Investigating the Effect of the Enzyme Catalyse On Hydrogen Peroxide

Investigating the Effect of the Enzyme Catalyse On Hydrogen Peroxide

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Investigating the Effect of the Enzyme Catalyse On Hydrogen Peroxide

Introduction

The aim of this experiment is to determine the effects of varying
enzyme (catalyse) on Hydrogen Peroxide.

Hydrogen Peroxide + Catalyse à Water + Oxygen

2H2O2 à H2O + O2 + Heat

Apparatus & Diagram

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Bung

Potato

Hydrogen Peroxide

Water

Collected Oxygen

Delivery Tube

Measuring Cylinder

[IMAGE]

Using the Equipment Safely

It is important that we use the apparatus carefully, as safety will be
an issue throughout the whole experiment. We will wear goggles and an
apron or lab coat to protect our eyes and clothes. As we are using
enzymes and Hydrogen Peroxide we need to be extra careful, ensuring
they don't come into contact with our eyes, skin or clothes.

Catalyse is an enzyme found in all living cells. It makes Hydrogen
Peroxide decompose into water and Oxygen. We will be measuring the
amount of Oxygen released from the Hydrogen Peroxide. In order to do
this we will use a measuring cylinder. This piece of apparatus
measures the amount of Oxygen given off, measuring in cm3. This will
help us measure the amount of Oxygen more accurately.

To make the test fair, the following parameters must remain constant
during the experiment. These parameters must remain constant during
the experiment. These parameters are water, Hydrogen Peroxide,
Catalyse and the duration of the reactions. By insuring the test is
fair, we will gain accurate results.

Variables

Dependant Variable: Time, Size of 5cm piece of Potato

Independent Variable: Amount of Oxygen released

Control Variable: Volume of Hydrogen peroxide, size of Potato,
concentration of Hydrogen Peroxide

Hypothesis

I predict that the breakdown of Hydrogen Peroxide will be quicker when
the surface area is increased. If you cut the same size piece of
potato into smaller pieces, I believe, the breakdown will be faster. I
predict that an increase in surface area will result in an increase in

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Related Searches

kinetic energy. Since the size of the area increases, I think that
more oxygen will be released from the Hydrogen Peroxide and Catalyse.

We will be measuring the two main factors: -

* Oxygen - the amount released from the Hydrogen peroxide

* Time - The duration of the reaction

The room temperature may be a factor that will affect our results.
Therefore we will have to try our bests to inure the test is carries
out under the same conditions.

To make it fair we will allow the reactions to have the same amount of
time to occur. We will only change the amount of catalyse in the tube.

To obtain the best possible results I will repeat each experiment
three times and then find the average set of data to plot my graph.
Our measurements will be very precise as we will carry out the
experiment as safely and fairly as possible. We are using accurate
apparatus to allow us to achieve this.

Investigation Experiment

I am going to investigate how the enzyme, Catalyse reacts at different
surface area sizes. The procedure for the experiment is as follows;

v Set up the apparatus

v Place the water in the water bath

v Fix the inverted measuring cylinder so that the delivery tube
connects to the boiling tube

v Using a measuring cylinder, measure 10ml of Hydrogen Peroxide

v Measure 5sm of potato and add to the Hydrogen Peroxide solution in a
boiling tube

v Immediately place the bung on the boiling tube, as the reaction
would have already started

v Set the timer for 3 minutes. Repeat the experiment three times (for
accuracy) using the different pieces of potato

v It is important only the size of the potato is changed, since that
is what is being investigated

Results

Time (Secs)

Attempts

One Piece

Two Pieces

Three Pieces

Four Pieces

Five Pieces

30

1

1

1

2

2

3

2

1

1

2

2

1

3

1

1

2

1

4

Average

1

1

2

1.67

2.67

60

1

3

2

5

7

5

2

3

2

5

5

9

3

5

3

5

5

7

Average

3.67

2.3

5

5.67

7

90

1

8

7

7

9

6

2

8

6

8

9

12

3

9

6

9

10

14

Average

8.3

6.3

8

9.3

10.67

120

1

10

10

9

12

9

2

10

11

8

11

11

3

11

11

8

11

15

Average

10.3

10.67

8.3

11.3

12.3

180

1

12

11

12

17

12

2

12

14

13

17

16

3

13

15

14

15

16

Average

12.3

13.3

13

16.3

14.67

210

1

13

16

15

20

15

2

13

16

16

20

20

3

14

16

15

21

20

Average

13.3

16

15.3

20.3

18.3

Scientific Knowledge

They way enzymes work is that they have a lock and key mechanism. The
catalyse can only be broken down when the enzyme has the correct
combination that will fit into the catalyse and allow it to be broken
down.

Conclusion

My results proved that my hypothesis to be correct. The breakdown of
Hydrogen Peroxide accelerated as the surface area increases.

With less surface area the rate of reaction is not as much as it could
be. When you cut the potato into smaller pieces this allows a greater
surface area to be used by the Hydrogen Peroxide. Therefore the rate
of reaction increases.

Enzymes are specific in the reactions they catalyse, much more so than
inorganic catalysts. Normally, a given enzyme will catalyse only one
reaction, or type of reaction. The enzyme has an active site that
helps it to recognise it's substrate in its own specific lock; each
enzyme has its own specific substrate. This is called the lock and key
theory. The enzymes never actually get consumed in the process; they
just increase the rate of reactions.

Evaluation

Overall I would like to think my experiment went alright. Almost
everything went according to plan. We followed our safety instructions
and did the experiment fairly and accurately as possible. Therefore
the majority of my results came out to be reliable and accurate. Due
to using the equipment safely and accurately this experiment has been
successful.

However, there were a few results in the experiment that do no agree
to the scientific information. When measuring the amount of Hydrogen
Peroxide, we may have not measured the exact amount. We could have
accidentally measured 10.1ml; instead of 10ml. another problem was the
temperature of the room. As we carried out the experiment on different
days, some times the temperature in the room varied, from 23C to 30C.
This may have affected the particles in the enzymes, causing the
reaction to occur slightly faster than it should or slightly slower.
Luckily there were few spillages of Hydrogen peroxide, as we used the
equipment safely and accurately. There was air/water in the delivery
tube prior to the reaction which may have delayed the oxygen getting
through. Also the condition and variety of potato could have affected
the results because some the potato could have been four weeks old and
the others only just freshly cut.
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