Computational tools provide researchers to understand physico- chemical and structural properties of proteins. A large number of computational tools are available for making predictions regarding the identification and structure prediction of proteins. Here ProtParam tool of Expasy is used to evaluate and determine various parameters. Isoelectric point (pI) is the pH at which the surface of protein is covered with charge but net charge of the protein is zero. Proteins are found to be stable and compact at their pI. The calculation of the protein charge does not include knowledge about any potential post-translational modifications the protein may have. The protein charge plot (Fig. 2) here shows that in the pH range of 6-8, the isoelectric point of all the four subunits of the enzyme lie. The computed pI value of alpha, beta and delta subunits (pI < 7) indicates that these subunits are acidic and the pI of gamma subunit (pI > 7) reveals that it is basic in character. The computed isoelectric point (pI) will be useful for developing buffer systems for purification by isoelectric focu...
In the movie A Beautiful Mind, the description of schizophrenia is shown in many accurate ways. The Diagnostic and Statistical Manual of Mental Disorders (DSM) states that the symptoms of this disease are delusions, hallucinations, disorganized speech, or unorganized or catatonic behavior. People with schizophrenia are also socially withdrawn and awkward when in contact with other people. These traits of the sickness are shown in detail throughout the movie by way of the character John Nash’s struggle with the disease. Nash is a very intelligent professor but believes he is working with the government to foil a Soviet attack plot. Nash eventually goes onto win a Nobel Prize for one of his theories. The movie shows the effects of schizophrenia on not only one man, but also on the friends and family of the ill individual. Treatment is discussed but not to any great length due to him ignoring the doctor’s orders on medication. Overall the movie shows some very prevalent traits of the disease in great detail during certain parts of the film.
The Grand Mosque of Córdoba remains to this day, one of the finest mosques, of that which once was part of the west of the Islamic Umayyad Caliphate. Córdoba was once the capital and seat of the Umayyad Caliph in Spain; then known as Al-Andalus. As such, this city was graced in the days of Islamic Rule by the building of a mosque; a most fundamental part of any Islamic city. If one were to think of Spain now, Islam is of course not the first thought that would usually come to mind. Spain is, after all, a Christian land with a deep Roman Catholic history, and the location of many Christian Pilgrimage destinations. Thus, one would be forgiven for thinking that the Islamic Empires had probably never even reached as far as West Europe. The Grand Mosque of Córdoba tells a different story.
While I was reading the novel Beloved, I noticed several testimonies throughout the book, one of them being equality. The novel tells a tragic story about slavery and it is often pointed out that the color of one’s skin determines how he or she will be treated throughout life. The slaves in the book are in constant battle to survive among the white men; however, survival is not always the best things for the slaves.
The aim of this paper is to take an in-depth look at the emotion of disgust, and explore what role (if any) it should play in morality. The issue of what role disgust should have in morality is disputed by advocates and skeptics. In what follows, I will outline this dispute and show the arguments each side makes. Following that, I will take a look into Kelly’s view which takes the skeptical stance, but differs in how he comes to take that stance. Next I will show how Kelly uses his view to disprove the disgust advocates’ arguments. Finally, I will illustrate a promising line of argument that may give hope to the disgust advocates, in light of Kelly’s powerful skeptical stance.
Our current society is not capable of turning into one similar to Gilead. Gilead is an unstable time period, for what was known to be the United States of America. There are several reasons why our society today cannot be one like Gilead. The people of Gilead do many acts that violate the Bill of Rights, which our society respects highly. The United States Constitution is also violated in the novel, The Handmaid’s Tale by Margaret Atwood.
There he stands atop the stairs of the Lincoln Memorial, in the nations’ capital, at a podium, speaking to the world. The crowds faces full of hope. Ears opened wide and clear, for the Dr. is speaking today. He is speaking from the heart and telling the nation that he has a “DREAM.” Dr. King had a dream and as most people think that it is geared towards blacks and whites, it’s not. Dr. King wanted equality between everybody. He wanted freedom from racism and prejudice throughout the nation. He was very good in getting the emotional perspective of persuasion across to his audience. King was a very inspirational man; his words were so true and full of meaning. He spoke to everyone and got everybody involved. This is why people followed him and why I think this is the most persuasive piece we have read. He not only implies his wants but he carries along with him the wants and needs of every man or woman ever treated poorly because of the color of their skin or their beliefs. There is a certain unexplainable meaning and feeling when a man like King steps up in front of the world and says, “I have a Dream.”
Have you ever wondered what was in your cigarette, cigar, cigarillo and chewing tobacco? Do you know how Tobacco affects your body? Do you know about nicotine? Tobacco contains over 200 chemicals, 60 are extremely bad for you.
