Carrot Lab
Abstract: In this lab, carrots were placed in sugar solutions of varying sugar concentrations. The effects of osmosis and cellular diffusion are going to be observed as the carrots gain or lose weight in the solutions over the period of time. The weights of the carrots will be measured after 30 min, and again after 24 hrs. The purpose of this lab is to find out how the cells in carrots will react to various sugar concentrations. The idea is that the cells will absorb the water and become heavier and supple.
Introduction: One of the most common types of passive transport of materials is called diffusion (Ward’s Natural Science). Another common type of transport is osmosis. Osmosis is the movement of water through a cell membrane.
…show more content…
Because of how much water the carrots should absorb, they will increase in mass because the cells will be full of the water.
Materials: 6 baby carrots, identical
6 sugar solutions
Timer
Beakers
Scale
Sugar and Distilled Water
Marker Dye
Variables: In this lab, the independent variable is the sugar concentration. The dependent variables are the weights of the carrots. The controlled variables would be the amount of water, the temperature in the room, carrot size, the time the carrots are in the solutions, etc. The experimental groups are the varying sugar solutions. The control group is the carrot is the distilled water.
Procedure:
Put 1 carrot in each solution.
Wait 30 min.
Record the weight of the carrot and see if anything changes.
Leave the carrots in the solution for 24 hrs.
Record observations. Did the carrot’s mass change at all?
Observations and Data:
Table 1. Carrot weights Before and After Soaking in Sugar Solutions.
Sugar Concentration (M)
Carrot Weight (g)
Before
After 30 min
After 24 hr
1.0 (A)
12.2
12.2
…show more content…
The hypothesis tested was rejected. It stated that all the carrots would increase in weight. Not all of the carrots increased in mass. Some of them actually lost weight, in fact (cups A, B, and C). The carrots that lost weight were in a hypertonic solution (there were more molecules outside of the cells), or the carrots in the highest sugar concentration. The water moved out of the cell because of osmosis. Only water moves through the membrane, not sugar particles because they are too big (not permeable to the membrane) (Baker, et. al., 2012). The solution that had a low concentration of sugar was a hypotonic solution. The carrot gained mass because water moved in the cells to dilute the solutes (cup D). Cups E and F were isotonic solutions. There was no net movement of water because there was equal amounts on both sides of the
The Effect of Solute Concentration on the Rate of Osmosis Aim: To test and observe how the concentration gradient between a potato and water & sugar solution will affect the rate of osmosis. Introduction: Osmosis is defined as, diffusion, or net movement, of free water molecules from high to low concentration through a semi-permeable membrane. When a substance, such as sugar (which we will be using in the experiment we are about to analyse), dissolves in water, it attracts free water molecules to itself, and in doing so, stops them from moving freely. The effect of this, is that the concentration of (free) water molecules in that environment goes down. There are less free water molecules, and therefore less water molecules to pass across a semi-permeable membrane, through which sugar molecules and other molecules attached to them are too big to diffuse across with ease.
Osmosis Experiment Planning Aim: The main subject that I will be planning to investigate is the effects of a concentrated sucrose solution on potato cells on the basis of the Osmosis theory. Background knowledge: The plant cell and its structure To understand osmosis in detail I will need to explain the plant cell (which is the cell included in the osmosis experiment) and its cell membrane. Below I have a diagram of a plant cell: [IMAGE] Osmosis is about the movement of particles from a higher concentrated solution to a lower concentrated solution to create an ethical balance via a partially or semi permeable cell membrane. Osmosis in simple terms is the exchange of particles between the cytoplasm inside the cell and the solution outside the cell. What makes this exhange is the partially permable cell membrane.
* We would have to leave one end open to fill it up with the different
Osmosis is a type of diffusion which is only applied on water and is a passive process which does not require an input of energy from the cell; this is because materials are moving with the concentration gradient. Osmosis is a process that occurs at a cellular level, which entails the spontaneous net movement of water through a selectively permeable membrane, from a region of high to low water concentration, in order to equalise the level of water in each region. This form of diffusion takes place when the molecules in a high concentration are too large to move through the membrane. The term semi-permeable or selectively permeable means that some substances can easily pass through the cell membrane, whereas others cannot. The significance of osmosis to cells is great, since it is the osmotic pressure that maintains the shape of an animal cell and provides support in the plant cells. Many factors affect the rate of osmosis including size of particles and temperature however in this particular experiment the factor investigated is the concentration of sodium chloride. Tubes of potatoes will be used to demonstrate the fact...
