• Section A: Scoring flies o This was difficult due to the fact they we couldn’t keep the flies knocked out for long, so it took multiple trips to the fridge and even gassed them with CO2 to knock them out long enough for us to count them. o We divided them into groups be sex (male/female), and then by the desired phenotype; eye color (red/white) and body color (yellow/Ebony). • Section B: Mating Fruit Flies o We made trial vials, each containing two males and two females of a specified phenotype. In order to preserve the purity of the lineage, we did each trial twice; one with virgin females, and another with non-virgin females, creating a grand total of eight trial vials. o The vials were set up, two sets of each, as follows: Two Males with red eyes, two Females with …show more content…
(10) We learned how to extract and partially “clean” DNA from fruit flies, Inversion polymorphism associated with the ebony phenotype. • Section A: Proteinase K o The use of Proteinase K and Homogenization Buffer (HB) is used to cause the cell to lyse, breaking down the lipid cell membrane, digesting unwanted proteins and removing contamination that might otherwise degrade the DNA. This allows for the DNA to become free floating. • Section B: Precipitation o A salt treatment of NaCl clumps together non-DNA debris. It also allows for the neutralization of the negative charge in the DNA, allowing the multiple DNA strands to converge. It is then separated using the Centrifuge to spin down debris. o The DNA is insoluble in the 100% Ethanol and will come out of solution. The debris will settle at the bottom as a pellet along with the DNA, which will also accumulate along the upper layer in the
Living organisms undergo chemical reactions with the help of unique proteins known as enzymes. Enzymes significantly assist in these processes by accelerating the rate of reaction in order to maintain life in the organism. Without enzymes, an organism would not be able to survive as long, because its chemical reactions would be too slow to prolong life. The properties and functions of enzymes during chemical reactions can help analyze the activity of the specific enzyme catalase, which can be found in bovine liver and yeast. Our hypothesis regarding enzyme activity is that the aspects of biology and environmental factors contribute to the different enzyme activities between bovine liver and yeast.
As the solution pH can influence the stability of NaClO-NH3 blend and the elimination of SO2, NOx, the impact of the pH of NaClO-NH3 blend solution on the instantaneous removal as well as the duration time was investigated, and the final pH after reaction was also detected and shown in Fig. 5. It can be seen that the variation of solution pH has a negligible effect on the desulfurization, but the elevated pH has a great promotion on the NOx removal, the efficiencies are significantly increased from 36% to 99% for NO2 in the pH range of 5–12 and from 19% to 65% for NO when the pH is between 5 and 10, after where, both of them are constant. Hence, the optimal pH of the NaClO-NH3 solution for the
in the sample in to many identical samples. The DNA retrieved from the reaction can then be
Both some of the original flies and some offspring died during the experiment which could have influenced the results. If a vestigial fly died before it could mate that would give the wild type males an advantage in mating and producing a greater number of offspring. This would also be the case if any flies were harmed at any time during the mating period. To try and combat this problem the next time this experiment is done a larger number of male fruit flies could be used or more samples taken to try and reduce extremes in the data. Studying fruit fly mating behavior is very important because their generation length is so short and reproduction happens rapidly.
I would suggest to students performing the nitration to make sure their benzoic acid product is very fine and broken up before reacting it, as it has a tendency to clump together when it dries and thus proves very difficult to react in solution. I would also suggest keeping a very close eye on the temperature when adding the sulfuric/nitric acid mixture dropwise, as the reaction has a tendency to spike in temperature
This would produce 100% of the dominant phenotype in females with 50% carrying the recessive trait.
These six samples (crude -/+, broken -/+, and whole -/+) were spun at 5000 rpm, and the resulting pellets were isolated and resuspended in DNase buffer. The set of suspensions labeled with a (+) was incubated in DNase enzyme for 15 minutes, and afterwards incubated in 15 uL of STOP solution. All six samples were lysed for DNA extraction with DNA extraction buffer, and micro-centrifuged at maximum speed. To precipitate the extracted DNA, the supernatants from each of the six samples were added to their correspondingly labeled micro-centrifuge tubes containing 7% ethanol (Parent et. al, 2008To bind the DNA, the ethanol lysate mixtures were transferred to labeled spin columns and spun for one minute in the micro-centrifuge at maximum speed. To wash the bound DNA, the spin columns were washed and spun three times at maximum speed. In order to elute the bound DNA, the samples were washed in 80 uL of distilled water and spun again for 2 minutes at maximum speed (Parent et. al,
An individual can be homozygous dominant (two dominant alleles, AA), homozygous recessive (two recessive alleles, aa), or heterozygous (one dominant and one recessive allele, Aa). There were two particular crosses that took place in this experiment. The first cross-performed was Ebony Bodies versus Vestigle Wings, where Long wings are dominant over short wings and normal bodies are dominant over black bodies. The other cross that was performed was White versus Wild where red eyes in fruit flies are dominant over white eyes. The purpose of the first experiment, Ebony vs. Vestigle was to see how many of the offspring had normal bodies and normal wings, normal bodies and vestigle wings, ebony bodies and normal wings, and ebony body and vestigle wings.
