We Propose A Reusable Biological Switch Essay

We Propose A Reusable Biological Switch Essay

Length: 703 words (2 double-spaced pages)

Rating: Better Essays

Open Document

Essay Preview

We propose a reusable biological switch that is capable of toggling between on and off states within a reasonable amount of time. To accomplish this, we will use biologically modified Escherichia coli transformed with a bi-stable switch-containing plasmid. A bi-stable switch is a genetic component that allows for the continuous expression of one of two phenotypic states. Toggling between these two states is controlled by the presence of specific chemical inducers [16]. The two states in our proposed design will be light production (ON) and the absence of light production (OFF). For recyclability, the choice of inducers is crucial. Our inducers need to be naturally metabolized by E.coli to allow for our system to reset after each induction event. Our circuit contains the Lux operon and the Lac repressor (LacI) under the control of the L-Arabinose-inducible pBAD promoter and the pBAD repressor (araC) under the control of the Allolactose-inducible Lac promoter. This DNA assembly is inserted into pSB1A30, a high copy number plasmid backbone containing a multiple cloning site and an Ampicillin resistance gene [1, 11, 12]. For our inducers, L-Arabinose (ON) and Allolactose (OFF) are chosen because the E. coli strain used (BL21(DE3)) contains endogenous metabolic pathways for the degradation of these two compounds [14, 17]. This innovative technology may be useful in numerous applications including, but not limited to: diffuse lighting for large areas, decorative fixtures, and electronically pre-timed lighting. We hope that our proposed prototype will generate awareness for unconventional lighting methods, specifically bioluminescence, while providing a means of reducing the energy consumption, greenhouse gas emission, and material/tox...

... middle of paper ...

...]. Although these advances were unquestionably important, they lacked an easy way to modify light output. Therefore, we propose a re-usable biological light switch that can toggle between ON and OFF states within a reasonable amount of time. To do this, we used the concept of a bi-stable switch in which each module is induced by a specific chemical inducer molecule. In our system, L-Arabinose (ON) and Allolactose (OFF) were chosen as the inducers because the E. coli strain used (BL21(DE3)) contains endogenous metabolic pathways that can degrade them. This allows us to revert our switch back to the initial un-induced state, thus allowing the user to reuse this switch through multiple cycles [14, 16 17]. In conclusion, this innovation will allow bioluminescence to be used in a number of diverse applications while promoting awareness for unconventional lighting models.

Need Writing Help?

Get feedback on grammar, clarity, concision and logic instantly.

Check your paper »

The Biological Factors, And The Free Will Essay

- When looking at a person’s life, there are many factors to consider, social strains, biology, free will, and up-bringing to name a few. Each of them is very important, but today I will e discussing the biological factors, and the free will. Free will is the ultimate or final decider of one’s life path, and although factors of determinism might give one their foundation or starting point, free will takes you the rest of the way. That being said, it is important to remember that free will and determinism are not mutually exclusive....   [tags: Free will, Determinism, Biological determinism]

Better Essays
1062 words (3 pages)

Biological Weapons Convention Treaty Essay

- The Biological Weapons Convention treaty also known as the Biological and Weapons Toxin Convention was established in 1972. This treaty was created in the efforts to stop the production of Biological weapons and the use of it in combat. As well as banning the use of its delivery systems and storage facilities. In 1972 over 158 countries came to an agreement and signed the Biological Weapons Convention treaty. Although the treaty was signed in 1972 it did not take effect until 1975. This treaty only affected the countries who actually signed it....   [tags: biological warfare, toxins]

Better Essays
893 words (2.6 pages)

A Biological Warfare Attack On Agriculture Essay

- A biological warfare attack on agriculture is based on the motive of all terrorist attacks; to elicit fear and anxiety among the public (Roberge n.p.). With this in mind, terrorists have many avenues of attack in regards to our agriculture and food industries. Each section, or characteristic, of this industry has its own areas of security over watch as well as unique security weaknesses. How then could a terrorist attack our agriculture and food industries and how do these characteristics increase our risk....   [tags: Food industry, Food, Biological warfare]

Better Essays
720 words (2.1 pages)

