The Effects of Sucrose Molarity on Cells in the Stem Tuber of a Potato

The Effects of Sucrose Molarity on Cells in the Stem Tuber of a Potato

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The Effects of Sucrose Molarity on Cells in the Stem Tuber of a Potato

Planning

In this investigation I am trying to find out what molarity of sucrose
solution is same as the molarity of sucrose in the cells of a potato.
In this experiment I am going to change the molarity of the sucrose
solution out the cells of potato.

I predict that if the molarity of sucrose solution outside the cells
is isotonic to the molarity of the sucrose inside the cells then there
will be no change in weight of the potato disks. I predict this
because if the molarities are isotonic inside and outside the cells
then there will be a balanced osmotic movement of the water molecules
from inside and outside hence there will be no change in weight. I
also predict that if the molarity of the sucrose solution is
hypertonic outside the cell than the inside then there will be loss in
weight because osmosis is the movement of water molecules from an area
of high concentration of water molecules to an area low concentration
of water molecules and if there is there is a higher concentration of
sucrose solution outside then the water molecules will diffuse outside
the cell, which suggests that there will be loss in weight. Vice versa
if there is hypotonic concentration of sucrose solution outside the
cells compared to inside then there will be gain in weight.

Sucrose Molecules




[IMAGE]

[IMAGE]




Text Box: Water Molecules [IMAGE]


Below is the method that I am going to follow through my preliminary
investigation.

1. I will take a potato and cut it into half

2. Using a hole borer I will then take one cylinder of potato tissue
from the potato.

3. I will then cut the cylinder into four disks and I will then
weigh them using an electronic balance. In order to get accurate
weights I will weigh the filter paper and the cell sap on it with

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the potato disk and I will then subtract the weight of the potato
disk from the total reading.

4. I will then put the potato disks into different tonicity of
sucrose solutions.

5. I will then leave the disks overnight in the solutions and weigh
them again the next day and see the change in the weight.

I will be using the following the equipments.

Ø A knife

Ø A tile

Ø 2* pipettes

Ø Measuring Cylinder

Ø Potato

Ø Hole borer

Ø Electronic Balance

Ø Ruler

Ø Different tonicity of sucrose solution.

Ø 4* specimen jars

In order to obtain reliable results I will use the above equipments
accurately and precisely.

I will make this investigation valid by using the right equipment and
by trying and keeping the other possible variables that like the
volume of the solution, time- how long I am going to keep the disk in
the solution, temperature, and type of potato constant through out the
experiment because these variables could affect the process of osmosis
in the potato cells

In this experiment use of the equipment is really important and it
could affect the results I get. For example if I was supposed to use a
blunt knife to cut the potato disks, there is a huge chance that I
will rupture the cell membranes of the potato. The ruptured cells of
the potato will not take part osmosis Therefore I will be very careful
in cutting the disks.

Below is the results table of my preliminary investigation.

Molarity of sucrose solution (M)

Weight of disks before (g)

Weight of disks after (g)

% change in weight

0

0.24

0.28

+16.67

0.24

0.28

+16.67

1

0.24

0.16

-33.3

0.24

0.15

-37.5

Now that I know that the disks of potato has gained weight in the 0
molar sucrose solution and they have lost weight in the 1 molar
sucrose solution I have decided that my range of the molarity of the
sucrose solution will be between 0 and 1 molar because I want to find
out at which molarity of sucrose solution the potato's weight is
unchanged.

Analysis

Although I tried and cut the disks very accurately and made sure that
they are of same weight I realise that to make my results more
reliable I will find out the percentage change in weight for each of
the potato disk. I am going to do this by using the below formula. As
I look at the results I obtained and the graph I have plotted, I found
that as I increased the molarity of the sucrose solution there was a
decrease in % weight gain of the potato disks.

Change * 100

Original

After I have calculated the percentage change in weight I will then
plot a line graph of sucrose concentration against percentage change
in mass. I will also draw a curve of best fit for this graph.

As I can see from my graph there is pattern in my results. It shows
zero change at first and then as I continue to increase the molarity
of sucrose solution the percentage in weight is a loss which gets
bigger. My graph also shows that after 1 molar of sucrose solution the
graph appears to be levelling. This is happens because a point comes
when there is no water left in the vacuole because the cell loses it
due to osmotic movement of the water out. This causes the protoplast
to pull away from the cell wall and the protoplast no longer presses
on the cell wall. This point is known as incipient plasmolysis. Any
further loss of water causes the protoplast to shrink more and
eventually the vacuole disappears completely. This condition is called
plasmolysis and the cell is said to be flaccid because there is no
water left in the cells.

My conclusion does support my prediction where I say that if the
concentration of the sucrose solution is hypertonic outside the cell
there will be decrease in weight and vice versa if the concentration
of the sucrose solution is hypotonic outside the cell there will
increase in weight of the cell and if the tonicity of the solution is
isotonic at both sides then there will be no change in weight and like
wise my results support my prediction too

Evaluation

The procedure that I have used to obtain the results is quite straight
forward yet accurate. I think the results I obtained are reliable and
accurate as there are not many anomalous points in my plotted graph
and they fit the pattern very closely. I think that this investigation
was carried on fair grounds and I tried and kept the controllable
variable constant. To support my conclusion I have collected enough
evidence, I have collected three readings for each sucrose solution
and I have got an average for them. If I was to repeat this experiment
again I will use different types of potato for my investigation. This
will help me in understanding the osmotic movement in different types
of potato.
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