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Project on paper chromatography
Project on paper chromatography
Project on paper chromatography
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Candy Chromatography Lab
Paper chromatography is the ability to separate specific parts of a mixture, in order to identify its content. There are many forms of chromatography, but paper chromatography tends to work with substances such as dyes, inks, or any colored chemical. In the fields of biology, paper chromatography benefits police that need to test blood. It can also be used by chemists to test substances in their labs. Lastly, it can be used to identify compounds that may be in a plant substance.
The purpose for conducting this candy chromatography lab was to figure out which colors of skittles were not shown, by separating the pigment out. In this lab, the materials needed were four different colored skittles, rubbing alcohol, two coffee filters, two tall glasses or plastic cups, a pencil, ruler, tape, foil or paper plate, table salt, water, four toothpicks or cotton swabs, measuring cups/spoons, and a clean pitcher. The first step in conducting the lab was to cut the coffee filter. We had to cut two, 3 by 9 cm rectangles. Next, I dropped four drops of water onto a piece of foil, and did not allow them to touch. Then, I put one skittle of each color on the drop, and waited for the color to soak in. Then, I threw away the skittles. I then drew a line one cm from the edge of one end of one strip of paper using a pencil. Then, made four pencil dots along the line, 0.5 cm apart. Underneath the dot, I label the color of the candy you will test on that spot, using abbreviations. I used the colors red,
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The red and blue were most visible and were near the purple. Blue and yellow were also seen on the green. The solution line made it to around 55-60%. After a few minutes had gone by waiting to see the results from the alcohol solution, the colors showed up well. It also took about twenty minutes, and the colors showed up
lastly we will add 1ml of 0.0003M DPIP, we made sure it was mixed well so the tube contained the same uniform colour, as soon as the DPIP was mixed we timed the time taken for the blue to fade from the tube. The outcome for the time taken for the colour to disappear was 3 minutes and 40 seconds which gave us the baseline for our experiment. The normal range for this experiment is usually between 2 and 7, if the time taken was under 2 minutes we would of needed to reduce the volume of the enzyme added and if the time taken was over 7 minutes we would of needed to increase the volume of the enzyme, and adjusting the buffer to make sure the volume in the tubes are 7mls for each experiment. As no modifications where made we can move on knowing our enzyme is the correct volume to conduct our
Experiment #3: The purpose of this experiment to test the chromatography of plant pigments the alcohol test strip test will be used.
Chromatography is a method to distinguish between organic and inorganic compounds so that they can be analyzed and examined. By performing analysis of a compound, a scientist can figure out what makes up the compound. Chromatography related techniques have been used for centuries to separate materials such as colorants extracted from plants. However, Chromatography was first developed in 1900 by Russian scientist Michael Tswett. He continued
Another problem with this experiment was determining at which stage the solution turned completely clear. I had a black piece of card, which I held behind the boiling tube to see when the solution turned clear, however this was not very efficient. I think that it would have been better if I had gotten a white piece of card and wrote a black letter on it, then looked through the solution until I could clearly see the letter.
After extracting, filtering, and condensing the blue and pink dyes off of the candies, it can be determined that the Blue candy is FD & C Dye Blue 1 and the Pink candy is a mix of Yellow 5 and Red 40. During the solvent evaporation for the blue dye, it was observed that when concentrated, the transparency of the dye was similar to that of Blue 1. During the paper chromatography of Project 2 Session 1, the blue dye showed on the chromatogram to have the highest spot height. Similar to the paper chromatography today which the blue dye was observed having the higher spot height as well. Figure 3 of the Project 2 In-Lab Report displays Blue 1 of having an Rf value of .729. The blue dye off of the candy, as seen in Figure 2, has an Rf value of .657.
Trial 5 was the last trial performed on the first day of the experiment. In the period between the next lab session, chemiluminescence and luminol were researched, with findings encouraging the use of distilled water as a solvent and hydrogen peroxide or bleach as a reagent, with the reagents also dissolved in the distilled water.
A bar graph was created using Excel and the percent of sugar in each substance was compared. The graph was included in the lab report.
0,74 0,87 1,00 0,49 100... ... middle of paper ... ... some groups had got different leakage of the pigment in the test tubes with water.
The 1% ethanol test showed the lowest out of all ethanol concentration results for the colour pigment as it gave the result of 3. The membrane was disrupted to a small amount, allowing the red pigment to be more
3.) Divide your 30g of white substance into the 4 test tubes evenly. You should put 7.5g into each test tube along with the water.
This company region regarding sugars has built throughout the most recent couple of generations, that was extremely extended by the corporate voracity regarding prepare baked merchandise and the climbing regarding sustenance manifestations. Candy usually are obtainable in a lot of diners because notoriety provides extended. Moreover numerous small business covering absent been set up since only sugars retailers. Solidified yogurt parlors happen to be close to given that ahead of 1800. Several associations commenced publicizing physical effort putting attention individually upon sugars. The treatments helpful to market place treats usually are inside along with out and about diverse based on the interpersonal occasion of folks
The concentrations of blue-1 and yellow-5 food dyes were taken from the labels in their stock containers. The equations C1*V1=C3*VVF and C2*V2=C4*Vvf were used to determine the volumes needed for the final solution and Vvf-V1-V2= the amount of deionized water needed. Two burets were cleaned with deionized water and then filled with each food dye to the maximum measured amount the burets could hold. Then, the burets transferred the amount of each dye needed to the final solution to recreate the concentration found in the green apple Gatorade. Deionized water was added with a clean 5 mL pipet and small pipet till the solution was leveled with the mark. The solution was then mixed well together and transferred into the clean 1 cm cuvette, which was placed into the spectrometer to determine if the solution matched the final concentration. The wavelength and absorbance of the two peaks were recorded and compared to the data recorded from the green apple Gatorade measured previously (λ3 A3 and λ4 A4 with λ1 A1 and λ2 A2). The percent error of the two absorbance values were calculated using the equations, %=A3-A1/A1 and %=A4-A2/A2. The percent’s calculate showed the accuracy of the
Chromatography is a method of separating and analyzing complicated substances. This is done in two phases, a mobile phase and a stationary phase .During the stationary phase, said substance is stationary, while during the mobile phase, the substance moves in a specific direction. During the mobile phase, the substance is filtered through the stationary phase. The stationary phase in necessary in order for the substances to be separated even though it doesn?t involve movement of the substance because it filters the substance through the stationary phase.. Since the substance is made of different, specific substances, each can go though the process of chromatography at different rates. This causes the components of the substance to be moved over materials made for absorption at different times. This makes the different components of the substance absorb at different rates. This is done numerous times and is a very precise method of separation. This process can be used to separate a wide variety of things, and can be used to separate most volatile or soluble substances. This process is used many like because it is gentle enough to separate delicate solutions, like those of proteins.
varying pH levels. After the paper is dipped into the liquid and the color changes, the robot will
As stated before, ink is made of components with varying polarity and in order to separate these components we must determine the liquid and stationary phase that does this best. One of the three suspected inks is extracted with a 50% ethanol mixture and dotted onto 7 pieces of paper to be dipped in varying solutions. Each solution varies in polarity with the stationary phase held constant (paper). The solution with the greatest separation of the components within the ink will then be used on a TLC plate (polar) to test all four inks (3 suspected inks a...