Activity of Diastase on Starch at Different Temperatures at PH5.8

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Activity of Diastase on Starch at Different Temperatures at PH5.8 Temperatures - 25,40,60&100 Procedure - I will label 5 test tubes at different temperatures as stated above leave one at room temp as control I will place each test tube in appropriate water bath for 5mins exactly. Remove and cool rapidly to room temperature. Requirements 5 test tubes labelled. Test tube rack Thermometer Pipette (5cm) Stop timer White tile 6 glass rods. Water baths maintained at 25, 40, 60, &100 25cm of 0.5% of Diastase solution. Starch solution as substrate 25cm of 0.5% dilute iodine. Enzymes are proteins that control vital biological processes. They often act as biological catalysts. They are made in cells. A catalyst is a chemical substance which speeds up a reaction but does not get used up during it. One enzyme molecule may be used several times over. A rise in temperature increases the rate of most chemical reactions and a fall in temperature will slow them down. In many cases a rise in 10¦ C will double the rate of reaction in acell. This is particle theory. I am going to investigate the temperature at which reactions occur. An increase in temperature will result in a n increase in kinetic energy. Since the kinetic energy increases, the velocity of particles will also increase. Since the speed of particles increases, they should collide more often and therefore the speed of reaction increases. The particles will also have more energy thereby speeding up the reaction even more. It has been suggested that for every 10degree rise in temperature, the speed of the reaction will double till optimum. At low temperatures particles of reacting substances do not have much energy. However, when the substances are heated, the particles take in energy. This causes them to move faster and collide more often. The collisions have more energy, so more of them are successful. Therefore the rate of reaction increases. The more successful the collisions are

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