Solute Potential of Cell Sap of Plant Epidermal Cells

Solute Potential of Cell Sap of Plant Epidermal Cells

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Solute Potential of Cell Sap of Plant Epidermal Cells

Aim: To estimate the solute potential of a plant tissue.

Results:

Molarity of Solution

Plasmolysed Cells

Percentage of Cells That Were Plasmolysed

.3M

1/70

.01%

.4M

0/70

0%

.5M

5/70

7%

.6M

12/70

17%

.7M

29/70

41%

1.0M

56/70

80%

It must be taken into account, that the experiments procedure was
changed. This could have had a great affect on the results. The part
of the procedure that was changed was the time. Instead of the
epidermal cells being left in the solutions 20 minutes, they were left
for a day. Not only, were they left much longer, but the molarity of
the solutions could have also changed since they were left uncovered
for over 24 hours and some of the water could have been evaporated.
Another factor that contributed to the errors in this investigation
was that all of the data was approximated. Every single cell that
appeared in the microscope was not accounted for and therefore all
collected data is not exact, but instead a rough calculation. Another
error that could have occurred, and that would explain what happened
to the cells that were put in the solution with .4M, is that onion
skin dried out before it was placed in the sucrose water. Also, there
could have been a variation between the different onion epidermis
cells that were used.

Conclusion:

The results of the investigation show that the greater the molarity,
the more plasmolysed cells will appear. Plasmolyses is the shrinkage
of volume of a cell. This is caused by the falling of water
concentrations and ultimately results in the contraction of the
membrane. The most contracted membranes were found in epidermal cells
that were left in the solution with the greatest molarity; in this

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case it was the cells that were left in the solution with a 1.0
molarity. The graph shows a constant start. It very quickly shoots
up, with a huge leap from .6Mto 1.0M.

The results for this experiment did not come back exactly the way they
should have to prove the point. In the epidermal cells that were
placed in the .3M solution .01% of the cells plasmolysed, but in the
epidermal cells that were placed in the .4M solution 0% of the cells
were plasmolysed. If the experiment had gone exactly as planned, the
cells that were placed in the .4M solution would have had more than
.01% cells plasmolysed. However, in the overall view, the cells that
were placed in the .3M solution came back with .01% of the cells
plasmolysed and the cells that were placed in the 1.0M solution had
80% of the cells plasmolysed.

Some of the major inaccuracies that have to be mentioned about this
investigation are that part of the procedure that was changed was the
time. Instead of the epidermal cells being left in the solutions 20
minutes, they were left for a day. Not only, were they left much
longer, but the molarity of the solutions could have also changed
since they were left uncovered for over 24 hours and some of the water
could have been evaporated. Another factor that contributed to the
errors in this investigation was that all of the data was
approximated. Every single cell that appeared in the microscope was
not accounted for and therefore all collected data is not exact, but
instead a rough calculation. Another error that could have occurred,
and that would explain what happened to the cells that were put in the
solution with .4M, is that onion skin dried out before it was placed
in the sucrose water. Also, there could have been a variation between
the different onion epidermis cells that were used, because not all
cells in the same tissue have the same solute potential.

Next time, to avoid these errors, the correct time should be used.
The pieces of onions should be cut more exactly and each cell that is
visible through the microscope should be counted. To evade to
possibility of the onion cells drying out they should be immediately
placed into the sucrose solution. Also, during the waiting period the
solutions that the cells are in should be covered, as to avoid any
evaporation that may occur.
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