Investigating the Rate of Transpiration in a Mesophyte Plant Experiment to investigate the relationship between the number of
stomatal pores on the upper and the lower surfacesof the leaves of a
mesophyte plant and the rate of transpiration from those surfaces.
The aim of the experiment
The aim of the experiment is to investigate how the number of stomatal
pores is related to the rate at which water is lost from the leaves. A
mesophyte plant is chosen and the comparison is between the upper and
the lower surfaces of its leaves.
Experimental hypothesis
Taking into account the relative background scientific Information, it
is expected to be proven that the rate of transpiration from a leaf of
a plant is proportional to the number of stomatal pores on the surface
of that leaf.
Null hypothesis
Negative results would be to establish that the transpiration rate is
inversely
proportional to the number of stomatal pores or is not affected by it
whatsoever.
Introduction
Water is the universal solvent for a huge amount of chemical
substances in all living organisms. Plants require water for many
different reasons. It is used to uptake inorganic minerals from the
ground, to transport nutrients such as amino acids and carbohydrates
along their stems and to control their temperature. Water plays a very
significant role in the life cycle of plants being a vital assumption
for their life.
Plants take up water by the younger parts of the roots. Water then
moves across the cortex of the root towards the c...
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...ttach the blue cobalt
chloride paper on the leaf by a sellotape piece and start timing.
Observe the colour change of the cobalt chloride paper as water
evaporating from the leaf turns it pink from blue and for more
accuracy, compare with a moist sample. Stop timing as soon as the blue
colour is lost. Repeat the procedure for five times on each surface of
this leaf, then proceed to another leaf until all ten are examined.
Record the results into a table.
Using a calculator obtain random coordinates on upper and lower
surfaces of the leaves and apply a thin layer of nail polish on those
sample areas. Leave to dry for 10 to 15 minutes, then remove the layer
by means of forceps and transfer onto microscope slides. Count the
number of stomata on a fixed area under light microscope. Record the
results into a table.
Cellular respiration is the process by which energy is harvested involving the oxidation of organic compounds to extract energy from chemical bonds (Raven & Johnson, 2014). There are two types of cellular respiration which include anaerobic respiration, which can be done without oxygen, and aerobic respiration, which requires oxygen. The purpose of this experiment is to determine whether Phaseolus lunatus, also known as dormant seeds or lima beans, respire. You will compare the results of the respiration rate of the dormant seeds, and the Pisum sativum, or garden peas. In this experiment, you will use two constants which will be the temperature of the water and the time each set of peas are soaked and recorded. Using these constants will help
Two members of the group were instructed to visit the laboratory each day of the experiment to water and measure the plants (Handout 1). The measurements that were preformed were to be precise and accurate by the group by organizing a standardized way to measure the plants. The plants were measured from the level of the soil, which was flat throughout all the cups, to the tip of the apical meristems. The leaves were not considered. The watering of the plants took place nearly everyday, except for the times the lab was closed. Respective of cup label, the appropriate drop of solution was added to the plant, at the very tip of the apical meristems.
We placed elodea plants into three different beakers and labelled them. Since, we are trying to find how temperature can affect the rate of production of carbon dioxide, we had to place them in different temperatures. So, we labelled the first beaker “Elodea heat” and placed it in a water bath that produced sufficient amount of heat. We labelled the second one “Elodea cool” which was placed in an ice bath filled with ice. The next one “Elodea RT” where the elodea was placed under normal room temperature without any interference. And we named the last one “No Elodea” where we placed no elodea in it and kept the beaker in a dark
help give a better idea of how the rate of osmosis is affected by the
Investigating the Effect of Light Intensity on Photosynthesis in a Pondweed Aim: To investigate how the rate of photosynthesis changes at different light intensities, with a pondweed. Prediction: I predict that the oxygen bubbles will decrease when the lamp is further away from the measuring cylinder, because light intensity is a factor of photosynthesis. The plant may stop photosynthesising when the pondweed is at the furthest distance from the lamp (8cm). Without light, the plant will stop the photosynthesising process, because, light is a limited factor. However once a particular light intensity is reached the rate of photosynthesis stays constant, even if the light intensity is the greatest.