consists of a polyhedral head and a tail. The tail is used to inject DNA into a
In biology class, we were learning about enzymes. Enzymes are proteins that help catalyze chemical reactions in our bodies. In the lab, we were testing the relationship between the enzyme catalase and the rate of a chemical reaction. We predicted that if there was a higher percentage of enzyme concentration, then the rate of chemical reaction would increase or it would take less time. We placed 1 ml of hydrogen peroxide into four depressions. Underneath the first depression, we place 1 ml of 100% catalase and make 50% dilution with 0.5 ml of water. We take 50% of that solution and dilute with 0.5 ml of water and we repeat it two more times. there were four depressions filled with catalase: 100%, 50%, 25% , 12.5 % with the last three diluted
...et light. If the LAA plate glows green under exposure to ultraviolet light, then we can conclude that our unknown insert piece of DNA would be the kan gene. If it does not glow green under exposure to ultraviolet light, then then we streak the colony from our LAA plate onto the LAC plate using a sterile glass spreader. When the LAC plate is dray, we place it upside down in the microfuge rack so that it can be incubated at 37 ºC. Incubation at 37 ºC will allow the transformed bacterial cells to grow. If we see bacterial growth on the LA plate containing chloramphenicol, we can conclude that our unknown insert piece of DNA would be the cat gene, since the cat gene is resistant to chloramphenicol. Afterwards, we then grab the microfuge tube labeled NP and repeat the aforementioned steps shown above pertaining to the LA plates. This would be considered our control.
In this lab, it was determined how the rate of an enzyme-catalyzed reaction is affected by physical factors such as enzyme concentration, temperature, and substrate concentration affect. The question of what factors influence enzyme activity can be answered by the results of peroxidase activity and its relation to temperature and whether or not hydroxylamine causes a reaction change with enzyme activity. An enzyme is a protein produced by a living organism that serves as a biological catalyst. A catalyst is a substance that speeds up the rate of a chemical reaction and does so by lowering the activation energy of a reaction. With that energy reactants are brought together so that products can be formed.
The idea of the project was to experiment breeding Drosophila Melanogaster (fruit fly) to figure out if certain genes of that species were sex linked or not (autosomal). A mono-hybrid cross and di-hybrid cross was performed. For the mono-hybrid cross, white eyed female and red eyed male were placed in one vial for them to reproduce. For the di-hybrid cross, red eyed and normal winged flies and sepia eyed and vestigial winged flies were placed in their vial to reproduce. In the mono-hybrid cross the results expected were within a 1:1:1:1 ratio. Expected results similar to the expected desired null hypothesis proposed with what the F1 parental generation breeds. The potential results would have had to have been within the ratios of 9:3:3:1. The results were clear and allowed the null hypothesis to be correct. The white eyed gene in the fruit flies is sex linked. Sepia eyes and vestigial wings are not sex linked and are examples of independent assortment.
A fly sample was captured twice a day at noon and sunset to determine if any of these conditions were relevant. The conditions under observation consisted of human presence, wood smoke from a fire inside or outside, open windows, and simulated cattle or human odor (Vale et al. 2013). The total catches of flies were unaffected by all of these conditions. However, the rise in ambient temperatures had a significant increase in fly abundance. During the hottest months of the year, the amount of fly catches increased from 5%-13% (Vale et al. 2013). Another interesting finding was that 62% of the flies caught were female (Vale et al. 2013), the sex of tsetse flies that less commonly attack humans. Some of the flies captured were old enough to be potential vectors of sleeping sickness (Vale et al.
The restriction enzymes SmaI cuts DNA vertically. This results in two DNA fragments with blunt ends. Next, the gene is spliced into a vect... ... middle of paper ... ... le by stopping illness but this process has also been vandalised for many uses which are not necessary.