Methionine Lab Report

Methionine represents the first limiting amino acid in broiler nutrition, thus different sources are available to balance diets based of corn and soybean. Bioavailability is different for each methionine source because of its rate of absorption and metabolic pathways. A broiler experiment was conducted to determine the relative bioavailability of Hydroxyl Methyl Analog Calcium (HMA-Ca) relative to DL-Methionine(DL-Met). The experiment was conducted at at Lavinesp (Unesp, Jaboticabal). It was used 1890 male broiler Cobb 500 of 21 days old, they were weighted and distributed homogeneously in a complete randomized design with 13 treatments and 7 replicates each. All birds fed either a basal diet deficient in sulphur amino acids, digestible methionine and cysteine (dig Met+Cys), or the basal diet with four levels of HMA-Ca (0.063, 0.183, 0.302 and 0.540%) and DL-Met (0.054, 0.156, 0.259 and 0.463%) to achieve increasing levels of dig Met+Cys. For the analysis, 5% of significance was considered and procedures of non-linear model were used by SAS. Exponential regression determinates bioavailability of HMA-Ca relative to DL-Met by calculating the relation of the slope of HMA-Ca relative to DL-Met

Furthermore, equations obtained for carcass parameters were CY = 74.914 + 2.592 * (1-e – (8.218 * DL-Met + 3.976 * MHA-Ca)), AF = 2.246 + 0.573 * (1-e – (8.063 * DL-Met + 7.813* MHA-Ca)) and BY = 23.508 + 5.460 * (1-e – (5.546 * DL-Met + 3.131 * MHA-Ca)). Where CY is carcass yield, AF is abdominal fat and BY is breast yield. Thus, bioavailability of HMA-Ca relative to DL-Met were 52.43% for BWG and 57.48% for FC. Biological efficacies for CY, AF and BY were 42.57%, 87.02% and 49.69%, respectively. These results show that bioavailability of HMA-Ca relative to DL-Met are 54.955% for performance and 59.76% for carcass parameters on a product