Cellular membranes are vulnerable under extreme conditions due either to a barrier to diffusion caused by the hot conditions or expanding when it’s too cold due to the hydrogen bonding. To experiment and make observations about the membranes vulnerability beets were used. For the experiment to take place the beets were all cut out using a cork and were the same size for accurate results. The tubes were then placed under different temperatures to observe the visual, qualitative observation and then spectrophotometer was used to find the actual absorbance. The color intensity showed that the tube exposed to the most heat and most cold were both the most intense, but the absorbance showed that the extreme exposure to the cold had the …show more content…
Since cell membranes make up an important part of life it’s important to know how certain factors such as physical stress particularly the temperature effect the membrane and the vulnerability of these membranes. In both extremes of temperature, the membrane is susceptible to the degradation of the membrane due to the expansion of water when freezing or lack of diffusion when there is too much heat. In this experiment beet tissue was used as a replacement for the membrane since it provides betacyanin, a reddish pigment which secretes from the vacuole when it’s under unhealthy conditions. Since the betacyanin is a good indicator of the condition of the beet(cell) it’s used to replicate the cell membrane under these conditions of varying temperatures. I hypothesized that under both the high and low temperature damage would be caused. …show more content…
In Figure 1, the graph is consistently low absorbance and then reaches its peak towards the end when it is exposed to the freezing temperature. While in Figure 1 the graph is consistently low in absorbance and then increases dramatically towards the -10 degree mark for the qualitative graph shows that the first tube is also close to the freezing tube in the color intensity but then it gets low and also peaks at the freezing temperature. The discrepancy between the data can be due to systematic error since all the other data is precise and the quantitative data strengthened the
The mean for the temperatures is 0.116 and the solvents is 20. We predicted the 37 Celsius would be the most absorbed, but it was the -20 Celsius which can be seen in the graph above.
Osmosis Experiment Planning Aim: The main subject that I will be planning to investigate is the effects of a concentrated sucrose solution on potato cells on the basis of the Osmosis theory. Background knowledge: The plant cell and its structure To understand osmosis in detail I will need to explain the plant cell (which is the cell included in the osmosis experiment) and its cell membrane. Below I have a diagram of a plant cell: [IMAGE] Osmosis is about the movement of particles from a higher concentrated solution to a lower concentrated solution to create an ethical balance via a partially or semi permeable cell membrane. Osmosis in simple terms is the exchange of particles between the cytoplasm inside the cell and the solution outside the cell. What makes this exhange is the partially permable cell membrane.
A molecular miracle occurs every time the Wood Frog faces freezing temperatures. At the point where human skin would freeze and the cells collapse resulting in frostbite, this frog’s system uses colligative properties to secrete sugars which support the frog’s cells and keep them from collapsing.
Dialysis tubing is made from regenerated cellulose or cellophane, and is used in clinical circumstances to ensure that molecule have a filtered flow, and that larger solute molecules do not enter the dialysis tubing (Alberts, 2002). Like a cell membrane, dialysis tubing has a semi-permeable membrane, which allows small molecule to permeate through the membrane. Thus, the dialysis tubing mimics the diffusion and osmosis processes of the cell membrane (Alberts, 2002). Although the dialysis tubing has a semi-permeable membrane, which mimics a cell, its structure is different. The me...
Water Potential of Potato Cells Aim: To demonstrate the Water Potential of Potato Cells. Objectives: · To show the water potential of potato cells using various measured concentrations of a sucrose solution and pieces of potato. · To record and analyse data to verify observed results. · The method and procedure was carried out as per instruction sheet. Observations: The experiment shows that the lower the concentration of the sugar solution, in the Petri dish, the mass of the potato increased.
Permeability of Beetroot Cell Membranes. An experiment to investigate how temperature affects the Permeability of beetroot cell membranes. Interpretation of Results:. 0 ° C 20° C 30° C 40° C 55° C 0.24 0.28 0.75 0.79 0.22 0.26 0.35 0.41 0.81 From 0°C to 30°C there is a gradual increase in absorbency which shows that as the temperature increases it is denaturing the cell wall and cell membrane and allowing the beetroot pigment to leek out into the distilled water.
