A simple and rapid reversed phase-high performance liquid chromatographic (RP-HPLC) method was proposed for simultaneous determination of imipramine hydrochloride and diazepam in pharmaceutical formulations. Elution was done utilizing methanol: water: 0.1%m sodium acetate (30:50:20 v/v/v) mobile phase with Chromosil C18 column in isocratic mode, at a flow rate of 1.0 ml/minute and detection wavelength of 243 nm. The measured retention times for imipramine hydrochloride and diazepam were 3.33±0.02 and 4.64±0.02 minutes, respectively. Validation of the method was established from linearity, robustness and recovery studies. Linearity was measured in the range 5-50 µg/ml for imipramine hydrochloride (r2=0.999) and in the range 2-20 µg/ml for diazepam (r2=0.9994), respectively. Satisfactory validation was also obtained from recovery (100.95-101.52% for imipramine hydrochloride and 99.47-100.33% for diazepam) studies, intra-day and inter-day precision (<2%) and robustness results. The reported method was the first study for quantitative determination of these drugs in combination and could be employed to investigate them in tablet dosage form in bulk.
Keywords: Imipramine HCL; Diazepam; RP-HPLC; UV detection; Tablet dosage form.
Imipramine hydrochloride (IPM), a tricyclic antidepressant belongs to anti-parkinsonian category [1]. Its chemical name is 10,l1-dihydro-5Hdibenz[b,f]azepine-5-(dimethylaminopropy1) hydrochloride. The molecular formula is C19H24N2.HCl and 316.9 gm/mol is the molar mass. It is officially recognized in Indian Pharmacopoeia (IP) [1], British Pharmacopoeia (BP) [2] and United States Pharmacopoeia (USP) [3].
Diazepam (DZM), a benzodiazepine and an antipsychotic drug possesses a strong activity against delusions an...
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...ese were optimized for sharper peaks with less asymmetry and for good resolution of IPM and DZM drugs. Initially, mobile phase volume ratio was developed. Methanol and water were tested separately and in combination as a mobile phase. Further, sodium acetate was also used. The addition of sodium acetate to methanol and water increased the pH of mobile phase. Because the mobile phase was basic due to the sodium acetate, pH was decreased by using dilute acetic acid. Elution pH was adjusted to 3.8 with diluted acetic acid. After several chromatographic runs, it was concluded that methanol: water: 0.1%m sodium acetate resulted in a better peak symmetry and good signal to noise (S/N) ratio and good separation of the chromatographic peaks. During the development phase, different pump pressures were tried and were optimized to 12.5 MPa for the stock solution. Optimization
Lexapro is obtainable as tablets or an oral solution, the most frequent use is the tablet. The identification of Lexapro tab...
Every 5 minutes, a small amount of mixture was dissolved in acetone (0.5 mL) and was spotted onto a thin layer chromatography (TLC) plate, which contained an eluent mixture of ethyl acetate (2 mL) and hexanes (8 mL). The bezaldehyde disappearance was monitored under an ultraviolet (UV) light. Water (10 mL) was added after the reaction was complete, and vacuum filtrated with a Buchner funnel. Cold ethanol (5 mL) was added drop-by-drop to the dried solid and stirred at room temperature for about 10 minutes. Then, the solution was removed from the stirrer and place in an ice bath until recrystallization. The recrystallized product was dried under vacuum filtration and the 0.057 g (0.22 mmol, 43%) product was analyzed via FTIR and 1H NMR
Stahl, S. M., & Mignon, L. (2010). Antipsychotics: Treating psychosis, mania and depression (2nd ed.).
Craig, D. Q. (2002). Pharmaceutical Applications of Micro-Thermal Analysis. Journal of Pharmaceutical Science, 91(5), 1201-1213.
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