The aim of this experiment is to test two different concentrations of Dettol disinfectant in limiting soil (sand) bacteria growth.
Hypothesis-
I think that the concentrations with the most Dettol disinfectant will stop bacterial growth the most. Out of all the percentages I think the 100% disinfectant will stop the bacteria the most, this solution would be made from 10ml of Dettol disinfectant and 0ml of water.
Variables-
The independent variable in this experiment are the amounts of Dettol disinfectant used for each petri dish, the dependant variables are the measurement of bacterial growth that grows during the time and the controlled variables are everything we kept the same such as the amounts of agar solution, the beaker size, same sized petri dishes, same soil and same sized filter papers.
Equipment-
• Dettol disinfectant
• Measuring cylinders
• Beakers
• Labels
• Soil
• Agar
• Petri Dishes
• Filter paper
• Sticky tape
Method-
1- Collect the Dettol disinfectant and water quantities based on the concentration you were given to make. Use 10Ml measuring cylinders.
2- Prepare your disinfectant concentrations into 100mL beakers.
3- Prepare soil and water together allowing soil to settle at the bottom, the pour 10mL of this water without getting any of the soil into the beaker, get rid of the remaining soil and water.
4- Collect a beaker of 50mL full of agar from your teacher and then mix the 10mL of soil water with the agar carefully.
5- Pour the agar/soil water into the petri dishes evenly.
6- Allow the agar to cool and set.
7- Soak your small disk of filter paper into the disinfectant concentration, let it soak for about 10 minutes.
8- Once soaked, place the filter paper into the middle of the ag...
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...e starting the experiment, being very carful with all the measurements and trying your best to keep everything that’s meant to be the same all equal to everyone else’s. If your working in groups the aim would be to get similar results without having large different between the few otherwise if your results are not close or similar that usually means something went wrong along the way. The results ended up consistent to what most of us thought would happen with the bacterial growth.
Conclusion-
In conclusion my hypothesis was correct towards this experiment, the concentration with the most Dettol disinfectant had the least bacterial growth and the least Dettol disinfectant had the most bacterial growth occurring in our agar plates, the experiment was successful to show the soil bacterial growth and how well the Dettol disinfectant works to prevent the growth.
Each subsequent trial will use one gram more. 2.Put baking soda into reaction vessel. 3.Measure 40 mL vinegar. 4.Completely fill 1000 mL graduated cylinder with water.
Place a clean, dry 125 mL Erlenmeyer flask on balance, and slowly dispense liquid bleach until there is about .5 g. Record the mass of bleach, and add 25 mL of de-ionized water and about 2 g of KI. Swirl contents until the KI dissolves. Then add 3 drops of 1 M H2SO4, mix, and let stand for 1 or 2 minutes.
Mix 50ml of WATER with 15ml (1 tbsp) of SALT in one beaker or cup
3. Use a syringe to collect 3.0 mL ethanol (EtOH). Twist the syringe so that it attaches to the two-way valve. Wait until the TA instructs you to put the flask into the water bath.
· Rinse out mixture in flask and leave water running to get rid of the
Planning Firstly here is a list of equipment I used. Boiling tubes Weighing scales Knife Paper towels 100% solution 0% solution (distilled water) measuring beakers potato chips Cork borer. We planned to start our experiment by doing some preliminary work. We planned to set up our experiment in the following way.
The input variables are the ones that I can change in order to affect the experiment and the outcome variables are the ones I will measure to see how the input variable has affected it. Input Variables --------------- Amount of calcium carbonate Amount of hydrochloric acid Surface area of calcium carbonate Concentration of hydrochloric acid Temperature of hydrochloric acid Introduction of a catalyst Outcome variables ----------------- Amount of calcium chloride released Amount of water released Amount of carbon dioxide released Change in weight
Plan 1. Collect 4 different sized beakers 2. Boil some water in the kettle 3. Pour 50ml into each beaker 4. After 1 minute check temperature 5.
Rinse your beaker thoroughly to wash any excess powder. 12. Repeat steps 7-11 3 more times for reliability. To make sure the temperature still stays hot by continue heating the water a little bit using the hot plate. 13.
3.) Divide your 30g of white substance into the 4 test tubes evenly. You should put 7.5g into each test tube along with the water.
In a 100ml beaker place 50mls of water, measure the temperature of the water and record this initial temperature onto a table. Set the timer and add one teaspoon of Ammonium Nitrate to the water, stir this continuously until the Ammonium Nitrate has dissolved.
I chose to do the effects of different disinfectants on bacteria because I have always wondered if disinfectants such as Clorox, Lysol, and hand sanitizer actually kill 99.9 percent of germs. Also, I know that there are harsh chemicals that can be damaging to us over time. Do these harsh chemicals get the job done? Maybe taking a green, organic way could be the solution in the future. My main question is which is the better disinfectant, Clorox Disinfecting Wipes or Lysol Disinfecting Wipes. I want to uncover the truth about each product and show the reality of both. Are they really getting the job done?
About 25ml of distilled water was added. Then 2-3 drops of phenolphthalein indicator solution were put in. It was swirl to dissolved the solid.
3rd create wells: put a comb template in middle of the tray; wait until the mixture becomes solid. After,
The duration of the experiment should be increased as the thermal death times of B. subtilis at 60, 70 and 80°C were unable to be determined within 110 minutes. The duration can be increased to 180 minutes so as to better investigate its thermal death times. If the presence of bacterial growth was still observed after 180 minutes of exposure, it can be assumed that B. subtilis is able to survive well in that temperature. An exposure time of one day can be carried out to confirm this assumption.