You might guess it’s a custom dreamed up by a modern-day, real-life Willy Wonka, but people have been chewing gum, in various forms, since ancient times. There’s evidence that some northern Europeans were chewing birch bark tar 9,000 years ago, possibly for enjoyment as well as such medicinal purposes as relieving toothaches. The ancient Maya chewed a substance called chicle, derived from the sapodilla tree, as a way to quench thirst or fight hunger, according to “Chicle: The Chewing Gum of the Americas” by Jennifer P. Mathews. The Aztecs also used chicle and even had rules about its social acceptability. Only kids and single women were allowed to chew it in public, notes Mathews. Married women and widows could chew it privately to freshen their breath, while men could chew it in secret to clean their teeth.
Tobacco is a powerful plant that has become extraordinarily affluent and in demand worldwide. It has been one of the most commonly used drugs throughout the past few centuries and it has a very long history. It dates back to the time of the Native Americans, but became increasingly popular when the Europeans arrived to America. At the time, it became very popular for trading. People traded spices, silk, food, and more, in exchange for tobacco. There are more than 70 different strains of tobacco. There are also numerous ways for one to consume tobacco including cigars, cigarettes, chewing tobacco, and flavored tobacco. Although not everyone consumes tobacco, tobacco affects everybody. Tobacco has drastic effects on the world and these effects impact everyone. Most importantly, tobacco influences our health drastically. There is a notable comparison between a person who does not smoke tobacco and a person who does. Tobacco also impacts everything around us. It drastically impacts our economy and our surrounding environments.
n.d. - n.d. Peptides and Proteins. Proteins. Retrieved July 25, 2008, from http://www.cd http://www.cem.msu.edu/reusch/VirtualText/protein2.htm Ophardt, C. E. (2003).
In Flannery O’Connor’s short story “A Good Man is Hard to Find” the author uses foreshadowing to lead up to the unexpected twist of fate that the family finds when meeting the story’s antagonist “The Misfit.” As columnist in English Language Notes David Piwinski explains, “The murders of the grandmother and her family by the Misfit come as no surprise to the attentive reader, since O’Connor’s story is filled with incidents and details that ominously foreshadow the family’s catastrophic fate” (73). The following passage will explore O’Connor’s usage of foreshadowing in “A Good Man is Hard to Find.”
Forensic science plays a vital role in the criminal justice system by aiding an investigator’s case with scientific information based on the analysis of the evidence. Each crime scene is unique in its own way and using the evidence collected, forensic experts try to piece it together. An expert is someone who has had enough education, training, and experience to testify to the matter at hand (Harmon 2010). Unlike other witnesses in a case who testify based on first hand knowledge, the expert witness is not required to have firsthand knowledge of a particular case, and in fact, often does not. Rather, the expert witness testifies to the meaning of the facts (Whitcomb et al. 2005). Each forensic expert typically has a background in another scientific discipline, such as biology, physics, chemistry, etc. An expert with a biology background may work with DNA; chemistry may work in toxicology; and physics in blood spatter trajectories. Working separately on their own respected evidence, an investigator is able to collect all their data and set up a case (National Institute of Justice 2013). Usually, these experts will be hired by either the prosecution or defense in a criminal trial, or by a plaintiff or defendant in a civil litigation. The role of the expert witness exists in variants: between criminal and civil courts as well as the prosecution and defense.
In today’s times, kids all over the world are making different types of slimes to keep themselves entertained. Slime has been around for many years, but recently there has been new ways to make slime at home. Slime can be made using many different ingredients. Some common ingredients used to make slime include; Borax, baking soda, contact lens solution, water, and liquid starch. All of these slime recipes have one ingredient in common, glue. Kids have an array of substances they can add to their slime mixture to create more fun. Additional substances can include food coloring, glitter, Orbeez, small toys, beads, or even confetti. Does decorating slime mixtures increase the consistency of a standard slime mixture? Orbeez can make the slime feel
Proteins are considered to be the most versatile macromolecules in a living system. This is because they serve crucial functions in all biological processes. Proteins are linear polymers, and they are made up of monomer units that are called amino acids. The sequence of the amino acids linked together is referred to as the primary structure. A protein will spontaneously fold up into a 3D shape caused by the hydrogen bonding of amino acids near each other. This 3D structure is determined by the sequence of the amino acids. The 3D structure is referred to as the secondary structure. There is also a tertiary structure, which is formed by the long-range interactions of the amino acids. Protein function is directly dependent on this 3D structure.
In two-dimensional electrophoresis gel of isoelectric focusing is placed horizontally on an SDS gel , the electric field is applied and the protein macromolecules are separated acrodo with the isoelectric point and in terms of its molecular mass , providing a electrophoretogram that enables viewing and identification of many proteins at once .