar has a different effect on animals, humans, and plants. In this experiment, we are testing the effects of sugar on the growth of wheatgrass. For this experiment, we used regular sugar that we put in our food and drinks. To test our hypothesis we are using two groups control and experiment group. The control plant received only water but the experimental plant received sugar but, both plants were placed in the same temperature and same amount of water. Our hypothesis was correct, experiment plant “sugar water” yield more plant growth than control plant “water”. This experiment shows the sugar water plant grow faster than water because of the average of both plants. The sugar water experiment plant had longer in length compared to water control plant.
In our Biology Lab we did a laboratory experiment on fermentation, alcohol fermentation to be exact. Alcohol fermentation is a type of fermentation that produces the alcohol ethanol and CO2. In the experiment we estimated the rate of alcohol fermentation by measuring the rate of CO2 production. Both glycolysis and fermentation consist of a series of chemical reactions, each of which is catalyzed by a specific enzyme. Two of the tables substituted some of the solution glucose for two different types of solutions. They are as followed, Table #5 substituted glucose for sucrose and Table #6 substituted the glucose for pH4. The equation for alcohol fermentation consists of 6 Carbons 12 Hydrogens 6 Oxygen to produce 2 pyruvates plus 2 ATP then finally the final reaction will be 2 CO2 plus Ethanol. In the class our controlled numbers were at Table #1; their table had 15 mL Glucose, 10 mL RO water, and 10 mL of yeast which then they placed in an incubator at 37 degrees Celsius. We each then measured our own table’s fermentation flasks every 15 mins for an hour to compare to Table #1’s controlled numbers. At
The “Fast Plant” experiment is an observation of a plants growth over the span of twenty-eight days. The objective is to observe how plants grow and use their resources throughout the span of their life. In our lab we observed the Brassica rapa, a herbaceous plant in the mustard family which has a short cycle which makes it a perfect plant to observe in this experiment. Like other plants the Brassica rapa must use the resources in the environment to create energy to complete itʻs life cycle and reproduce. By observing the plant it is easy to see in what organ or function the plant is using itʻs energy and resources and if overtime the resources switch to other part of the plants. By conducting this experiment we are able to observe where and how plants allocate their resources throughout their life by harvesting plants at different points in their life.
The Effect of Light Intensity on the Rate of Photosynthesis in an Aquatic Plant Introduction The input variable I will be investigating is light, as light is just one of the 4 factors required in the green-plant process of photosynthesis. Photosynthesis is the process by which green-plants use sunlight, carbon dioxide, water & chlorophyll to produce their own food source. This process is also affected by the temperature surrounding the plant (the species of plant we experimented with, pond weed, photosynthesised best at around 20 degrees centigrade.) Light, temperature & CO2 are known as limiting factors, and each is as important as the next in photosynthesis. Light is the factor that is linked with chlorophyll, a green pigment stored in chloroplasts found in the palisade cells, in the upper layer of leaves.
An Experiment to Investigate the Effect of Light Intensity on the Rate of Photosynthesis. Introduction Photosynthetics take place in the chloroplasts of green plant cells. It can produce simple sugars using carbon dioxide and water causing the release of sugar and oxygen. The chemical equation of photosynthesis is: [ IMAGE ] 6CO 2 + 6H20 C 6 H12 O 6 + 6O2 It has been proven many times that plants need light to be able to photosynthesize, so you can say that without light the plant would neither photosynthesize nor survive.