Wait a second!
More handpicked essays just for you.
More handpicked essays just for you.
How to determine the water potential of potato tuber cells practical
How to determine the water potential of potato tuber cells practical
Don’t take our word for it - see why 10 million students trust us with their essay needs.
Determining the Water Potential of Potato Tubers using Gravimetric and Chardakov Techniques
1. INTRODUCTION:
Water potential (W) is the measure of free-energy status of water in plant cell, which is the driving force governing the movement of water into and out of the plat cell and affect various metabolic activities (O’Leary, 1970). Water potential is depends on different solute concentrations, pressure and matrix a particle; measured in Mega Pascal’s (MPa) and written as:
W = s + p + m
In plants dissolved solutes such as ions, sugars, proteins, amino acids and other substances decrease the availability of free energy and induce solute potential (S) , having a negative value. Another factor affecting W is the pressure potential (P) which could be either positive for turgor cells or zero for flaccid cells or negative for cells with active transpiration. Also the binding of water to colloids which decreases water potential is known as Matric Potential (m), it is often small and considered a component of solute potential (Saupe), so for convinces it is ignored and now the equation is:
W = s + p (1)
Water Potential can be determined by measuring the equilibrium vapor pressure, which can be measured by varied methods based on different parameters for this purpose utilizing gravimetric parameters (Length, volume and weight) or Chardakov methods of using specific gravity changes (O’Leary, 1970) or Chardakov pressure bomb chamber or Freezing Point Depression determination using hydrolytic pressure chambers or Cryoscopic or Oblioscopic methods amongst others ( Bland and Tanner, 1985). In this experiment The Gravimetric and Chardakov techniques are used for determining the W for potato tubers by incubation in inc...
... middle of paper ...
...of the water potential of stored potato-tubers. Plant Physiology 79 (3), p 891-895. Available From: http://www.plantphysiol.org/content/79/3/891
O’Leary J. W. (1970). A Critical Evaluation of Tissue-Immersion Method for Measurement of Plant Water Potential. Ohio J. of Sci., 70(1), p 34-38. Available from: http://kb.osu.edu/dspace/handle/1811/5510 33 [Access date: April 12, 2014]
Ros Barcelo A., Calderon A. and Munoz R. (1994). Measuring water conductivity coefficients in plant tissues. Journal of Biological Education, 28 (2), p 83 – 85. from: http://www.tandfonline.com/doi/abs/10.1080/00219266.1994.9655371 [Access date: April 12, 2014]
Saupe S.G. Measuring the Water status of Potato tubers. St. John’s University, Minnesota. [Online] Available from: http://employees.csbsju.edu/ssaupe/biol327/Lab/water/water-lab-intro.htm [Access date: April 12, 2014]
Conclusion In my conclusion, the potatoes with the lowest concentration gained the most mass, and would become hard relating back to the Turgor theory I stated earlier. In contrast to this, the potatoes in the most concentrated solution lost the most weight thus becoming plasmolysed and limp also relating back to the background I have mentioned earlier. Evaluation In general the experiment was succesful the results were consistent and also were in accordance with the theories made at the start.. The experiment could have been improved by: · More subjects used instead of potatoes · More potatoes · Wider time ranger · Different molarities Using this variety of methods could have improved the experiemnt, however I was generally satisfied with the results of this osmosis experiement.
Snyder, R. (2009). Water In The Greenhouse. Growing Produce. Retrieved on March 20, 2014 from http://www.growingproduce.com/uncategorized/water-in-the-greenhouse/
Investigate the Osmosis of Potato Cells in Various Salt Solutions. Introduction I have been asked to investigate the effect of changing the concentration of a solution on the movement of water into and out of potato cells. I will be able to change the input of my experiment. The input variable is the concentration of the solution.
Water Potential of Potato Cells Aim: To demonstrate the Water Potential of Potato Cells. Objectives: · To show the water potential of potato cells using various measured concentrations of a sucrose solution and pieces of potato. · To record and analyse data to verify observed results. · The method and procedure was carried out as per instruction sheet. Observations: The experiment shows that the lower the concentration of the sugar solution, in the Petri dish, the mass of the potato increased.
The effect of water quality on Vegetative measurements and photosynthesis indices of the trees are presented in Table 1. No significant differences were found between parameters: gs, shoot number in both years, but others were higher in RW application. Height of trees and leaf area in first year was not significant but in second year it increased by 42 and 29% respectively in application RW. Photosynthesis rate appears significantly higher (23.4 µmol m−2 s−1) in plants receiving recycle water than clean water.
