Azithromycin is an antibiotic that targets gram-positive bacteria, and it inhibits growth by binding to the 50s ribosomal RNA to prevent protein synthesis. Through the Kirby-Bauer test, it was found that the gram-negative enteric bacteria were susceptible to Azithromycin. Azithromycin is a hydrophobic antibiotic, which means that the outer membrane of the enteric bacteria is capable of resisting the detrimental affects of Azithromycin (Vaara, 1993). The susceptibility of our isolate A bacteria may be due to a mutation that affects the outer membrane of the gram-negative bacteria; consequently, the antibiotic is able to penetrate the bacteria and bind to the ribosome. Mutant strains of the enteric bacteria Escherichia coli and Salmonella typhimurium that are unable to synthesize lipid A and lipopolysaccharide, components of the outer membrane, were found to be more susceptible to Azithromycin since the antibiotic was able to diffuse through the outer membrane (Vaara, 1993).
Penicillin G targets gram-positive and gram-negative, cocci bacteria and is expected to kill Isolate A by...
Azithromycin is Azithromycin is a macrolide antibiotic active in vitro to treat major pathogens that cause infections of the respiratory tract, other tissues and according to Wilson, Hannon, and Shields, they are: “bacterial infections, acute bacterial sinusitis, otitis media, pneumonia, tonsillitis, or pharyngitis.”(2014) It is manufactured under the trade name such as Azasite, Zithromax, and Zmax. Azithromycin acts by getting in to the 50S ribosomal subunit of susceptible microorganisms and blocked their regular protein making process.
Rheumatoid arthritis is a chronic inflammatory and an autoimmune disease that occurs when the immune system mistakenly attacks the body’s tissue (Rheumatoid arthritis, 2017). This disease affects the entire body, which is called a systemic (means entire body) disease. Arthritis is derived from the word part arthr-, which means “joint,” and -itis, which means “inflammation,” so altogether it means “inflammation of the joints.” It creates inflammation that causes the tissue that lines the inside of joints (synovium) to thicken. About 1.5 million people in the U.S. are affected. It affects all races, but it affects three times as many women than men (What is Rheumatoid Arthritis, n.d.). Overtime, rheumatoid arthritis causes painful swelling that can potentially result in bone erosion or joint deformity, which leads up to physical disabilities. RA can affect more than just your joints, but can spread to body systems, skin, eyes, lungs, heart, blood vessels, e.t.c (Rheumatoid arthritis, 2017).
Tobramycin is effective at reducing growth and reproduction of gram-negative bacteria. The bacteria P. aeruginosa, Klebsiella pneumoniae, Escherichia coli, Proteus, Serratia, Acinetobacter, Staphylococcus aureus are susceptible to Tobramycin. When treating enterococcal infections, which are part of the normal intestinal flora of humans, the addition of penicillin is needed. Tobramycin is used to treat external ocular infections, Urinary tract infection, Pseudomonas infection, Staphylococcus bacteria infection, and Respiratory Tract Infections. To reduce the creation of antibiotic-resistant bacteria, and to maintain the efficiency of Tobramycin, this ...
On September 18th and the weeks following, a multitude of letters containing the Bacillus Anthracis bacterium were mailed to various New York news stations. Individuals at 5 different stations became ill with similar symptoms that included vomiting and shortness of breath. This began the nationwide panic known as the Amerithrax Investigation. The Amerithrax investigation was said to be the worst biological attacks in all of US History. Three weeks later, on October 9th, two more letters, containing the anthrax bacterium was mailed to two Democratic senators. In all of the 7 attacks, over 22 people developed infections, over half being life-threatening. The letters themselves confused federal investigators. In the sets of letters addressed to the media, certain T’s and A’s were bolded. When added together, the groups of letters formed 3 codons that corresponded to 3 amino acids. These amino acids pointed federal investigators towards a possible culprit, Bruce Edwards Ivins, a scientist for the US government in Frederick, Maryland.
Acquired antimicrobial resistance generally can be ascribed to one of five mechanisms. These are production of drug-inactivating enzymes, modification of an existing target, acquisition of a target by-pass system, reduced cell permeability and drug removal from the cell. (Sefton) Also a bacterium that was once prone to an antibiotic can gain resistance through alt...
Acid is produced naturally in your stomach to help you digest food and to kill bacteria. This acid irritates the stomach lining so our body produces a natural mucus barrier which protects it. Sometimes this barrier may be damaged thus allowing the acid to damage the stomach causing inflammation, ulcers and other conditions. Other times, there may be a problem with the muscular band at the top of the stomach that keeps the stomach tightly closed and this allows the acid to escape and irritate the oesophagus. This is called 'acid reflux' and can cause heartburn and/or oesophagitis. Proton pump inhibitors such as omeprazole stop cells in the lining of the stomach from producing too much acid. This can help prevent ulcers from forming or assist the healing process. By decreasing the amount of acid, they can also help to reduce acid reflux related symptoms such as heartburn.
“But how did it come to this?” you’re probably asking yourself. Humans may have been studying antibiotics, but so were bacteria – and they’ve b...
