Affect of the Rate of Reaction of Amylase on Starch and How Its Affected by the Concentration of the Substrate

Affect of the Rate of Reaction of Amylase on Starch and How Its Affected by the Concentration of the Substrate

Equipment list

Ø Test tubes

Ø Measuring cylinder

Ø Thermometer

Ø 3 pipettes

Ø Starch solution

Ø Amylase

Ø Iodine solution

Ø Water

Ø Water bath

Ø Spotting tile

Ø A stop watch

Ø Goggles

Ø Beakers

Health and safety

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Remove all hazardous jewellery such as dangling earrings, bracelets,

and chains.

Make sure items of clothing cannot distract you such as ties and

scarves, also

Make sure all long hair is tied back. Wear eye protection even for the

most dilute solution. If any solution gets into the eye then the

effected area is to be washed in cold water immediately. The person

conducting the experiment should also try to wear protective gloves as

iodine; one of the substances used in this experiment is an irritant.

If any solution or iodine is spilt on to the skin then the person

affected should wash with clean water. If there are any reactions or

any unusual occurrences then you should report to your g.p.. If there

are any spillages they should be cleaned away in an adequate way. This

will prevent accidents in lab rooms.

Method

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Ø Place all needed equipment as shown in the diagram

Ø Make a starch solution which consists of 4 ml of starch and 6 ml of

water in a test tube

Ø Check the temperature of the water in the water bath with a

thermometer that should be at 37°c, approximate body temperature. The

temperature should be at 37°c as this is an optimum temperature range

and if temperatures exceed this range too much the enzymes become

denatured. This is because the enzyme loses its shape and gradually

stops working.

When the water in the water bath is at the required temperature of

37°c then

Ø Add 2ml of amylase enzyme into the solution that consists of water