Catalase Enzyme Lab Report

Aim

This experiment attempts to investigate how the temperature change affects the Activity of Catalase Enzyme (rate of reaction).

Research Question

What is the effect of temperature on the activity of catalase enzyme?

Background Information

Enzymes are protein which acts as catalysts throughout a biochemical reaction to help increase enzyme activity (rate of reaction). That different types of enzymes played vital role in the human body in carrying out corresponding functions, such as digestion. Such that catalase is one of the most widespread enzymes. Enzyme activity is therefore affected by surrounding environmental factors. A change in these environmental factors alter the enzyme activity thus the rate of reaction. Moreover, different enzymes produce different optimum rate in which the maximum

As temperature increase, there will be an increase in the kinetic energy in molecules. Thus that should cause more of collisions between molecules per time in liquid form. Therefore the increase in random collisions with substrate molecules respectably increases the rate of reaction. However, there remain risk of bond breaking caused by increase in Vibrational Energy leading to decrease in the rate of reaction.

Variables

Independent Variable (IV)

Air Temperature (0°C; 20°C; 37°C; 45°C)

The independent variable is the air temperature where the experiment would be held in different temperature ranging accordingly from 0°C, 20°C, 37°C, and to 50°C. The experiments would be carries out at different location with the corresponding temperature listed above.

Dependent Variable (DV)

Rate of Reaction

The dependent variable is the rate of reaction.

Controlled Variables (CV)