When a cell membrane is said to be selectively permeable, it means that the cell membrane controls what substances pass in and out through the membrane. This characteristic of cell membranes plays a great role in passive transport. Passive transport is the movement of substances across the cell membrane without any input of energy by the cell. The energy for passive transport comes entirely from kinetic energy that the molecules have. The simplest type of passive transport is diffusion, which is the movement of molecules from an area of high concentration to an area of lower concentration. Diffusion
Investigate the Osmosis of Potato Cells in Various Salt Solutions. Introduction I have been asked to investigate the effect of changing the concentration of a solution on the movement of water into and out of potato cells. I will be able to change the input of my experiment. The input variable is the concentration of the solution.
Osmosis is the facilitated diffusion of water across the cell membrane of a cell. The inside layer of the cell membrane is hydrophilic, meaning water cannot easily pass through the membrane. The cell membrane has to have aquaporins, which are water channel proteins, that move the water across the membrane. If there is a water and salt solution outside the cell, the salt can enter the cell by diffusion, but the cell membrane is not permeable to the water. Because there is more solute solution inside the cell, there is less water. The aquaporins move the water across the membrane until equilibrium is reached.
To investigate the osmotic effect of changing the concentration of sucrose solution; distilled water, 20% sucrose solution, 40% sucrose solution, 60% sucrose solution on the change in mass of potato cylinder after 30 minutes of being in solution.
Water Potential of Potato Cells Aim: To demonstrate the Water Potential of Potato Cells. Objectives: · To show the water potential of potato cells using various measured concentrations of a sucrose solution and pieces of potato. · To record and analyse data to verify observed results. · The method and procedure was carried out as per instruction sheet. Observations: The experiment shows that the lower the concentration of the sugar solution, in the Petri dish, the mass of the potato increased.
Osmosis in Carrots Background Osmosis is the diffusion of water from a dilute solution to a more concentrated solution through a partially permeable membrane, which allows the pass of water molecules but not solute molecules. [IMAGE][IMAGE][IMAGE][IMAGE][IMAGE][IMAGE][IMAGE][IMAGE]If a cell is placed in a less concentrated solution water enters because the less concentrated solution will have a high concentration of water than the inside of the cell. Once the cell takes in maximum water the cell becomes turgid. If the cell was to be placed in a high concentrated solution, water would leave the cell because the cell would contain a low concentrated solution. So in the low concentrated solution there will be a high concentration of water and in the high concentrated solution there will be a low concentration of water.
However, the solution could be more water than salt so the water from the solution could be more concentrated than the water inside the potato cells. If so then In theory the water in the solution should diffuse into the potato. cells and increase in mass. Apparatus: Potato to perform the experiment on. Thin metal tube to cut potato chops with.
Two members of the group were instructed to visit the laboratory each day of the experiment to water and measure the plants (Handout 1). The measurements that were preformed were to be precise and accurate by the group by organizing a standardized way to measure the plants. The plants were measured from the level of the soil, which was flat throughout all the cups, to the tip of the apical meristems. The leaves were not considered. The watering of the plants took place nearly everyday, except for the times the lab was closed. Respective of cup label, the appropriate drop of solution was added to the plant, at the very tip of the apical meristems.
Osmosis in Potato Tubes Osmosis: Osmosis is the movement of water molecules through a semi-permeable membrane from a high concentration to a low concentration. Diagram: [IMAGE] [IMAGE] Aim: To see the effects of different concentration of sugar solution on Osmosis in potato tubes. Key factor: In the investigation we change the sugar solution from: 0%-10%-20%-30%-40%-50% this is the independent variable; the dependant variable is the change in mass. Prediction: I predict that all the potato tubes in pure water or low concentration sugar solution will swell because water enters their cells by osmosis.
The experiment was quite reliable as I found out accurately the mass gained/lost through osmosis. However, due to the time constraints I couldn?t set up measures to ensure the temperature of each potato strip was the same and the surface area. Also I would have worn latex gloves to ensure that impurities would not go into the test tube while putting the potato strips in.
Prediction I think that when the potato is placed in distilled water the potato mass will increase. This is because water, has, if pure, a weaker concentration than the potato, and the water molecules move in through a partially permeable membrane by osmosis. If the potato is in a high concentration salt solution, the potato mass will decrease because the potato is less concentrated than the salt solution, and the water will move through the partially permeable membrane into the stronger solution. However, if the potato mass stays the same after the experiment, this means that the water/salt solution and the potato must be of equal concentration.