Designing, Creating and Calibrating a Timing Device
Overview:
Using our knowledge from Biology, Chemistry and Physics, we were to
design a timing device to measure the length of music played on the
final day of this project. In our group, we separated into three
teams to create three different timing devices. This report is on one
of the timing devices, named the Fermentation Clock created by Elaine
Wong and Aki Kuboyama.
The Fermentation Clock is a simple device in which dilute hydrogen
peroxide is added to yeast, which is one of the Biology experiments
for studying fermentation. As the yeast reacts with hydrogen
peroxide, it releases heat energy at a fairly consistent rate. By
using the temperature-time relation in this experiment, we calibrated
a standard curve which was later used to measure the length of music.
Further more, before calibration, we experimented with different
concentrations of hydrogen peroxide as well as different masses of
yeast to find the best amount of each to create the most suitable
standard curve. We found that 180mL of water and 20mL of hydrogen
peroxide added to 1.00g of yeast gave the most consistent rate of
temperature change from 2 to 6 minutes which was the predicted length
of time of the music. Needless to say, the process of testing these
concentrations comes from the basic principles of Chemistry
experiments.
Also, to increase the efficiency of the device, we insulated the
beaker of yeast with cotton and styrene foam so the maximum heat will
be applied to the thermometer, an aspect of Physics. This is
important because the greater the temperature rise, the more accurate
the approximation. We considered placing the beaker of yeast into a
styrene foam container to maximise the temperature rise but
unfortunately, could not find any suitable containers so this was
eluded.
Materials:
±0.0001˚C±2%
180mL
20mL
1.00g
4000.0±0.1g±2%
100±1mL
100.0±0.5˚C
400mL
600mL
To have a control, we put 11mL of distilled water with the yeast in a fourth vial. After this, we place the top on each vial, created a seal and
The Effect of Temperature on the Action of Peroxidase Enzyme Aim To find the effect of temperature on an enzyme in this case peroxidase, by studying it decomposing hydrogen peroxide. Planning I am measuring the amount of gas given off in the reaction. By collecting this figure I can determine the rate of reaction. If I know the rate of reaction I can find out what temperature peroxidase works best at. Equipment · Trough · Clamp · 100ml measuring cylinder · Stop clock · 10ml measuring cylinder · Delivery tubes · Bung · Conical flask Variables Change
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Equipment and Materials 3 conical flaks 3 delivery tubes Spatula Balances Fridge Ammonium Sulphate (0.5) Yeast powder (1g)
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