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To investigate the effects that different concentrations of sodium
hydrogen carbonate on the rate of photosynthesis.
Light energy & chlorophyll
[IMAGE]6CO2 + 6H2O C6H12O6 + 6O2
The resistance of a wire depends on certain factors. Some of these
variables are listed below:
· Light Wavelength
· Carbon Dioxide (sodium hydrogen carbonated)
All these factors will have to be kept constant except the
concentration of sodium hydrogen carbonate to make sure that the
investigation is a fair test. I have decided to investigate how the
concentration of sodium hydrogen carbonate affects photosynthesis
because other factors such as temperature and wavelength are hard to
control or vary. I will weaken the concentration by half each time and
will start at 5% and make my way down to 0.08 %.
I Prediction: I predict that decreasing the concentration will
decrease the rate of photosynthesis at a proportional rate until it
stops completely. This is because when you take away C02, you are
reducing the amount of resources the plant has to work with so there
should be less oxygen being produced.
To make sure that my test is fair I will make sure to keep all the
factors mentioned above the same apart from the concentration of
sodium hydrogen carbonate. I will make sure they stay the same by
checking them before each test. I will also do each test three times
and make a mean average. I must also thoroughly wash all equipment,
after every test.
· Pond weed
· Measuring cylinder
· Sodium hydrogen carbonate (NaHCO3)
Risk Assessment and Safety Precautions
There are not many safety precautions that need to be taken into
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"Investigating the Effects of Sodium Concentrations Have on the Rate of Photosynthesis." 123HelpMe.com. 24 Mar 2019
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· Do not get any chemicals on skin or in your mouth as this could be
· Make sure to keep any electrical wires from the lamp away from
water, thus preventing an electrical shock
* Collect the apparatus.
* Measure 7cm of pond weed; make sure there is a clean cut for the
oxygen to come out.
* Put the pondweed in the boiling tube, when the boiling tube is in
* Get the solution of 5% Sodium Hydrogen Carbonate (NaHCO3). Put
this in the boiling tube
* Put the lamp on and shine it at the boiling tube.
* Time it for one minute, to let the pondweed start to
* Then count the number of bubbles of oxygen, for one minute.
* Do this three times to get an average.
* Clean the boiling tube and mix up a solution of 2.5% NaHCO3.
* Do from step 4 but with 2.5% NaHCO3 then with 1.25%, 0.63%, 0.31%,
0.16% and 0.08% (all to 2 d.p).
Here is my results table
Concentration of NaHCO3
Tests (no. Oxygen bubbles)
% To 2 d.p
Average (to 2 d.p)
My graph shows me that as the concentration of the Sodium Hydrogen
Carbonate increases so does the amount of Oxygen given off. This is
because there is more Carbon Dioxide so the pondweed has more
resources to use for photosynthesis.
I do not think that my investigation was very fair because all of the
bubbles were different sizes. This made our results inaccurate because
we could not measure how much Oxygen there was. The only anomalous
result on my graph is the result for 5 % concentration, which is
higher than all of the other results. I think this can be explained
because the NaHCO3 is twice as big as the next result, which means it
should be correct to stand out. My results support my prediction of
the number of bubbles getting smaller as the concentration goes down.
To further investigate, I could use more concentrations of NaHCO3
going even as high as 100%.