What Is The Effect Of Sucrose Concentration On Tyrosinase Enzyme Activity

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By using spectrophotometer, the effect of enzyme and substrate concentrations were predicted. The experimental design was to observe the effect of particular substrate concentrations on tyrosinase enzyme activity. Any changes in amount of product formed over a time, depended upon the level of enzyme present. Likewise, an enzyme is very specific meaning that, it can recognize its substrate from even closely related isomers. The specificity of tyrosinase for the substrate tyrosine is not high. In fact, it reacts with a variety of ring structures and one of them is Catechol. Sucrose is not affected by the tyrosinase enzyme. So it was hypothesized that there will be no brown color as production when sucrose will be involved. On the other hand,

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