The research work was undertaken with the aim of study Agrolistic transformation in Sugarcane and studies of associated problems. The work was carried out in the Department of Molecular Biology and Genetic Engineering, Vasantdada Sugar Institute, Pune during Jan 2014- May 2014. The materials used and the methods adopted are presented below: Plant material used Top portion of sugarcane of age varying from 4-10 months is used as initial explants however sugarcane of more than 6 months was not preferred. The sugarcane (Co86032) explants were collected from experimental fields of MB & GE department at VSI, Pune. The parameters used for selection of the explants were apparently healthy condition and vigorous growth. Preparation of media Many seemingly unrelated factors might determine the ability of a specific tissue to regenerate into shoots and roots. Among them, mineral nutrients, plant growth regulators and environmental factors are critical. All ready standardized media for somatic embryogenesis and regeneration adopted to carry out regeneration round study. The medium consisted of macronutrients, micro nutrients, Fe-EDTA, vitamins, amino acids, sucrose/maltose, agar and plant growth regulators (auxins and cytokines). Coconut Water (CW) was used as organic additives in regeneration experiments. This was collected from tender nuts, filtered through Whatman filter paper (3 mm) and added in medium before autoclaving. Tissue culture protocol Tissue culture protocol of sugarcane consists of five stages: explant inoculation, callus induction, sub-culturing and somatic embryogenic calli production, and regeneration of shoots and rooting. Lastly, acclimatization of the plantlets in the green house. Processing of explants The young... ... middle of paper ... ...15 sec. Pipet out 10 µl aliquot of above mixture onto center of a Kapton disc. Dry at room temp. in glass petri plate Gold particle preparation Take 60mg gold Add 1 ml 100% ETOH-vortex Sonicate for 1 min Spin and remove supernatant Add 1ml SDW – Vortex Spin & remove supernatant Add 1ml SDW – Vortex Spin & remove supernatant Add 1 ml sterile Glycerol (50%)&vortex Make aliquots.50µl each Gene coating method for plasmid Take 50µl gold particles Add gene 1µg/µl conc. (plasmid) 10µl for 6shots Vortex gently Add 50µl CaCl2 and then add 20µl spermidine ( vortex) Vortex gently & keep in 4°c [vortex] for 30 min Add 200µl ETOH vortex and spin, remove supernatant Repeat step 6 for twice Add 60µl ETOH, vortex and sonicate Spread on micro-carrier for [6 preparation] GUS Assay
“Strange Fruit” by Billie Holiday conveys the inhumane, gory lynchings of African-Americans in the American South, and how this highly unnatural act had entrenched itself into the society and culture of the South, almost as if it were an agricultural crop. Although the song did not originate from Holiday, her first performance of it in 1939 in New York City and successive recording of the song became highly popular for their emotional power (“Strange fruit,” 2017). The lyrics in the song highlight the contrast between the natural beauty and apparent sophistication of the agricultural South with the brutal violence of lynchings. Holiday communicates these rather disturbing lyrics through a peculiarly serene vocal delivery, accompanied by a hymn-like
Catalase is a common enzyme that is produced in all living organisms. All living organisms are made up of cells and within the cells, enzymes function to increase the rate of chemical reactions. Enzymes function to create the same reactions using a lower amount of energy. The reactions of catalase play an important role to life, for example, it breaks down hydrogen peroxide into oxygen and water. Our group developed an experiment to test the rate of reaction of catalase in whole carrots and pinto beans with various concentrations of hydrogen peroxide. Almost all enzymes are proteins and proteins are made up of amino acids. The areas within an enzyme speed up the chemical reactions which are known as the active sites, and are also where the
The sugar maple is a very prolific seed producer. It produces a good seed crop every two to five years and can disperse 8.56 million samaras per acre in a virgin stand or 4.3 million in a selectively cut stand during an exceptional year (5). This is a large number, but only about 1 in 100 of these seeds will reach the seedling stage because of mortality, predation, competition, and the very low light levels in a sugar maple stand. Artificial regenerative measures are usually unnecessary in places such as the Lake States where the sugar maple grows naturally.
2. Put the test tube inside a beaker for support. Place the beaker on a balance pan. Set the readings on the balance to zero. Then measure 14.0g of KNO3 into the test tube.
