Potato Enzyme Lab

It is important that all cells perform chemical reactions at a swift pace. In order for the cells to perform certain reactions rapidly, they must acquire protein catalysts, known as enzymes. An enzyme contains an active site, where the binding of the substrate occurs. Thus, forming an enzyme substrate complex, where substrate molecules are brought together and aligned. Now that the substrate is aligned, the activation energy is reduced and the chemical reaction may continue at a fast pace with a normal body temperature. If an enzyme is not completely used up during a completed chemical reaction, then the enzyme will isolate itself from the product of the substrate and can now freely bind to another substrate. Enzymes control processes by bringing about reactions. If an enzyme loses it’s unique shape or structure, also known as denaturation, the enzyme

The factors that were investigated in the enzyme lab were specificity of substrate, concentration of substrate and enzyme, pH of reaction solution, and temperature of reaction solution.
Hypothesis
Experiment I: Effect of Different Substrates on the Rate of Enzyme Reaction
As the enzymatic activity increases in the presence of substrates of catechol and resourcinol, then the intensity of the color will also increase because enzymes, such as the potato extract, works better with specific substrates, such as catechol and resourcinol.
Experiment II: Effect of Substrate Concentration on the Rate of Enzyme Reaction As the amount of the substrate, catechol, increases and the amount of deionized water decreases, then the intensity of color will also increase because increasing the amount of the substrate will heighten the activity of the fixed amount on an enzyme, taking it a longer time to convert the substrate into the