Also, the hook was used for attachment of the hanger with the slotted weight. Theory: In static equilibrium, force of a spring is proportional to and directed opposite to the elongation. This is represented by Hooke?s Law where the restoring force is equal to elongation distance from equilibrium multiplied by the constant force of the body. From that equation, the experimenter will know how much force is needed to be applied to the spring in order to stretch it a particular distance. The experiment also deals with dynamic oscillation that deals with the period of oscillation, which is independent of displacement.
• The beaker was covered with the foil and stirred constantly on magnetic stirrer overnight (ideally 8 hrs) • After overnight extraction magnetic bead was removed and the extraction buffer was centrifuge... ... middle of paper ... ...ne (TMB) reagent • Stop solution 1. 2 N sulphuric acid • NUNC flat base ELISA wells PROCEDURE: • Prepare capture antibody solution of dilution 1:250 in coating buffer and add 100 µl in each well and incubate at 40C overnight. • Next morning aspirate and wash the wells 3 times with PBST • After washing add 200 µl blocking solution which is 10 % FBS in PBS in each well and incubate for 1 hour. • Aspirate and wash 3 times. • Add 100 µl standard and samples to the allotted wells.
Hooke's Law I have designed the experiment to measure spring constants when the springs are in series and in parallel. The theory is based on Hooke's law which is: F = kx where F = Force, k = Constant and x = Extension [Ref. 1]. Unfortunately with the springs I have, I can only measure extension, not compression for which Hooke's is also valid. Prediction Single Spring: Hooke's law, where F = kx.
• Hang 100 grams of weight at the 45 cm mark, and 500 grams at the 90 cm mark on the meter stick. • Hang 200 grams of weight between 0 – 45 cm mark and move this weight until equilibrium is achieved. Record this measurement. Data Part A: Mass of weight (m-2) = 100 grams Position string balanced = 36.4 cm Distance from center of meter stick to balance point. (L-1) = 13.6 cm Distance from balance point to suspended weight.
Investigating Energy Stored in a Spring What is the spring constant? ============================ Planning Aim - to gain an average compression rate of the spring in the trolley in order to find the spring constant. Apparatus Clamp stand 2 clamps 2 bosses, 24 0.98N weights 2 weight holders 3 labels Pencil Sprit level Trolley Ruler (measures to nearest 5 x 10-4m) Diagram Plan - I am going to investigate the spring constant of the spring in the trolley to enable me to calculate the energy stored in the spring in the major investigation. To calculate the spring constant, I need to plot my results onto a graph and draw a line of best fit. The spring constant is equal to the gradient of this straight line.
After centrifugation at 12000 g for 15 min The mass of each fruit was weighed before cold storage using a weighing scale. After cold storage the fruits were weighed every 7 days. The fruit mass loss was calculated as a percentage with respect to the initial mass (mass before cold storage). Fruit firmness The avocado fruit firmness was measured using a firmness tester, firmness was measured every seven days during the cold storage period and during the shelf life. The firm tester has a scale that ranges from 100 to 10.
Examples of repetition training are: 4 x 200metres with 4 minutes rest, and 6 x 100metres with 2 minutes rest. Repetition training sets are usually done once per week within an eight-week training program. Training intensity varies from 90 to 100% of an athlete’s maximum intensity with a heart rate level above 180bpm.
In order to standardize this solution we had to weigh out 5g of KHP, which is the amount needed to neutralize 35ml of our base. Once we weighed out the KHP we then dissolved it in 50 ml of water, and finally titrated the base three different times, we could then take the average and find the molarity of the NaOH solution. After the bottles hadbeen sitting for 2 weeks we titrated each one with the base and recorded how much base was needed in (ml). Once we titrated all the bottles we then had all theinformation we needed in order to finish the calculations. Intro In this lab we are trying to determine and equilibrium constant of an ester hydrolysis reaction.
Spring constant; The spring constant will be useful to confirm the relationship. A simple force - extension experiment should be performed to get an accurate value for k which can be compared to the value of k from the final experiment. Amplitude; The amplitude of the oscillations should be kept constant. Bear in mind the amplitude cannot be larger than the extension caused by the smallest mass applied to the spring as this would not allow the system to oscillate properly.
Equal volume of chloroform was added and centrifuged at 13000 rpm for 5 minutes. Again, the aqueous solution was transferred to a new 1.5 ml microcentrifuged tube and 1/10 volume of 3M Sodium Acetate Solution was added. Then, 2.5 volumes of cold absoluted ethanol were added to precipitate the DNA. The mixture was incubated in -20 °C for overnight or -80 °C for 1-2 hour. After incubation, the mixture was then spinning at 13000 rpm for 30 minutes at 4 °C.