Type your CER for Experiments 8A and 8B in the space below, following these directions. Claim: The substances in Experiment 8 A and B were both mixtures that we were able to separate by using physical methods of separation. Evidence: Experiment A: In the first step of this procedure we put 1.5 grams of sulfur, a yellow grainy substance into a test tube and added 5 grams of clear water. We then stoppered the test tube and shook the sulfur and water substance together. Once the sulfur and water was shaken thoroughly, it was clear that the two substances were a heterogeneous mixture. There were still visible bits of the yellow grain substance in the clear liquid. We then poured the contents of the test tube (the sulfur and water) into another …show more content…
We know this because the original substance which was a black liquid was separated by filtering, and was proved to be a mixture because when we looked at the chromatograph paper after “filtering it” we saw the blue, yellow, and pink pigments that made up the green/black liquid. We started out by pouring the black ink into a filter and the substances were not separated. Because filtering the black liquid didn’t separate the substance, we then used chromatography paper to separate the ink. Chromatography paper is a method used to separate colored substances. We put a small line of black ink on the paper then put the tip in the water and let the ink slowly separate. The black ink was proved to be a mixture when we came in the next day and you could see the yellow and pink and blue in that came from the black line of ink. Then when we cut up the pieces and them in water and the water turned green which proved that those three colors made up the dark green/black ink. In conclusion, we can tell that the black ink was a mixture because the chromatography paper showed all the different types of substances that made the mixture, and we know it was separated by physical means because the chromatography paper was acting like a
The inks are not pure substances since the pigments get separated along the filter paper.
To separate molecules that are attracted to one another using the principles of intermolecular forces as well as a scientific ratio to find the identity of unknown substances.
Moreover, another purpose was to learn how to use different lab techniques, such as filtration and proper heating of test tubes, in order to determine the mass of products produced by the reaction. Not only that, the experiment demonstrates how transition elements can often form more than one type of reaction due to their differently charged ions, like Fe.
Chromatography is a method to distinguish between organic and inorganic compounds so that they can be analyzed and examined. By performing analysis of a compound, a scientist can figure out what makes up the compound. Chromatography related techniques have been used for centuries to separate materials such as colorants extracted from plants. However, Chromatography was first developed in 1900 by Russian scientist Michael Tswett. He continued
The material and equipment used was a sample of candy such as M&M’s, skittles, and Reese’s pieces. Set food colors for comparison. Filter paper or coffee filters. 0.1% salt solution {1/8 tsp salt in 3 cups of water}. Clear plastic 9 oz cups. Blow dryer. Also you will need some toothpicks and small {1 oz} plastic cups. This are the materials and equipment we used for this experiment .the objective of the experiment is to use the technique of paper chromatography to show that it can be used to separate from each other in a mixture. To understand the principles of paper chromatography.
By taking a sample size of 5, while still not particularly large it decreased the effect of random errors as an average was able to be calculated. Another strength was using the same size test tubes throughout the experiment. This made sure the surface area and exposure to oxygen in the air was kept consistent which would allow for more accurate results. A weakness of this experiment was that it was difficult to distinguish the exact point when the methylene blue had disappeared. As the top part of the test tube was exposed to oxygen in the air, the milk solution never went completely white and there was a blue ring on top of each test tube which stayed blue. It was also difficult to put the exact same amount of methylene blue into each test tube because you are not actually measuring it but rather just placing drops into the test tubes which is a little difficult to
Experiment one was conducted to show the separation of plant pigments using a process called paper chromatography. The significance of this lab was to show different types of pigments and discover which pigments would have the highest band along the filter paper. Experiment one of the lab exhibited that chlorophyll a (figure 1) would display the highest band of pigments on the filter paper when using the paper chromatography process rather than chlorophyll b, xanthophyll and carotenoids showing higher bands of pigments.
A line was drawn approximently one centimeter from the triangle. This line represented the “start line” for the experiment. Using the mortar and pestle, a large piece of spinach and 5 ml of 90% isopropyl alcohol was grinded until a thick liquid was created. Chromatography solvent (90% Isopropyl Alcohol) was then placed into a Microcentrifuge tube and eight drops was transferd onto the line of the chromatography paper using the capillary tube. One Centimeter of the chromatography solvent was added to the chromatography jar and the chromatography paper was placed in the jar so the tip of the triangle dipped into the solvent. After 15 minuets the chromatography paper was observed and the results were recorded. When the solvent line reached approximately one centimeter from the top of the chromatography paper, the paper was removed and observations of the furthest point of the solvents progress was recorded before the line
For the lab experiment of mixing the diffusion and exposure solution, we are going to test if the pigment is released in the exposure solution. We used materials as follows: Obtaining a beet, we punched out 2 cm long beets using a cork borer. Then we wash the beets in running water, after that we prepared 10 test tubes each containing different pH solution.
The detergent made the two solutions mix because part of the detergent’s molecule is polar and the other part is non polar. So, test tube 1 mixed with the polar part of the detergent and test tube 4 mixed with the non-polar part of the detergent, which allowed both test tubes to mix together.
mixture and then the mixture was boiled for a further period until it was concentrated
Chromatography and electrophoresis are used for many wonderful processes that take place in labs. Chromatography and electrophoresis are separation techniques employed by chemists. Chemists execute multiple chemical tasks daily therefore they must find the most efficient ways to carry out these functions. Even though electrophoresis has some benefits, Chromatography is more useful than electrophoresis because it is more versatile and can be widely used in research; chromatography should be used more than electrophoresis in scientific research because it is more effective in a lab setting and can be used in more fields.
The objective of the experiment Fractional Distillation and Gas Chromatography was to determine the organic liquids existent in 30 mL of an unknown mixture. This can be accomplished by fractional distillation. Distillation is a technique that allows one to separate liquids by boiling the liquids and which removes water from its impurities. Simple Distillation separates out a liquid/solid mixture. If the boiling point is greater than 40 degrees Celsius then simple distillation will be able to be used to separate two liquids, if not then Simple Distillation cannot be used. Fractional distillation splits two liquids and transfers them to their pure form by using their boiling points. In order for Fractional Distillation to be used, the boiling point has to be less than 40 degrees Celsius. The only difference between them is that in fractional distillation a column runs to the flask while in simple distillation there is no column. Of the two main types of distillation, Simple and Fractional, Fractional distillation is the one that is used to split the liquids. To get this experiment unde...
Results for part A: when the copper carbonate is heated it produced a gas called copper dioxide the gas flows into the limewater. The limewater becomes cloudy and begins to bubble due to this the limewater becomes white and milky the limewater’s test tube also begins to heat up. When lifted up the test tube is clear at the bottom, white and murky at the top.
A mixture is a combination of two or more substances that do not have fixed proportions to one another. The two types of mixtures that exist are heterogeneous and homogeneous mixtures. A heterogeneous mixture is a mixture where someone is able to see more than one color or type of matter, This mixture has more than one phase and a non-uniform composition. A homogeneous mixture, however, is a mixture that looks the same throughout the composition, and has one phase and uniform composition (Experiment 1.4: Separation of MIxtures Student Instruction Sheet). Separating the substances in mixtures can be done using different methods, including decant, filtration, evaporation, distillation, magnetic separation, and chromatography.