The Effect of Light Intensity on Photosynthesis Of Elodea Canadensis

The Effect of Light Intensity on Photosynthesis Of Elodea Canadensis

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The Effect of Light Intensity on Photosynthesis Of Elodea Canadensis

Introduction

I wanted to find out how much the light intensity affected the
Photosynthesis in Elodea Camadensa. I decided to do this by measuring
the amount of oxygen created during photosynthesis.

Photosynthesis is the procedure all plants go through to make food.
This process uses Carbon dioxide, water and light energy. It produces
Oxygen and Glucose.

[IMAGE]Carbon dioxide + water Light Energy glucose + oxygen

Chlorophyll

[IMAGE]6CO2 + 6H20 Light Energy C6 H12 O6 + 6O 2

Chlorophyll

Photosynthesis occurs in the leaves of the plant in the palisade
layer. Chlorophyll in the cells in the palisade layer absorb light for
photosynthesis. The plant releases the oxygen created in
photosynthesis back into the air but it uses or stores the glucose for
energy, respiration, growth and repair.

The leaves and plants are also specially adapted for photosynthesis in
their structure and cell alignment.

Preliminary Experiment

Apparatus

* Piece of Elodea Canadensis

* Bulb

* Voltmeter

* Test tube

* Beaker

* Box

* Two electrical wires

* Stopwatch

* Thermometer

Method

I put a piece of Elodea Canadensis (pond weed) in a test tube and
covered it with water.

The test tube was then placed in a beaker with a thermometer so that
the water stayed the same temperature, this was then placed in a
cardboard box with a bulb attached to a voltmeter by the electrical
wires so that I could concentrate how much light the plant got.

I varied the voltage (intensity) of the bulb and counted how many
bubbles of oxygen were created at each voltage in one minute.

I tested each voltage three times for accuracy for a minute each time.

Results

Preliminary Experiment

Amount of bubbles

Light intensity (lux)

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Related Searches


Volt (V)

Test 1

Test 2

Test 3

Average

1550

10

60

29

40

43

380

8

43

23

30

32

120

6

32

29

28

30

Evaluation

The table shows us that more oxygen was created at a higher light
intensity. I think that this experiment was not very reliable as the
way of counting the amount of oxygen was not reliable at all. If I was
to improve the experiment I would change the apparatus I used so that
I could measure the amount of oxygen given off accurately.

I would also test more voltages of light to widen the results of the
experiment, thus creating a clearer picture of how the light intensity
is affecting the plant. The preliminary experiment is unreliable due
to the simplicity of it.

Improved Experiment

Prediction

I predict that the rate of photosynthesis will increase until other
limiting factors run out.

Limiting factors are:

* Carbon Dioxide

* Temperature

* Amount of water

* Light Intensity

If we run out of Carbon dioxide, water or light photosynthesis cannot
take place. Also if the temperature is too high or too low
photosynthesis cannot take place either.

I believe my prediction to be correct as plants need sunlight to
photosynthesise. So if the light intensity increases so should the
rate of photosynthesis. If you look at a plants leaves surface area
and position on the stem you see that it is trying to get more light.
This shows us that the more light a plant gets the more it will
photosynthesise this all depends on the limiting factors. I will vary
the light intensity in the experiment. But I will control the amount
of water and temperature so that they are always the same.

Apparatus

* Beaker

* Test tube

* Piece of Elodea Canadensis

* Water

* Voltmeter

* Capillary tube

* Bulb

* Syringe

* Two electrical wires

* Box

* Clamp stand

I will, for safety reasons, keep all water away from the electrical
equipment and the bulb.

Method

I will place the piece of Elodea Canadensis and place it in the
syringe. Attach the capillary tube to the end of the syringe and fill
with water.

Place the syringe in a clamp stand and place the end of the capillary
tube in an empty beaker, this will then be placed in the cardboard
box. I will draw up some oxygen, using the syringe plunger, into the
capillary tube to about half way up, I will then mark where this comes
to with a pen on the capillary tube.

I will turn the bulb on by attaching it to the voltmeter by the
electrical wires.

I will vary the voltage of the bulb timing each volt for one minute. I
will mark the new position of the oxygen in the capillary tube and
measure how far it has moved down the tube. I will use this
measurement to calculate the amount of oxygen in mm2.

Results

Amount of oxygen (mm³ )

Test 1

Test 2

Test 3

Average

Voltage (V)

Light Intensity (lux)

3.14

3.95

1.57

2.88

4

21

4.71

3.14

2.36

3.4

5

103

3.14

2.36

1.57

2.36

6

190

3.14

3.14

2.36

2.88

7

346

1.57

1.96

2.36

1.96

8

422

1.57

1.57

3.14

2.09

9

680

3.53

2.36

3.95

3.27

10

1485

5.5

3.95

3.95

4.45

11

1770

10.6

11

11

10.87

12

1960

14.14

12.57

10.21

11

13

6660

Evaluation

I think that this experiment is much more accurate than the
preliminary experiment, due to the fact that the preliminary
experiment was very simple.

It shows that the higher the light intensity is the more oxygen is
produced. I do have a couple of anomalies which I believe to have
occurred because the experiment was run over two separate lessons, so
the pond weed/equipment was different in each lesson. Some examples of
these anomalies are volts 4, 5 and 8.

Otherwise I think that this experiment is more reliable and gives us a
clearer picture than the preliminary experiment.

Conclusion

I have realised in this experiment that without the products of
photosynthesis all living organisms would die, including plants as
eventually the limiting factors would run out such as carbon dioxide.
Both these experiments tell us how much oxygen is produced during
photosynthesis by using similar but different methods. Both
experiments came up with valuable results even though neither where
completely accurate. The improved experiment results showed a wider
range of volts and accuracy therefore being the more reliable source
of results.

I think that there must be a more reliable way of doing these
experiments but with the equipment and time we had I believe that this
was the most accurate and reliable way of doing these experiments.

If I was to perform a third experiment which was yet again improved I
would test a larger range of volts and find a more reliable way of
doing the experiment on a whole.

We have proved that oxygen is a product of photosynthesis and recorded
reasonable results showing the amount of oxygen produced. I have also
included graphs using the information in the tables. These show the
rate that oxygen was produced (rate of photosynthesis).

I think that this experiment was a good investigation into
photosynthesis and shows us about production rate of oxygen.
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