Future research should test a wider ranger of temperatures. In addition to pinpointing an ideal growing temperature, exploring the effect of higher and lower temperatures could test the tentative conclusions from this experiment that membranes are less affected by warmer environments than by cold environments and potentially identify a temperature threshold at which membranes begin to drastically lose their integrity. Repetitions of this experiment should include more trials to increase accuracy and more consistent treatments. Instead of leaving the beet discs in a -5℃ environment for months and the other discs in their environments for two minutes, the beet discs should all be placed in the different temperatures until all of their internal temperatures match that of their
The purpose of this experiment was to examine the cytoskeleton, its role in cellular shape and adhesion; as well as identify the molecules necessary for cytoskeleton function. There were two experiments preformed, one involving RAW cells and the second involving rabbit skeletal muscle cells. The first experiment required the RAW cells to be exposed to different concentrations of the drug cytochalasin D. This particular drug inhibits the polymerization actin, a protein microfilament involved in the cytoskeleton. It provides shape to cell and is involved in cell adhesion. After incubation of the experimental cultures they were examined for changes in cell structure in comparison to the control cells with no exposure to the drug. The latter experiment
Objective- The objective was to determine the effect of salt water on a red onion cell membrane, but we are more focused on the actual cells.
The effect of temperature on the beetroot membranes Aim of the research: The aim of this investigation is to determine what kind of effect will the increasing temperature have on the plasma membrane of a beetroot cell. Introduction The beetroot contains a red pigment that is kept in the cells by the membranes. If the membranes are damaged, the pigment “betalain” will leek out. The amount of pigment that leeks out can be assessed, as “betalain” will colour any water that surrounds the cell.
Cytoskeleton is a structure that help in maintaining cell shape, cell motion, cell division and transport of vesicles. There are three different protein filaments that make up the cytoskeleton, which are microtubules, intermediate filaments and actin filaments. In term of their functions, microtubules are the structure that support the cytoplasm as it is the major components of cilia and flagella. Contain alpha and beta tubulin, however, alpha tubulin is found in the trachea and esophagus. The trachea and esophagus have three major layers, inner layer (mucosa), middle layer (submucosa) and outer layer (muscularis). In this experiment, three groups prepared the slides, however each group did different treatments. Group one and two did antibody staining, group 1 treated their slide with the antibody of alpha tubulin, while group two treated their slide with BSA blocking solution. We are group three treated our slide with hematoxylin and eosin (nuclei stain not an antibody staining). The purpose of this experiment for our group was to see the fine structures of trachea and esophagus. The results of this experiment were as stated in figures 1-8, we could identify the trachea, esophagus and other tissues.
shows how different abiotic stresses result in unique responses from a plant cell wall [4].
== = This experiment is based on the concept of Osmosis. Osmosis is the diffusion of water molecules from a region of high water concentration to a low water concentration through a semi permeable membrane (in this case, the cell potato cell membrane). The cell walls of the potato cells are semi permeable meaning that water molecules (which are small) can fit through but other bigger molecules such as glucose cannot pass through. The water molecules can flow both ways through the membrane, letting molecules both in and out.
Another error that could have occurred, and that would explain what happened to the cells that were put in the solution with .4M, is that onion skin dried out before it was placed in the sucrose water. Also, there could have been a variation between the different onion epidermis cells that were used. Conclusion: The results of the investigation show that the greater the molarity, the more plasmolysed cells will appear. Plasmolyses is the shrinkage of volume of a cell. This is caused by the falling of water concentrations and ultimately results in the contraction of the membrane.
The Cell, the fundamental structural unit of all living organisms. Some cells are complete organisms, such as the unicellular bacteria and protozoa, others, such as nerve, liver, and muscle cells, are specialized components of multicellular organisms. In another words, without cells we wouldn’t be able to live or function correctly. There are Animal Cells and Plant Cells. In Biology class the other day we studied the Animal Cell. We were split into groups of our own and we each picked a different animal cell slide to observe. My group chose the slide,'; Smeared Frog Blood ';.