Algal Pigments

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Algal Pigments All photosynthesizing plants have certain pigments in them by which they capture sunlight. All plants have in common the photosynthetic pigment of chlorophyll, but the other accessory pigments as different. These accessory pigments can be the same between two red algae, or two green algae. In this lab a red, green and brown algae will be used to extract the photosynthetic pigments from. We will also be extracting the photosynthetic pigments from Coleus sp., which is a red land plant leaf and, and compare the accessory pigments of this to the red algae. Methods and Materials We used two different kinds of red algae for this experiment. The first was Polysiphonia sp. and the second Porphyra sp. The green algae were Entreromorpha linza, and the brown algae were Fucus gardneri. The test plant we used to compare against the red algae was Coleus sp. A different procedure was conducted for extracting pigments from the different colors of algae. The red algae were grinded using both water and 90% acetone in order to get all the pigments out. The green algae was crushed in 90% acetone only, and the brown algae was placed in DMSO and then in 90% acetone. We used the same technique for Coleus sp. was as we did for the red algae. A spectrophotometric analysis was taken of each of the seven total samples. Polysiphonia sp. was not used in the spectrophotometric analysis because it did not seem to work as well as the Porphyra sp. when we were extracting the pigments. Results The attached graphs show the spectropotometric analysis of each sample taken. These graphs have the peaks labeled with their corresponding photosynthetic pigments. Below is a list of the species, and the wavelength peaks they had. Species Wavelengths Porphyra in water 418 nm, 436 nm, 496 nm, 562 nm, 620 nm, 680 nm Porphyra in acetone 414 nm, 432 nm, 480 nm, 620 nm, 664 nm Enteromorpha in acetone 434 nm, 458 nm, 664 nm, 656 nm
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