E Coi Experiment

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In this experiment, the most studied bacterium, Escherichia Coli, is utilized as a model bacterium for genetic transformation. E. Coli is a model bacterium since it can extensively be studied to understand a specific phenomenon. The benefits of E. Coli refer back to the observation that it can survive varied conditions; is a single-celled bacterium; is able to rapidly reproduce and grow; most E Coli strains are harmless; E Coli can easily be genetically transformed. Diving more in depth in this experiment, genetic transformation can occur through projectile bombardment, electroporation or heat shock. Genetic transformation requires the availability of technology in order for a cell to take up and express a foreign DNA, from another organism …show more content…

(GFP) is integrated into a plasmid with a gene resistant to an antibiotic (ampicillin). Ampicillin- resistance is included in determining if the E. Coli survived and grew. Ampicillin is an antibiotic that kills bacteria; ampicillin – resistance is applied to observe those cells that took up the plasmid and will survive and grow. As for bacteria without plasmid and resistance, is unable to grow and survive, since the antibiotic prevents E Coli from constructing a cell wall. Our control group will be the sample with no ampicillin, to compare bacterial growth. The purpose of this experiment is to observe if we can control E. Coli in order for it to glow, and to where it can become resistant to ampicillin through the insertion of a gene that it normally doesn 't acquire. Testing where and in which plate would undergo a genetic transformation. As mentioned above, genetic transformation is when a cell is obtaining and portraying a form of DNA that is not its own. Genetic transformation was discovered in Streptococcus pneumoniae, by Griffith in 1928. (Lorenz, M G, and W. Wackernagel) Genetic transformation is significant as it is typically used in the agricultural sector, in the field of bioremediation, and in the field of medicine …show more content…

Coli can evidently be manipulated to express the foreign DNA as we can observe in (figures A, B, and C), through the agar plates of –pGLO/ LB, -pGLO/ LB/ AMP, +pGLO/ LB/ AMP, and +pGLO/LB/AMP/ARA. These results contribute to support our stated hypothesis that the sample –pGLO/ LB would grow the most bacteria, - pGLO/ LB/ AMP would not have bacterial growth, +pGLO/ LB/ AMP would have some bacterial growth, and +pGLO/LB/AMP/ARA would be the only bacterial glowing under UV light.
- pGLO/ LB/ AMP did not have bacterial growth because it did not contain ampicillin; in which ampicillin is an antibiotic meant to kill bacteria, like E. Coli. - pGLO/ LB/Amp presence of ampicillin, which destroyed the cells cell wall and prevented growth. –pGLO/ LB had the most growth and survived because there was no ampicillin to destroy the sample. The sample containing +pGLO/ LB/ AMP had some bacterial growth in consequence of pGLO and plasmid DNA aggregation. All in all, the petri dish with the sample +pGLO/LB/AMP/ARA, was triggered by arabinose who activated the fluorescent gene, making the bacteria express glowing under UV

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