Investigating the Best Temperatures betweem Calf Trypsin and Fugal Trypsin

Investigating the Best Temperatures betweem Calf Trypsin and Fugal Trypsin

Length: 1011 words (2.9 double-spaced pages)

Rating: Excellent

Open Document

Essay Preview

More ↓
Investigating the Best Temperatures betweem Calf Trypsin and Fugal Trypsin



Trypsin is an enzyme so to be able to conduct a suitable plan, this
idea will help me to predict the best temperature that can be achieved
on its activities in different conditions. “Collins Advanced Science
Biology defines an enzyme as biological catalysts, speeding up
reactions that would otherwise happen too slowly to be any use to the
organism, that is it has catalytic properties, in other words they
alter the rate of reaction without themselves undergoing a permanent
change. Most chemical reactions require an initial input of energy
called activation energy, to enable them to occur. Enzymes reduce the
need for activation energy and so allow reactions to take place more
readily than would otherwise be. An active enzyme may speed up a
particular reaction, but living organisms do not need all reactions to
be going at the maximum rate all of the time. It would be perfect to
say after considering this fact that, enzymes do actually control
rather than speeding up, because they interact with other molecules to
produce an ordered, stable reaction system in which the products of
any reaction are made when they are needed in the amount needed.
Enzymes are globular proteins. They have complex tertiary structure in
which polypeptides are folded around each other to form a roughly
globular shape. This ship is very important once altered, the enzyme
cannot bind to its substrate and so cannot function, this shape is
maintained by hydrogen bonds and ionic forces and their function can
be affected by changes in temperature and pH, these are not the only
factors that can affect enzymes are competitive inhibitors they
compete with the substrate for the active site, The greater the
concentration of the substrate the more likely it is to occupy the
active sites and the less the effect of the inhibitor. and
non-competitive inhibitors which attach themselves to the enzyme other
than the active site, As the substrate and inhibitor are not competing

How to Cite this Page

MLA Citation:
"Investigating the Best Temperatures betweem Calf Trypsin and Fugal Trypsin." 27 Feb 2020

Need Writing Help?

Get feedback on grammar, clarity, concision and logic instantly.

Check your paper »

Essay on Short Story: The Puppy and the Calf

- The Puppy and the Calf Kivah sunk back into her big-kids seat, petting her baby poodle Buttercup for comfort. She closed her eyes tightly, as if though she was invisible to her father who was driving. Opening her eyes she looked up into the rare-view mirror, where her eyes and her dad’s eyes met. “What’s the matter honey?” Her father asked. With teary eyes she replied, “I don’t want to stay with grandma and grandpa on the ranch. I want to be home with you and mommy, PLEASE!” “Oh baby. I promise you, you will have fun on the ranch....   [tags: eyes, country, calf, body]

Research Papers
836 words (2.4 pages)

Investigating How Quickly Amylase Breaks Down Starch With Varying Temperatures

- Investigating How Quickly Amylase Breaks Down Starch With Varying Temperatures Instructions List I gathered the equipment shown below. I put 3 drops of iodine in each chamber of the spotting tile. I boiled the kettle and mixed together water from the kettle with water from the tap so the water reaches the correct temperature. The water was put into a beaker. 5ml of starch was put into a test tube. The test tube was placed into the beaker. When the water in the beaker was at the required temperature the stop clock was started....   [tags: Papers]

Free Essays
607 words (1.7 pages)

Investigating the effect of Temperature on the reaction between Sodium Thiosuphate and hydrochloric acid

- Investigating the effect of Temperature on the reaction between Sodium Thiosuphate and hydrochloric acid · Planning I am investigating the different temperatures on the reaction between Sodium Thiosuphate and Hydrochloric acid. The factors I could investigate would be temperature, concentration and substances. I have chosen to investigate Temperature. I predict that the higher the temperature the more rapidly the cross would obscure. This is because the higher the temperature the more often the particles collide and react, when the particles collide more often they would collide harder because they have more energy, making the collisions bigger and faster, the collision success rate is hi...   [tags: GCSE Chemistry Coursework Investigation]

Free Essays
1051 words (3 pages)

