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...]. Although these advances were unquestionably important, they lacked an easy way to modify light output. Therefore, we propose a re-usable biological light switch that can toggle between ON and OFF states within a reasonable amount of time. To do this, we used the concept of a bi-stable switch in which each module is induced by a specific chemical inducer molecule. In our system, L-Arabinose (ON) and Allolactose (OFF) were chosen as the inducers because the E. coli strain used (BL21(DE3)) contains endogenous metabolic pathways that can degrade them. This allows us to revert our switch back to the initial un-induced state, thus allowing the user to reuse this switch through multiple cycles [14, 16 17]. In conclusion, this innovation will allow bioluminescence to be used in a number of diverse applications while promoting awareness for unconventional lighting models.
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