The “Fast Plant” experiment is an observation of a plants growth over the span of twenty-eight days. The objective is to observe how plants grow and use their resources throughout the span of their life. In our lab we observed the Brassica rapa, a herbaceous plant in the mustard family which has a short cycle which makes it a perfect plant to observe in this experiment. Like other plants the Brassica rapa must use the resources in the environment to create energy to complete itʻs life cycle and reproduce. By observing the plant it is easy to see in what organ or function the plant is using itʻs energy and resources and if overtime the resources switch to other part of the plants. By conducting this experiment we are able to observe where and how plants allocate their resources throughout their life by harvesting plants at different points in their life.
These leaves will also have no threat of excessive transpiration because the temperature in the shaded area will be lower and the humidity probably higher. Transpiration is the removal (evaporation) of water from a plant through the stomata in the leaves; this water is removed in a cycle due to the active uptake from the roots. Transpiration involves osmosis; which is the diffusion of water from a high concentration to a lower concentration through a partially permeable membrane, until both the concentrations are equally saturated. All these factors i.e. transpiration and photosynthesis, come together to confirm my hypothesis. To support my hypothesis further, I did a pilot study in a meadow in which I studied the population of certain plant species in areas of
Experiment #1: The purpose of this experiment is to investigate the effects of baking soda and light intensity on the rate of photosynthesis of green spinach leave through the observation of floating disk.
The Effect of Light Intensity on the Rate of Oxygen Production in a Plant While Photosynthesis is Taking Place
During the following lab, an aquatic plant was covered with a funnel and was placed underwater inside a beaker, with a graduated cylinder (submerged in water) was placed over the neck of the funnel. This lab tested out which source of light which is the independent variable would allow the aquatic plant to produce the most bubbles (dependent variable) and photosynthesize the fastest. This is clearly displayed because when photosynthesis is produced underwater it appears in the form of bubbles inside the water. These bubbles will travel up through the funnel and displace the water in the graduated cylinder. The dependent variable was tested by placing the four, 5cm aquatic plants in the beakers directly in front of the sources of light and observing how many bubbles were produced within the period of 10 minutes and how much water was displaced by the oxygen bubbles. The beaker in front of the light source with the most bubbles produced and with the most displaced water performed photosynthesis faster than all the other light sources. The sources of light used for this lab were sunlight, florescent, red, and yellow lamp lights, and
Osmosis is a type of diffusion which is only applied on water and is a passive process which does not require an input of energy from the cell; this is because materials are moving with the concentration gradient. Osmosis is a process that occurs at a cellular level, which entails the spontaneous net movement of water through a selectively permeable membrane, from a region of high to low water concentration, in order to equalise the level of water in each region. This form of diffusion takes place when the molecules in a high concentration are too large to move through the membrane. The term semi-permeable or selectively permeable means that some substances can easily pass through the cell membrane, whereas others cannot. The significance of osmosis to cells is great, since it is the osmotic pressure that maintains the shape of an animal cell and provides support in the plant cells. Many factors affect the rate of osmosis including size of particles and temperature however in this particular experiment the factor investigated is the concentration of sodium chloride. Tubes of potatoes will be used to demonstrate the fact...
To make sure that my test is fair I will make sure to keep all the
The cause of this change in mass is called osmosis. Osmosis is the movement of water. through cells in plants. I make my prediction on the pretext that water diffuses from high concentration cells to low concentration. cells.
An Experiment to Investigate the Effect of Light Intensity on the Rate of Photosynthesis. Introduction Photosynthetics take place in the chloroplasts of green plant cells. It can produce simple sugars using carbon dioxide and water causing the release of sugar and oxygen. The chemical equation of photosynthesis is: [ IMAGE ] 6CO 2 + 6H20 C 6 H12 O 6 + 6O2 It has been proven many times that plants need light to be able to photosynthesize, so you can say that without light the plant would neither photosynthesize nor survive.
Plants also had to adapt on the surface in order to survive the climate change of moving onto land. The changes made to the surface of plants are most closely observed by their formation of a cuticular wax. This waxy cuticle is impermeable to water and acts as a method of controlling plant’s water intake. It can be made thinner or thicker depending on the plant’s needs and the environment at the time, changing in response to droughts or excessive amounts of rain.