Investigating the Relationship Between Temperature and Resistance in a Themistor

- Investigating the Relationship Between Temperature and Resistance in a Themistor Aim To investigate the relationship between temperature and resistance in a thermistor. Introduction A current is the flow of charge round a circuit, this can be in the form of ions in a liquid or electrons in a metal. Resistance is anything that slows the flow of electrons round the circuit. Ohm's law states that the voltage is equal to the current multiplied by the resistance - V=IR This can be re-arranged to say R=V/I....   [tags: Papers]

Free Essays
1748 words (5 pages)

Investigating the Solubility of Table Salt in Distilled Water at Different Temperatures

- Investigating the Solubility of Table Salt in Distilled Water at Different Temperatures The aim of this essay is to explain and discuss the drug cannabis and its effects in relation to biochemical, behavioural and psychological factors. The introduction will outline a brief history relating to the drug and include both the medicinal and recreational purposes of its use. The main discussion will explain how the drug chemistry affects the nervous system emphasising what neurotransmitter is effected and also what behavioural and psychological implications this has on the user....   [tags: Lab Report]

Research Papers
2162 words (6.2 pages)

Investigating the Effect of Temperature on the Volume of a Gas Essay

- Investigating the Effect of Temperature on the Volume of a Gas Aim: To investigate the effect of temperature on the volume of a gas, given that the pressure acting on the gas is fixed. Hypothesis: According to CharlesÂ’s Law, the volume of a given amount of gas is directly proportional to the absolute temperature when the pressure is constant. Thus, it is expected that as the temperature of the gas increases, its volume will increase also at a constant rate. When the temperature is increased, the particles in the gas gain more kinetic energy and as a result, the particles move faster and quicker and thus, the particles bombard each other more frequently....   [tags: Papers]

Free Essays
656 words (1.9 pages)

Essay on Investigating the Effect of Temperature on the Rate of a Reaction

- Investigating the Effect of Temperature on the Rate of a Reaction Introduction: Magnesium + Hydrochloric acidàmagnesium chloride + hydrogen Mg+2HCl àMgCl2 + H2 This is the reaction I will use for my investigation into the effect of temperature on the rate of a reaction. I will increase the temperature and measure the volume of gas given off over a period of time. I have chosen to use temperature instead of concentration because I believe it will create better results and make an easier experiment....   [tags: Papers]

Free Essays
648 words (1.9 pages)

Investigating The Effect of Temperature on the Structure of an Enzyme Essay

- Investigating The Effect of Temperature on the Structure of an Enzyme Introduction: For my GCSE Biology assessment I will be investigating the enzyme amylase with the substrate starch. This reaction, which I am going to investigate, is called the protein test for starch. Aim: My intention for this observation is to examine how the enzyme catalyses are affected by changes in temperature. Safety Precautions: In this investigation I am going to make sure that everything is as safe as possible and prevent any accidents from occurring....   [tags: Papers]

Free Essays
1316 words (3.8 pages)

Essay about Investigatin Attraction Between People

- Investigatin Attraction Between People a) Similarity. For example similar backgrounds, ages, attitudes, beliefs and interests. The more they have in common the more likely a couple are to stay together. b) Familiarity. The more a couple meet up the more attractive they become to each another. In a 1968 study, Zajonc showed his participants a large number of photographs. He found that there was a positive correlation between the number of times a photograph was shown and the attraction....   [tags: Papers]

Free Essays
1950 words (5.6 pages)

The White Buffalo Calf Woman Essay

- The White Buffalo Calf Woman The Lakota Sioux Indians of the Great Plains possess rich religious traditions which are tied closely to the Earth. Though the relegation of these people to reservations amid the environmental disasters of American development has resulted in the near destruction of an ancient culture, some Lakota Sioux continue to fight for the preservation of their sacred lands animals, civil rights, and way of life. The seven original bands of the Great Sioux Nation were joined in an alliance called the “Seven Council Fires.” This confederation included three separate groups, each with its own dialect; the Santee spoke Dakota, the Yankton spoke Nakota, and the Teton spoke...   [tags: Papers]

Research Papers
863 words (2.5 pages)

Related Searches

for the same site, an increase in substrate concentration does not
diminish the effect of the inhibitor


As temperature increases, the molecules move faster, due to increased
energy. Therefore, the enzyme and substrate molecules will meet more
often and the rate at which the product is formed will increase this
is according to the Kinetic Theory. However, as the temperature
continues to raise the hydrogen and ionic bonds, which hold the enzyme
in shape, break and the active site will no longer accommodate the
substrate. The enzyme will denature and when this occurs it cannot be


Changes in pH may not only affect the shape of an enzyme but it may
also change the shape or charge properties of the substrate so that
either the substrate connot bind to the active site or it cannot
undergo catalysis. In general enzymes have a pH optimum. However the
optimum is not the same for each enzyme.