However, the solution could be more water than salt so the water from the solution could be more concentrated than the water inside the potato cells. If so then In theory the water in the solution should diffuse into the potato. cells and increase in mass. Apparatus: Potato to perform the experiment on. Thin metal tube to cut potato chops with.
... els of sucrose solution to distilled water (see molarity table above) and I placed each of the mixtures into the correct beakers. 5. Then I weighed all the potato chips on an electronic balance (see results) and recorded the results. 6. I placed 5 pieces of potato into each beaker and left them for approx 36 hrs. 7. After this time I drained out the solutions from the beakers and I carefully placed them in order of molarity on a paper towel. 8. I dried the potato chips gently and then weighed each potato piece and recorded the results. 9. As I had extra time I made a second experiment and also recorded those Results: Molarity (M) Starting Weight (g) (Results 1) Final Weight (g) (Results 1) Percentage Change (%) (Results 1) Start Weight (g) (Results 2) Final Weight (g) (Results 2) Percentage Change (%) (Results 2) These values clearly support my prediction, and even though there are some anomalous results, there is an overall negative trend across the whole set of results, proving them accurate. Analysis of Results: The sucrose concentration of the solution into which the potato tissue is placed affects to what degree it grows or shrinks. As you can see from the graph the results show a clear negative correlation, a very obvious inversely proportional trend. From this a conclusion can be drawn. When the water concentration is high, the potato gains water, as seen by the 0.0 molarity solution. When the water concentration is low, the potato loses weight and therefore decreases in mass, as seen in the 1.0 concentration solution. This proves my hypothesis correct. The results were quite widely ranged, as seen on the graph, wit ......
Determining the Concentration of the Cell Sap in Potato Storage Tissue Aim: To determine the concentration of the cell sap in potato storage tissue. By using Osmosis, determine what the sugar concentration of cell sap is. Prediction I predict that the potato segment in the distilled water will definitely gain in weight because the solution outside it has a much higher concentration of water then in the cell sap meaning Osmosis will occur and the potato segment take in water. I predict that 0.2M sugar solution will also gain weight because it still has quite a high concentration of water outside the potato. The potato in the 0.4M solution will gain weight but the potato in the 0.6M and 0.8M solutions will lose weight.
We then cut our potato tubes with the cork borer and cut them with the scalpel so they were the same length and weighed them. We then put one potato tube in each test tube and then added the same amount sugar solution in to each tube. The concentration of sugar solutions varied in each test tube.
potato cells. In order to find the best way to do this experiment I am
An Experiment to Investigate Osmosis in Plant Tissue. Aim: To conduct an investigation to compare the osmotic behavior of the osmotic animal. two types of plant tissue in varying concentrations of sucrose. solution. Then we can find the solution.
The Effect of Water Concentration on the Mass of Potato Tissue Aim: The aim of this investigation is to discover the effect of water concentration on the mass of potato tissue, and also to investigate the movement of osmosis through potato tissue. Scientific Knowledge: When a substance such as a sugar dissolves in water, the sugar molecules attract some of the water molecules and stop them moving freely. This, in effect, reduces the concentration of water molecules.
the mass of the potato cylinder as well as the length as the mass can
Equipment Potato, Borer, Beakers, Measuring Cylinder, Stopclocks, Distilled Water, Electronic Balance, Salt solutions of various concentrations. Diagram [IMAGE] [IMAGE] [IMAGE] When we leave the potato in the solution for the allocated time, water
Potato is one of the most nutritious and commonly edible foods in our society today. But there is a fascinating story of how Potato came to be widely consumed today around the world today. Potato also known as Solanum Tuberosum for its scientific identification was a wild plant. It was first domesticated in 10,000 BCE around the Lake Titicaca basin in the Andes region by the farmers. This region was not the best for agriculture because of the terrain but Potato rose to the occasion to become the most dominant food. Potteries of the Andean civilization depicted Potato as the chief crop. This region is in present day Southern Peru and Northwest Bolivia. The warm summer days enhanced the growth of plants and cold nights improved the growth of tubers; hence Potato was able to survive in this region. The farmers with their trial and error method figured that Potato can be bred through its seed. The seed of a Potato produce different fruits with varying sizes and color. The most significant of the domesticated Potato by the Andes farmers was the Solanum Tuberosum Andigena. This Potato had large tubers, well rounded and can grow only near the equator. Pre-Columbian population grew over 200 different types of Potato, making this plant few of the widely domesticated foods worldwide. The planting of Potato occurred at different elevations which enabled Andean farmers to plant and harvest it all through the year. Indigenous people of South America preserved Potato and stored it in case of a famine. The cold dry climate of the Andes region made this long-time preservation possible. The process involved covering of already harvested Potatoes from mist and leaving them in the cold night. The next day with sunshine on the Pota...