The Nitrate Reduction and Denitrification test detects for the reduction of nitrate to nitrite by testing for the presence of nitrite. Anaerobic respiration involves the reduction of an inorganic molecule, like nitrate reduction, from the lack of oxygen. Many nitrifiers, bacterial species that contain the enzyme nitrate reductase that allows them to reduce nitrate to nitrite in one step (Slonczewski & Foster, 2015). Other bacteria are capable of further reducing nitrite into ammonia, nitrous oxide, or nitrogen thro...
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...ery strong and stable acids. The strong acids that are produced causes the pH to become very low. The mixed acid fermentation pathway produces acids such as acetic acid, lactic, succinic, and formic acid (Department of Biological Sciences, 2015). The medium used in this test is the same as the VP Test, which is MR-VP broth. Methyl red dye, a pH indicator, is added to the broth and only detects very strong acids. The color is red at a pH of 4.4 and yellow at a pH of 6.0. Organisms who do not use the mixed acid fermentation pathway, end up producing acids that are unstable, which end up being converted to more neutral end products and therefore changing the pH indicator to yellow. In order for the red color to be maintained, there must be large quantities of the strong and stable acid produced from the fermentation of glucose (Department of Biological Sciences, 2015).
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