Metabolism Lab Report

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Introduction
Multicellular organisms continue to undergo many life sustaining processes. Metabolism is described as the aggregate total of chemical reactions occurring in an organism. Metabolic processes and can be categorized as either anabolic, synthesizing complex molecules, or catabolic, breaking down complex molecules. Both catabolic and anabolic processes require the use of energy in the form of adenosine triphosphate, or ATP. As catabolic reactions break down molecules, energy is released as bonds are broken to disrupt the molecules. Anabolic reactions synthesize reactions, thus require an input of energy to form these bonds. The input and release of energy comes from aerobic respiration, which converts oxygen and glucose into carbon …show more content…

When placed in the controlled environment of ambient water, they exhibited normal behavior, swimming at moderate speeds, not making sharp turns, or hitting the enclosed system. They consumed 232 milligrams of oxygen per liter per hour per kilogram of fish. Under the 17˚C water conditions, the orange fish had rapid movements where it would repeatedly hit the top of the closed system. They consumed 77 milligrams of oxygen per liter per hour per kilogram of fish.
In the second trial, the fishes together weighed 7.22 grams. When placed in the ambient water, the fishes exhibited normal behavior. They consumed 199 milligrams of oxygen per liter per hour per kilogram of fish. Under the 18˚C water conditions, both fishes exhibited moderate, normal movement. They consumed 150 milligrams of oxygen per liter per hour per kilogram of fish.
The mean oxygen consumption rate per kilogram for the controlled experiments was 325 milligrams of oxygen per liter per hour per kilogram of fish and the mean for the experimental experiments was 19 milligrams of oxygen consumed per liter per hour per kilogram of fish. With a total of 6 trials, the t-test value was 1.6179 and the p-value obtained was 0.165. However, excluding trials with positive slopes, with 3 trials, the t-test value was 1.2649, the p-value obtained was 0.296, the mean was 587 milligrams of oxygen consumed per liter per hour per kilogram in the controlled experiment, and the …show more content…

If the p-values were below a 0.05 significance level, then the data will be considered statistically significant. However, the t-test value of 1.6179 and a p-value of 0.165 for 6 trials, the t-test indicates that the null hypothesis is retained and can conclude that there is no effect on Carassius auratus metabolism when temperatures are lowered. In addition, excluding trials with positive slopes gives a t-value of 1.2649 and a p-value of 0.296 for 3 trials, still indicating that the null hypothesis is retained. The trials with positive slopes were excluded because respiration requires the depletion of oxygen, not the creation of it as a product. The Q10 value, a quantity that measures an organism’s physiological temperature sensitivity due to an increase of 10˚C, demonstrated a change in metabolic rate due to change in temperature with values of 3.97 and 1.50, especially since the values are greater than 1. A Q10 of nearly 4 indicated that when temperature increased by 10˚C, the metabolic rate would increase four-fold. In the scenario of the second trial, the metabolic rate increased by a factor of

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