Essay Isolation and Identification of Thermophilic Fungi

Essay Isolation and Identification of Thermophilic Fungi

Length: 1302 words (3.7 double-spaced pages)

Rating: Strong Essays

Open Document

Essay Preview

We isolated thirty thermophilic fungi with ability to grow at 45 °C on medium containing wheat bran and casein as carbon sources and produced proteases in both SSF and SmF (data not shown). The fungus strain F.2.1.4 presented the highest protease activity and was sub-jected to taxonomic characterization.
Analyses of macro and micro-morphological characteristics accommodated the isolate F.2.1.4 in the genus Myceliophthora due to the presence of spreading colonies with dense aerial mycelium and blastic conidia often borne in ampulliform swellings (Van Oorschot, 1980). Particularly, this isolate produced pulverulent pale mycelium, obovoid conida measuring 5.65-7.91 x 3.39-4.52 μm as well as conidia walls finely ornamented. Based on such char-acters the isolate F.2.1.4 is similar to Myceliophthora thermophila, however, some slight morphological differences were observed, for instance, M. thermophila has pale brown, woolly colonies and a variable range of conidia sizes 4.5-11 x 3.0-4.5 µm (Van Oorschot, 1977).
Data derived from BLAST (ITS-rDNA region) showed that the isolate F.2.1.4 had high sequence similarities (99-96%) with three unidentified Myceliophthora spp. isolated from geothermal soils in China; and 95-94% of sequence similarities with different strains of the Ascomycota genus Corynascus (one of the Myceliophthora teleomorphic states), includ-ing three new species: C. verrucosus sp. nov., C. similis sp. nov., C. sepedonium, C. sexualis sp. nov. (Stchigel et al., 2000). The phylogenetic tree showed that the isolate F.2.1.4 clustered with the three Myceliophthora strains from China (Figure 1).
Molecular and phylogenetic results corroborate the data from morphological analyses and considering the combined methods used for f...

... middle of paper ...

...ease immobilized in the alginate beads
When comparing the performance of immobilized biocatalysts intended for industrial use, knowledge of their operational stability is very important. The stability of an immobilized enzyme without appreciable loss of enzyme activity is important mainly for the economic viability. Figure 5 shows the effect of repeated use on the activity of the immobilized prote-ase; it decreased gradually during 7 cycles of reuse (being its activity at the 7th cycle above 50% of the original activity) and more sharply after that.
In conclusion, the thermophilic fungus Myceliophthora sp. was able to produce alkaline and thermostable proteases in SSF using wheat bran as culture medium. The protease immobilization in alginate not only maintained some properties, but improved strongly its thermal stability and permitted its reuse for seven cycles.

Need Writing Help?

Get feedback on grammar, clarity, concision and logic instantly.

Check your paper »

Thermophilic Fungi Essay

- Thermophilic fungi are known to produce thermostable enzymes which have a number of applications, mainly in biotechnological processes. In this work, we report the characterization of a protease produced in state-solid (SSF) and submerged (SmF) fermentations by a newly isolated thermophilic fungus identified as a putative new species in the genus My-celiophthora. Enzyme-production rate was evaluated for both fermentation processes, and in SSF, using a media composed by a mixture of wheat bran and casein, the proteolytic output was 4.5-fold larger than that obtained in SmF....   [tags: biology, science, experiments,]

Strong Essays
1881 words (5.4 pages)

Essay about Fungi: A Misunderstood Marvel

- Very few view fungi as essential to human life. If asked to consider this diverse kingdom, most would only answer with whether they enjoy mushrooms on their pizzanotwithstanding the fact that there are at the very least 1 million species of fungi thought to outnumber vascular plants by a ratio somewhere between six to one and thirty-three to one. Even so, only 100,000 species of fungi are known to exist while mycologists continue to discover 1000 new species every year. Most notable was the recent discovery of a single honey mushroom congregation thought to weigh 605 tons and cover 2200 acres of Oregon’s Strawberry Mountains....   [tags: Fungi]

Strong Essays
906 words (2.6 pages)

Thermophilic Conifer in Tabbowa Beds Sri Lanka Essay

- Plant fossils are found in the well known Jurassic sedimentary basin in the Puttalum district, Northwestern Province, Sri Lanka. Fossil specimens were collected at a Tabbowa basin and the morphological characters of leaves, shoots and stems, were studied. Specimens could be diagnosed as conifers belonging to two genera Brachyphyllum and Pagiophyllum. The two genera closely resemble one another in foliar morphology. However, combined transmitted light and polarizing microscopic observations together revealed additional characters such as the pattern of leaf arrangement, cushions and keel size, shape of leaf apices and bases....   [tags: Brachyphyllum, Thermorphilic, Palaeo-environment]

