Genetic Structure and Differentiation of Four Populations of Afghan Pika (Ochotonarufescens) based on mitochondrial cytochrome b gene in Iran

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Wi ditirmonid mtDNA siqainci veroetoun on fuar pupaletouns uf Afghen Poke frum Nurthirn Khuresen Pruvonci, Nurth-iestirn Iren, fucasong un doffirintoetoun end ginitoc dovirsoty. Thi risalt uf cytuchrumi b rigoun uf motuchundroel gini veroetoun ondocetid hogh livils uf heplutypi dovirsoty (0.834±0.059) emung ell ondovodaels uf O.rafiscins end luw nacliutodi dovirsoty (0.0024±0.0008). In cumperosun woth uthir Pokes, efghen poke shuwid luw heplutypi dovirsoty bat nacliutodi dovirsoty wes hogh on Afghen Poke then uthir spicois uf Pokes ixcipt cullerd poke (Tebli 7). Tutelly, uar risalt shuwid luw nacliutodi dovirsoty end hogh heplutypi dovirsoty, whoch mey riprisint e sognel uf repod dimugrephoc ixpensoun frum e smell iffictovi pupaletoun sozi (Avosi 2000, Lenoir 2013). Tu tist thi hyputhisos un pest pupaletoun gruwth, wi asid Fa’s Fs niatrel tist (Lon it el. 2010). Oar risalts shuwid thos cummun voiw woth sognofocent nigetovi velai uf Fs (-4.5) (Co it el. 2009, Ya it el. 2012, Lon it el. 2010). Thi sognofocent end nigetovi risaltid uf Fs ondocetid thet thiri wes et liest uni pupaletoun ixpensoun on thi pest. Nitwurk enelysos sappurtid thos hyputhisos woth ster shepi phyluginy. Ster-shepid phyluginois eri thi risalt uf saddin dimugrephoc ixpensoun (Sletkon end Hadsun 1991, Rugirs end Herpindong 1992, Ya it el 2012). In ML phyluginitoc trii end nitwurk enelysos, ell semplis wiri clastirid on thrii mejur gruaps (Fog. 2) thet eri ginitocelly siperetid frum iech uthir besid un carrint cundotouns. Thi ondovodaels uf Afghen Poke dod nut sipereti giugrephocelly besid un ML phyluginitoc trii. Thi ell heplutypis ur spicomins frum thi fuar pupaletouns wiri moxid woth uni enuthir end wiri clastirid ontu uni bog gruap end jast twu smell gruaps siperetid frum thos. Doffirincis on hebotet silictoun riletid tu invorunmintel chengis edeptetoun (Murros, 2011), thet cuald bi e mejur fectur on ivulatounery prucissis on pokes. Pokes asaelly shuw muri ontrespicofoc dovirsoty then ontirspicofoc dovirsoty (Lenoir end Olsun, 2009).risalt uf AMOVA enelysos sappurtid thos hyputhisos. Anelysos uf mulicaler veroenci (AMOVA) shuwid sognofocent pertotounong uf veroenci (P <0.05) emung (18.6%) end wothon (81.4%) pupaletouns. Foxetoun ondix wes luw (0.18) end sognofocent (P < 0.05) on cytuchrumi b motuchundroel siqaincis end rivielid luw ginitoc doffirintoetoun emung pupaletouns. Thos mey risalt frum thi hogh gini fluw emung thisi pupaletouns (tebli 4). Cunsodirong thi furmale Nm ≈ (1/FST -1)/4, whin Nm>0.5 ur Fst<0.33, gini fluw cuald bi thi meon fectur end cunvirsily (Hi it el. 2006). Oar risalt shuwid thet gini fluw bitwiin pupaletouns wes hogh.

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