Supplementing Camel's Milk

GC analysis

The Gas Chromatography GC was used a Agilent Technologies (7890A) interfaced with a mass-selective detector (MSD, Agilent 7000 Triple Quad) equipped with a polar Agilent HP-5ms (5%-phenyl methyl poly siloxane) (J&W Scientific, Folsom, CA, USA) equipped with a capillary column (30 m × 0.25 mm i. d. and 0.25 μm film thickness) and FID detector. Medium (Country) used for fractionations the date fruits, Echinacea extracts and camel according to methods performed by Dionex 22.

GC-MS analysis

Ethanol extracts of Echinacea arial part or date pulp fruits were performed separately using a Perkin-Elmer GC Clarus 500 system comprising an AOC-20i auto-sampler and a Gas Chromatograph interfaced to a Mass Spectrometer (GC-MS) equipped with a Elite-5MS (5% diphenyl/95% dimethyl poly siloxane) fused a capillary column (30 × 0.25 μm ID × 0.25 μm df). For GC-MS detection, an electron ionization system was operated in electron impact mode with ionization energy of 70 eV. Helium gas (99.999%) was used as a carrier gas at a constant flow rate of 1 ml/min, and samples were injected with a split ratio of 50:1; helium was used as carrier gas at 1.0 mL/ min. The column temperature was maintained at 100°C for 1 min after injection then increased at 10°C /min to 275°C which was sustained for 20 min. The time required for chromatography of one sample was 40 min.

TIC method for Identifications of the constituents

Identification and quantification of constituents: The relative percentage amount of each component was calculated by comparing its average peak area to the total areas (TIC) by apparatus software. The mass-detector used in this analysis was Turbo-Mass Gold-Perkin-Elmer, and the software adopted to handle mass spectra and chromatograms...

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...don Scientific, and Cheshire, UK), sectioned (4μm) and stained with hematoxylin and eosin (H&E) stain. The slides were mounted with neutral balsam.

Sensory evaluation

The Sensory evaluation of camel's milk, date pulp and Echinacea mixtures were assessed by regular taste panel (appearance,5-body& texture,5-and flavor ,10) by the staff-members at Food Sci. Dept., Fac. of Agric., Ain Shams University according to Clark et al., 26.

Works Cited

46. Al-Farsi M., Alasalvar C., Al-Abid M, et al.: Composition and functional chracteristics of dates, syrups and their by products. Food Chem 2007; 104:943-947

47. Chaplin D.D.: Overview of the immune response, J Allergy Clin Immunol 2010;125(2), S3-23.

48. Iemoli E, Piconi S, Fusi A, et al.: Immunological Effects of Omalizumab in Chronic Urticaria. A Case Report. J. Investig Allergol Clin Immunol 2010; 20(3): 252-254.