Strong Essays
1245 words (3.6 pages)

Phylogenetic Analysis of Thermophilic Bacteria Essay examples

- We report the community of thermophilic bacteria cultivated from Tanjung Sakti Hot Spring in South Sumatera Indonesia that has temperature 80 – 91 0C and pH 7 – 8. Based on phylogenetic analysis, the 16 sequences of 16S rRNA gene fragments obtained from the community clustered within four distinct genera as Anoxybacillus, Geobacillus, Brevibacillus, and Bacillus. Two sequences that have 96% similarity with data sequences in GenBank, are potentially as novel species/sub species. Hot spring is a unique area that characterized by high temperature and has a great diversity of natural environments....   [tags: Bacteria, Tanjung Sakti Hot Spring, South Sumatera]

Strong Essays
1559 words (4.5 pages)

Virus, Bacteria, And Fungi Essay

- What are some common pathogens that cause head, eyes, ears, nose, and throat (HEENT) infections. The common pathogens that are responsible for HEENT includes virus, bacteria,and fungi (Waitzman, 2014 para 1) Viral agents (Burns, Dunn, Brady, Starr, & Blosser, 2013, para 2 ). Influenza A, B parainfluenza adenovirus coronavirus rhinovirus human papillomavirus cytomegalovirus varicella-zoster virus herpes simplex virus respiratory syncytial virus coxsackievirus Bacterial agents: (Waitzman, 2014 para 1) β-hemolytic streptococcus, Streptococcus pneumoniae, Haemophilus influenzae, tuberculosis (TB), leprosy, Moraxella catarrhalis, Mycoplasma pneumoniae, Chlamydophila pneumoniae...   [tags: Antibiotic resistance, Bacteria, Antibiotic]

Strong Essays
726 words (2.1 pages)

The Benefits of Wearable Identification Tags Essay

- Purpose of Report The purpose of this report is to outline the benefits that an investment in wearable identification tags could bring to the business. This organisation is very human resource intensive and this technology will save resources in this department which will directly benefit the business. Assumptions In preparing this business case, I have made several assumptions regarding the implementation of this technology: • As this is an international company, each separate office has a HR presence and processes time-sheeting locally....   [tags: Radio Frequency Identification Tags]

Strong Essays
1900 words (5.4 pages)

Radio Frequency Identification (RFID) Essay

- The innovation of the RFID chip or the Radio Frequency Identification has been a critical piece of technology that has been around since the early/mid 20th century (est 1938). Functions of a radio frequency identification (RFID) tagging system involves a tag and a reader. When scanned, the antenna within the tag picks up the radio wave and sends a response back to the reader. This technology is being used for both short range and also long range identification, in the short-range identification category, this innovation is used in practical applications like credit cards (pay wave, tap and go ect...), animal identification (domestic pets, farm animals)....   [tags: Radio Frequency Identification]

Strong Essays
1939 words (5.5 pages)

The Radio Frequency Identification Technology Essay

- This project gives an overview of the radio frequency identification technology and the limitations of the usage of RFID technology. Radio Frequency Identification is the wireless technology that uses radio waves to identify objects within its proximity range. This process involves tagging an object to a transmitter which will emit a burst of information including identification of the tag. The identification process and the various components involved in the transmission, identification and receiving of the radio waves are discussed....   [tags: RFID, Radio-frequency identification, Wireless]

Strong Essays
1159 words (3.3 pages)

Bioluminescent Fungi Essay

- Bioluminescent Fungi INTRODUCTION What is Bioluminescence. The current paper main focus is on bioluminescent Fungi but the basic features of bioluminescence discussed are common to all bioluminescent organisms. Bioluminescence is simply light created by living organisms. Probably the most commonly known example of bioluminescence by North Americans is the firefly, which lights its abdomen during its mating season to communicate with potential mates. This bioluminescent ability occurs in 25 different phyla many of which are totally unrelated and diverse with the phylum Fungi included in this list (an illustration of a bioluminescent fungi is displayed in figure 1)....   [tags: essays papers]

Free Essays
2033 words (5.8 pages)

Radio Frequency Identification (RFID) Essay

- Radio Frequency Identification (RFID) Radio frequency identification, or RFID, is a generic term for technologies that use radio waves to automatically identify people or objects. There are several methods of identification, but the most common is to store a serial number that identifies a person or object, and perhaps other information, on a microchip that is attached to an antenna (the chip and the antenna together are called an RFID transponder or an RFID tag). The antenna enables the chip to transmit the identification information to a reader....   [tags: microchip radio waves identification]

Strong Essays
785 words (2.2 pages)