Haiti as a rich history, filled with many peoples’ blood and countless hopes of freedom. With such a rich history, the country has yet to become financially stable because of their past. From the beginning of the French settlement where slaves were brought over from Africa to harvest crops, to the dictatorship of the Duvalier family. Haiti has yet to see a time when they are not in need of help. After the racial caste systems were set in place, many people would not see a truly independent country. When France finally gave independence to Haiti, they did it for a price. After they paid that price, the US occupied them because of their location and many resources. Every year in Haiti’s history as a nation and before has effects on the world today. These effects are not hidden in fine print, but blatantly found within Haiti and those who have been involved with Haiti.
The Republic of Cuba, as it is officially named, is a little country located in the Caribbean. Cuba is the greatest island in the Caribbean and it has over 11 million native habitants, making it be the second most populated after Hispaniola. Even though the capital of Cuba is Havana, its biggest city, the main island of it is Isla de la Juventud. Cuba also has a few of archipelagos and the second greatest city is Santiago de Cuba. The island is as close to The United States as 93 miles away and from Mexico as 130 miles away. In addition there are many countries close to the island like Jamaica, Haiti, the Dominican Republic and the Bahamas, just to name a few. In addition, Cuba is a beautiful
When the country Haiti is said, one does not think of a nice, relaxing, vacation destination. Thoughts like ‘poverty’ and ‘earthquake’ pop into mind. Haiti has been struggling its whole existance trying to overcome poverty, crime, and natural disasters. Throughout all of this, Haiti’s people and culture has remained as exciting and energetic as it naturally is. From the mountains to the coastal lowlands, Haiti’s culture and struggles make it the interesting tropical island it is today.
The apical extent of the root filling and the quality of the root filling were investigated
...ince, there is a need to use for advanced novel methods of culturing plant to furnish new means for quickly propagating,conserving of endangered species and also introducing exotic plants. The production of high quality planting material of exotic nature propagated from vegetative parts through tissue culture has created new opportunities in global trading. The exotic plants are advantageous for farmers;growers; nursery owners & rural employment. As exotic plants are restricted to their natural environment; the main benefit of tissue culture technology lies on production of high quality & uniform planting material that can be multiplied on a year round basis. The plant selected for such purpose is Stevia rabuadiana Bertoni. Objectives of study:
Tissue culture allows for the growth of a plant without the use of seeds or pollination.
Recently scientists have shown an increasing interest on the reproductive biology of tropical trees such as Leucaena (Brewbaker, 1983), the genus Acacia (Tybirk, 1989; Tybirk, 1992; Buitlaar, 1993; Diallo, 1997), Saba senegalensis (Traoré, unpubished), and the monospecific genus Faidherbia albida (Gassama-Dia et al, 2003). Regarding B. aegyptica, some preliminary studies on seed pregermination treatment and vegetative propagation were mentioned by El Nour et al. (1991). This paper presents results on the flowering, pollination and reproductive capacity in A. senegal.
Breakthrough improvements in the major grain crops have increased world food production dramatically during the last twenty seven years. The advancements in grain production, however, have not brought significant benefits to areas where root crops are the major staples. Therefore, more emphasis should be directed toward such root crops as taro, which is a staple food in many developing nations of Asia, Africa, and the Pacific.
Asexual propagation is the process through which reproduction without passage through the seed cycle occurs. The advantages of asexual propagation are that it preserves genetic makeup, propagates seedless plants, disease control, rapid production, the plants are identical, cheaper, faster and easier reducing or avoiding juvenility. The disadvantages of asexual propagation are that it increases disease and insect susceptibility, plants are bulky, and the mother plants could become contaminated. The goal of this experiment was to determine the development of adventitious roots and shoots, and observe these plants over a period of five weeks. Due to auxin being produced in the tip, tip cuttings should root faster than any other cuttings. Auxin is a plant hormone that is responsible for cell elongation and enlargement, root formation, and growth. There are two forms of auxins; phototropism, which is produced in the tip and moves downward on the side away from the light and gravitropism, which is where plant roots grow downward and plant shoots grow upward.(Plant Auxin 201...
...y lead to a chimeric offspring. The steps of developing a genetically modified crop (GMC) The steps are: “isolation of the gene of interest, indention of the gene, plant transformation, selection of the selected cells, regeneration in a large amount through tissue culture, verification of the transformation of the characterization of the inserted DNA fragment, and safety assessment” (CM Crop)
tube. Add 6 mL of 0.1M HCl to the first test tube, then 0.1M KMnO4 and