Carb Cutter Lab Report

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Recommended: Amylase on the rate of starch digestion

According to Authority Nutrition, Carb Cutters block 50-65% of carb-digesting enzymes.(“What Are Carb Blockers”). How accurate is this statistic? In a recent study I participated in, I studied whether Carb Cutter prevents amylase from digesting starch. Carb Cutter is used to block starch from digesting in the body. Normally, starch is broken down by the enzyme amylase into glucose. However, when Carb Cutter is used, it prevents the function of the enzyme amylase, causing starch not to be digested into glucose. Many times Carb Cutters are used to help with weight loss since it is claimed to stop starch from being digested. However, there may be some suspicion on whether or not Carb Cutters actually work or if they are just a fraud. My experiment studied whether or not Carb Cutters actually work and how effective they really are. …show more content…

To begin the study, I first calculated how much of each solution I would need. I knew that the final volume of my reaction solution needed to me 30ml, so I calculated how much of starch, amylase, and tris buffer I would need. I used the formula Concentration (initial stock solution) x Volume (initial stock solution)= Concentration (final solution) x Volume (final solution). Using this formula, I found that I would need an initial concentration of 21 ml of starch, 1 ml of amylase, and 8 ml of the tris buffer. After calculating the amounts of substances I would need, I created two different solutions, one with the Carb Cutter and one without. Carb Cutter claims to block starch, however, to find this I needed to test the absorbance level of the control to compare the effect Carb Cutter had on the solution. Below is a graph showing the concentration of the control reaction over one minute intervals through the

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