Gram Red Lab Procedure

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Recommended: Gram staining practical

All equipment’s were collected along with lab coat and safety goggles. The lab bench was cleaned with disinfectant and paper towel. The Bunsen burner was switched on and the loop was flamed and used to smear bacteria from the petri dish. Once the slide was prepared. It was then passed through a Bunsen burner three times. Crystal violet was added to the slide and incubated for one minute. The slide was rinsed with a water for at least five seconds to remove crystal violet. Gram's iodine was poured into the slide for one minute as this secures the crystal violet to the bacterial cell wall. The slide was rinsed with an alcohol for three seconds and then washed with water. The alcohol used will decolorize the sample if it is Gram negative, it

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