Investigating the Relationship Between the Transpiration Rate of a Shoot and the Degree of Opening of the Stomata of Its Leaves

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Investigating the Relationship Between the Transpiration Rate of a Shoot and the Degree of Opening of the Stomata of Its Leaves

I will be investigating the relationship between the transpiration

rate of a shoot and the degree of opening of the stomata of its

leaves.

Transpiration is the loss of water vapour from the surfaces of a

plant. Solar energy turns the water in the plants into a vapour

causing it to evaporate into the leafÂ’s internal air spaces before

diffusing out of the stomata into the air. The water is able to

evaporate out of the leaf as the leaf has a high water potential and

the surrounding has a low water potential. The water molecules pass

down the concentration gradient from the spongy and palisade mesophyll

cells into the leafÂ’s internal air spaces before diffusing out into

the air.

For this experiment, I will need to vary a factor that affects both

the transpiration rate and the degree of stomata opening in order to

determine the relationship. Factors that affect transpiration rate

are humidity, temperature, light intensity, water supply, plant

surface area, plant species and wind speed. These in fact affect both

incidents because transpiration rate depends upon stomata opening to

allow gas exchange. Using the apparatus available in a school

laboratory, I will determine the relationship by varying the wind

speeds, hence, keeping all the other variables mentioned above

constant. The reason for choosing wind speed is because none of the

other factors can be kept mutually variable for reasonable results

(i.e. if light intensity is chosen as a variable, the temperature will

fluctuate as well).

In the control test where there will be no wind, water vapour will be

able to build up in the air spaces of the leaf and form a layer around

the leaf as water transpires out. This will reduce the water

potential gradient between the inside and outside of the leaf. This

will in turn reduce the rate of transpiration.

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