Streptococcus pneumoniae is a Gram-positive and fast-growing bacteria which inhabit upper respiratory tract in humans. Moreover, it is an aerotolerant anaerobe and usually causes respiratory diseases including pneumonia, otitis media, meningitis, peritonitis, paranasal sinusitis, septic arthritis, and osteomyelitis (Todar, 2003). According to Tettelin et al., more than 3 million of children die from meningitis or pneumonia worldwide (2001). S.pneumoniae has an enzyme known as autolysin that is responsible for disintegration and disruption of epithelial cells. Furthermore, S.pneumoniae has many essential virulence factors like capsule which is made up of polysaccharides that avoids complement C3b opsonization of cells by phagocytes. Many vaccines contain different capsular antigens which were isolated from various strains (Todar, 2003). There are plenty of S.pneumoniae strains that developed resistance to most popular antibiotics like macrolides, fluoroquinolones, and penicillin since 1990 (Tettelin et al., 2001). Antibiotic resistance was developed by the gene mutation and selection processes that, as a consequence, lead to the formation of penicillin-binding proteins, etc. (Todar, 2003).
Approximately one year ago in Kentucky, a man went to sleep thinking he might have caught a flu. The next day, he is rushed to the local hospital while coughing up chunks of lung tissue; within a few hours he experiences organ failure and lips into a coma. Over the next two days, two other patients come in with the same symptoms and die almost immediately. This epidemic that swept over this small area in Kentucky was an ultra resistant strain of staph infection known as MRSA, or methicillin-resistant Staphylococcus aureus (Eisler, 2013). MRSA and other species of resistant bacteria have arisen from the global overuse of antibiotics. Over the years, resistant strains of bacteria have become more and more difficult to fend of using common antibiotic treatments. If something is not done to stop antibiotic resistance, completely resistant strains of bacteria, which we will be unable to kill through use of antibiotics.
Bacterial resistance to antibiotics has presented many problems in our society, including an increased chance of fatality due to infections that could have otherwise been treated with success. Antibiotics are used to treat bacterial infections, but overexposure to these drugs give the bacteria more opportunities to mutate, forming resistant strains. Through natural selection, those few mutated bacteria are able to survive treatments of antibiotics and then pass on their genes to other bacterial cells through lateral gene transfer (Zhaxybayeva, 2011). Once resistance builds in one patient, it is possible for the strain to be transmitted to others through improper hygiene and failure to isolate patients in hospitals.
Pre-clinical testing is performed to Good laboratory practice (GLP) and covers pivotal toxicology & safety pharmacology studies. In preclinical research, scientists test their ideas for new biomedical prevention strategies in laboratory experiments or in animals.
...d rectal thermometers and transmission by hands after touching IV or urinary catheters. Enterococci can be intrinsic and can tolerate or resist beta-lactam antibiotics due to containing penicillin-binding proteins. That means they are still able to combine cell wall components. There can be acquired resistance of Enterococci that comprises of resistance to penicillin by beta-lactamases, chloramphenicol, tetracyclines, rifampin, fluoroquinolones, aminoglycosides, and vancomycin. There is a potential for cell-wall synthesis because the genes that encrypt intrinsic or acquired vancomycin resistance produce in a peptide to which vancomycin cannot connect. Unfortunately, due to the resistance of penicillin, Enterococci can be inhibited but cannot be killed. Health care professionals are left with limited therapeutic therapy that can be effective in the treatment of VRE.
Resistance first appears in a population of bacteria through conditions that favor its selection. When an antibiotic attacks a group of bacteria, cells that are highly susceptible to the medicine will die. On the other hand, cells that have some resistance from the start or acquire it later may survive. At the same time, when antibiotics attack disease-causing bacteria, they also attack benign bacteria. This process eliminates drug-susceptible bacteria and favors bacteria that are resistant. Two things happen, populations of non-resistant and harmless bacteria are diminished, and because of the reduction of competition from these harmless and/or susceptible bacteria, resistant forms of disease-causing bacteria proliferate. As the resistant forms of the bacteria proliferate, there is more opportunity for genetic or chromosomal mutation (spontaneous DNA mutation (1)) or transformation, that comes about either through a form of microbial sex (1) or through the transference of plasmids, small circles of DNA (1), which allow bacteria to interchange genes with ease. Sometimes genes can also be t...
Bacteria are found nearly everywhere within the body and most types are harmless or even helpful to bodily function (Novitt-Moreno). While it is important to have these bacteria in the body, pathogenic invaders can cause serious illnesses. Pathogenic bacteria work by either actually attacking a part of the victim’s body or releasing toxic waste products into the body. Bacteria are single-celled and contain all of the cellular mechanisms needed to live, grow, and reproduce (Novitt-Moreno). That means, when treating a bacterial infection, it is critical to have a highly specific antibiotic that can destroy the unwanted
Infection control, a term that describes procedures taken to reduce the spread of infection. The dental office is a place where many people are treated including patients with infectious disease such as tuberculosis, HIV/AIDS, hepatitis, and many other highly contagious diseases. It is imperative that in any dental office setting the prevention of the spreading microorganisms from patient to patient, patient to staff, or staff to patient is done in high precaution. Infection control has two main objectives; to protect the patients from harmful pathogens as well as dental team members. Infections can cause or add pain, deteriorate a persons health, and in worst cases even result in death. In order to understand the infection control in a dental facility, you must understand the standard precautions required by organizations that regulate or recommend infection control, the kinds of preventive measures taken, as well as when these measures should be taken.