The objective of this lab is to test enzyme productivity, whilst evaluating the factors that influence enzyme catalyzation activities and recording the results. The intent was to discover how temperature impacted the enzymes efficiency. Introduction: Enzymes function to catalyze a change in a chemical reaction they are normally made of proteins and can be used repeatedly. The enzyme studied in this experiment is catalase an enzyme known for breaking down hydrogen peroxide. Enzymes are not always able to accomplish what they are designed to accomplish, they can become denatured (lose shape) due to temperatures, pH, inhibition, competitive inhibition, etc. Enzyme efficiency or activity can be affected by external factors, in this experiment …show more content…
Next, place one tube in a warmer that is set to 37C and leave the tube to reach this temp. For the 4th tube you will place this tube in a special warmer that is keeping the water at a constant 55C allow tube contents time to reach temp. Step 3: Take a break until the test tubes have been in temperature baths for 10 minutes. To identify the enzymes activity, take tube 1 out of the ice bath it is in. Add ~10 drops of the catalase solution to the mixture. Now, pour the contents of test tube into a Nalgene bottle capable of holding 250mL and mix contents. Place an O2 sensor in the mouth of the Nalgene bottle thus plugging the bottle ensuring no outside air will contaminate the air the probe/sensor is reading from. One can start data collection after 30 seconds, collect for 3 minutes, retrieve a graph of the O2 data on a time-longitudinal scale disconnect O2 sensor from Nalgene bottle, pour out contents into a safe and disposable waste receptacle/drain. Clean out Nalgene bottle and thoroughly dry it as well as possible. Calculate the slope of the reaction or the % of change of O2 to calculate the reaction rate. Step 5: Using the tube at room temperature (23C) repeat step 3 for this tube and
We then took 1ml of the 0.1% solution from test tube 2 using the glucose pipette and added it to test tube 3, we then used the H2O pipette and added 9ml of H2O into test tube 3 creating 10ml of 0.01% solution.
Repeat for each trial. Rinse volumetric pipette with vinegar and drain into the waste beaker. Weigh and record the mass of each 200mL beaker. Add 10.00mL of vinegar into each beaker and weigh them and record their again. Add 50mL of de-ionized water to the beakers and place them under the drop counter on top of a stir plate, submerging the pH meter into the solution. Place the stir bar into the beaker and carefully turn on the stir plate so that the stir bar spins without splashing or hitting the sides of the beaker or the pH
3. Drop tablet of Alka-Seltzer into the cold beaker of water and time length of reaction with a stopwatch
The purpose of this experiment was to discover the specificity of the enzyme lactase to a spec...
3. Fill up the 50 milliliter beakers all the way up with deionized water. After, use your scopula to scoop out a small portion of one substance and put in into your beaker. Then put your hot plate onto medium temperature and rest the mixture onto the plate.
6. Place the test tube in the beaker. Secure the test tube and thermometer to the retort stand using clamps. Begin heating the water bath gently.
Then add growth media until the sample is submerged beneath the 2-3 ml of liquid, cap the tube and invert several times to mix thoroughly, incubate the tube while shaking vigorously in a shaking incubator at 250 rpm for a couple hours, then allow the sample to sit.
Then we pulse-spinned the contents for allowing flow to the bottom. Following, we utilized a floating rack to allow incubation for thirty minutes. After incubation, we proceeded to load 2 micro liters of loading dye into each individual test tube. Following, we allowed the samples to rest on ice. Since we utilized agarose gel, at this point we obtained the gel and filled the chamber with the buffer. The buffer was loaded properly, so we had it properly cover the gel just enough, and it covered both negative and positive poles at the proper height. Next, we loaded ten micro liters in each respective well in the gel. Following, we covered the chamber and connected the leads. Then allowed current to flow for 43 minutes is how long it took our group. The handout recommended approx. 30
The factors that were investigated in the enzyme lab were specificity of substrate, concentration of substrate and enzyme, pH of reaction solution, and temperature of reaction solution.
5.) One at a time, place your test tubes in the water bath and heat the first test tube to 25 , the second to 50 , the third to 75, and the last to 100 degrees c. Remeber to stir with your stirring rod every so often.
13.Repeat steps 1-12 for the 80℉, 95℉, and 110℉ catalase solutions. Use clean filter paper each time you test. Record the times for the three trials in the appropriate column of the data table.
In a 250ml beaker place 100mls of water, measure the temperature of the water and record this initial temperature onto a table. Set the timer and add one teaspoon of Ammonium Nitrate to the water, stir this continuously until the Ammonium Nitrate has dissolved. After 1 minute measure the temperature and record it, do this for a further 2 minutes (3 minutes in total). Repeat this process for a total of 10 teaspoons.
Analyze each fraction by spotting 10 times with capillary tubes on a TLC plate, which is exposed to iodine vapor for 15 minutes.
tube. Add 6 mL of 0.1M HCl to the first test tube, then 0.1M KMnO4 and