The Biological Integrated Detection System Essay

- The Biological Integrated Detection System The Biological Integrated Detection System is a United States Army asset that provides the ability to alert, detect, analyze, identify and report the presence of biological agents on the battlefield. In today’s world of ever expanding and uncertain chemical, biological, radiological, and nuclear (CBRN) threats, it is more important than ever to understand the availability of tools used to protect the populace. In the United States of America, the citizenry is fortunate to have the United States Army as a front line resource that maintains elements within its organization to guard against these threats....   [tags: US army, battlefield, biological threats]

Better Essays
1072 words (3.1 pages)

Essay on Getting Mr. Right to Propose

- Men and women think alike on a lot of things, but marriage seems not to be one of them. Women think about marriage when they feel like they have met the right man; however, men think about marriage when they feel like they are ready and of the age to get married. These differences cause misunderstandings between the two sexes. If a woman thinks she has met the right man to be her husband, she has to keep him interested, be a good girlfriend to him, and express her interest in marriage to him. There are no differences between men and women when it comes to being happy in a relationship....   [tags: marriage, interest, girlfriend]

Better Essays
550 words (1.6 pages)

Essay on Biological Integrated Detection Systems

- There are many countries that are believed to have established programs in offense to biological warfare. Some of the distinguished countries include: Syria, Russia, China, Iran, Israel, Egypt, and North Korea. The easiest way to begin explaining Biological warfare is to consider any living organism and its possible potential to be used as a way of causing harm, injury, and even death to any population of people (Hooker, 2014). Organisms may consist of bacteria, fungi, or viruses. There are also toxins that may be found in any natural environment that have the potential to be used as a biological attack on any one person or population....   [tags: biological warfare, Syria, Russia, Egypt]

Better Essays
913 words (2.6 pages)

Reusable Software Development Essay

- The quality and reliability of software today is expected to be extremely high. Software is being included in engines of automobiles, robots, x-ray machines at hospitals; it is no surprise that software is expected to operate correctly almost all the time. This reliability comes at a cost of increasing complexity. With the increase in complexity, software development teams are getting larger and maintenance is becoming more difficult. Although developers are dealing with these problems project dates are still very rigorous and projects can seem impossible to complete on time....   [tags: Software Engineering]

Better Essays
2313 words (6.6 pages)

The Dangers of Military Biological Testing Essay

-     Abstract: Across the United States for the past half a century, the Department of Defense and the U.S. Army have been releasing biological agents over the very population they are supposed to protect. Although few Americans know it, open-air biological testing of stimulants such as aspergillus fumigatus, zinc cadmium sulfide, bacillus subtilis, and serratia marcescens has been taking place over populated areas since at least 1949. Between 1949 and 1969, the military admits to having conducted 239 open-air tests over populated areas in the United States....   [tags: Biological Testing Essays]

Better Essays
1731 words (4.9 pages)

Biological Basis Of Behavior Essay

- Biological Basis Of Behavior Primary Behaviors of Schizophrenia Schizophrenia is defined as a "devastating psychotic disorder that may involve characteristic disturbances in thinking (delusions), perception (hallucinations), speech, emotions, and behavior (Durand and Barlow 443). This disorder affects nearly 2.5 million people. The symptoms of schizophrenia are usually divided into two categories, positive and negative. The positive symptoms include delusions, hallucinations, disorganized speech or thinking, grossly disorganized behavior, and catatonic behaviors, which means that you suffer from motor immobility (schizophrenia.com)....   [tags: Science Biology Biological Essays]

Better Essays
1583 words (4.5 pages)

Essay on The Threats of Biological Warfare

- The term “biological warfare” has been used quite frequently lately. We see it on the news, read it in magazines and newspapers, and hear about it in the political rhetoric of the day. However, the sad reality is that most Americans are not well informed about how dangerous the threat of biological warfare really is. Not only that, but our own government is not even prepared to deal with a biological attack, something that is more probable than most of us would like to believe. Biological warfare is not all that complicated....   [tags: Biological Warfare War Weapons Essays]

Better Essays
2318 words (6.6 pages)