This is a simply hypothesis explaining the way that an enzyme breaks
up its substrate. It is recently known that the active site in many
enzymes is not exactly the same shape as the substrate, but moulds
itself around the substrate as the enzyme-substrate complex is formed.
The hypothesis explains my comment that enzyme binds to a substrate
momentarily, allowing a reaction to happen, but do not themselves
undergo any chemical change.

The image below explains the induced fit hypothesis better.



Trypsin is an enzyme secreted by the pancreas, it digest proteins
breaking them down into shorter chain of amino acids. I am going to
find the optimum temperatures between normal trypsin which is found in
the pancreas of most warm blooded animals and a genetically engineered
trypsin. I will do so by mixing and heating these different types of
trypsin and it’s substrate a suspension of powdered milk in various
water baths at various temperatures. This will help me determine the
various optimum temperatures of these different trypsins. I will
repeat the procedure several times at the different water bath and
then conclude my experiment.

I also know that enzymes work best at an optimum temperature. For warm
blooded enzymes such as trypsin it is close to the normal body
temperature (37°C). Below this temperature, the molecule of enzyme and
substrate have less kinetic energy and are moving less slowly and the
chances of colliding is high. At high temperatures (50°C) enzymes are
destroyed or denatured by heat. I will therefore carry out the my
experiment at range of temperatures from room temperature (20°C) to a
temperature that should cause denaturing(80°C)


I am going to use same concentration of trypsin in all my experiment,
If I increase the concentration it will mean that the chance of the
trypsin colliding with the substrate would be higher which would
affect the rate of reaction and my results as well, I would also use
the same concentration of my substrate throughout the experiment for
the same reason. The volume of trypsin and the milk suspension should
also be constant. If either varies this would upset the final
concentration of the enzyme and substrate which would make it
difficult to determine the optimum temperatures

The end point of the optimum temperatures is difficult to judge with
accuracy, so I will attempt to measure the volume of trypsin solution
using a graduated pipette, which I believe is accurate to 0.1cm3 which
is a 2% margin of error. I will repeat the experiment 3 times at each
temperature to check whether my findings are reliable


* Test tube rack

* Test tubes.

* Milk solution

* Trypsin 1(cow) and trypsin 2(fungal)

* Water bath

* Pipette

* Thermometer


Some people are allergic to enzymes so I will mop up any spillages and
will wash my hands after. I will take care when using the water bath.
I will also ware safety goggles and lab coat throughout.


1. Collect all equipment, and set out the water bath at temperatures
ranging from 20°C, 40°C, 60°C, and 80°C.Test with the thermometer to
check if the temperatures are set to the required ranges.

2. Measure 5ml of milk solution with graduated pipette into 8
different test tubes. Add 4 drops of each type of trypsin ( 4 drops of
calf trypsin and 4 drops of fungal trypsin) and mix with test tube
containing 5ml of milk solution.

3. Shack well and place each type of trypsin mixed with milk into the
various water bath with different temperature ranges.( So in each
water bath 1 calf trypsin and 1 fungal trypsin would be placed in)

4. Check every 5mins to check, and record the temperatures each time
you check. When the temperatures start to drop down remove the test

5. Repeat at least 3 times and record you final results, compare the
temperatures ranges for each trypsin and milk solution in various
water baths .

6. plot a graph of temperature against time and comment on your graph.


when the temperature start going down because it has denatured the
graph will look like this

Effect of temperature


1. Collins Advanced Science Biology by Mike Boyle and Kathryn Senior

2. Collins Advanced Modular Sciences Biology AS by Mike Bailey and
Keith Hirst

3. Longman (

4. Class notes Enzymes

5. Sci-Journal ( )
Return to