Bradford Assay Lab Report

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The concentration of the following solutions can be measured using a spectrophotometer. In our lab, we will be using the Bradford Assay procedure to measure the concentration of our protein samples. A spectrophotometer is a machine that passes light through a cuvette that can detect how much light a solution can absorb (Absorbance). The absorbance from our measured unknowns can be used to determine the concentration of protein in the solution. If any of our test solutions produce the highest absorptivity, then that solution has the highest concentration of protein because more protein more protein molecules will bind to the Coomassie dye, yielding a dark blue color. If the brand company of muscle milk claims their product have more protein, then we expect to have a high absorptivity and dark blue color for Muscle Milk than the other

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