The Effect of the Enzyme Catalase on Hydrogen Peroxide When the Temperature is Changed
Plan:
Chemical reactions take place in all living cells. All of these
reactions are enzyme-driven. Some of these reactions produce
by-products. One by-product is Hydrogen Peroxide (H2O2). It is a
poison and must be removed from the cells. The cell produces an enzyme
called Catalase to break down the Hydrogen Peroxide into water and
oxygen. This experiment will investigate the effect of temperature on
the rate of the breaking down of Hydrogen Peroxide by Catalase. I will
do this by measuring how much oxygen is produced when Catalase and
Hydrogen Peroxide react.
I predict that as the temperature increases, the rate of the reaction
will increase. I think that when the temperature is doubled, so the
reaction rate will also double. This is due to the particle theory. As
the temperature rises, the particles move faster and collide more
often. This gives the "lock and key" reaction more chance to occur.
Therefore, as it is a temperature coefficient with a Q10 of 2, if the
temperature is raised by 10ºC, the particles will move doubly fast,
collide twice as often and the rate of reaction will double. There
will be a point when this relationship isn't true. This will be at
around 60ºC when the enzyme denatures.
To make this a fair test, we need to keep the same all variables that
could affect the outcome except for the one being tested, temperature.
So the pH, amount of solutions used, concentration of solutions,
apparatus etc. must all remain the same.
As my source of Catalase, I will use potato. This is easier to cut and
measure than other sources such as liver, which would be hard to cut
into equal pieces. The potato "chip" will be pre-warmed in a boiling
tube in a water bath of each temperature for 10 minutes, as will 20cm3
of H2O2. Once the desired temperature is reached, the chip and H2O2
will be mixed in the boiling tube, shook a couple of times and placed
Investigation of How the Concentration of Catalase Enzyme Affects the Rate of Reaction Aim: To find out how the concentration of Catalase Enzyme will affect the enzyme activity and the rate of reaction towards Hydrogen Peroxide. (H O ) Prediction: I predict that with the higher concentration of enzyme, the likelihood of it breaking down molecules will be greater because there will be more enzymes to work at the substrate and the chances of it colliding will be higher making the activity time quicker. Equipment: · Syringe · Measuring Cylinder (×2) · Knife · Blender · Beakers (×2) · Balance · Hydrochloric Acid in a beaker · Stop clock · Potato · Water in a beaker Preliminary Experiment: In this experiment we will be using an enzyme called Catalase. By using different amounts of this enzyme we will be diluting it with water to test how the concentration of Catalase affects the rate of reaction with Hydrogen Peroxide.
The Effect of Temperature on the Rate of Respiration in Yeast There are two types of respiration in yeast: Aerobic: [IMAGE] Anaerobic: Glucose [IMAGE] Carbon dioxide + ethanol + energy Respiration is controlled by enzymes, which are proteins which speed up one or more biological reactions. Within any cell many chemical reactions are going on at any one time. Yeast has many different types of enzymes that speed up respiration. Prediction I predict that as temperature increases, the rate will also increase, until a certain optimum temperature, after which, the rate will decrease until the rate is zero as respiration has stopped completely. Reason
The Effect of pH on the Activity of Catalase Planning Experimental Work Secondary Resources Catalase is a type of enzyme found in different types of foods such as potatoes, apples and livers. It speeds up the disintegration of hydrogen peroxide into water because of the molecule of hydrogen peroxide (H2O2) but it remains unchanged at the end of the reaction.
The Effect of Temperature on the Action of Peroxidase Enzyme Aim To find the effect of temperature on an enzyme in this case peroxidase, by studying it decomposing hydrogen peroxide. Planning I am measuring the amount of gas given off in the reaction. By collecting this figure I can determine the rate of reaction. If I know the rate of reaction I can find out what temperature peroxidase works best at. Equipment · Trough · Clamp · 100ml measuring cylinder · Stop clock · 10ml measuring cylinder · Delivery tubes · Bung · Conical flask Variables Change
The Effect of Changing the Concentration of the Enzyme Catalyst on the Rate of Reaction on Hydrogen Peroxide
Investigating the Effect of Substrate Concentration on Catalase Reaction. Planning -Aim : The aim of the experiment is to examine how the concentration of the substrate (Hydrogen Peroxide, H2O2) affects the rate of reaction. the enzyme (catalase).
Temperature's Effect on the Production of Oxygen From Yeast and Hydrogen Peroxide Planning Aim --- My aim is to discover how temperature effects the production of oxygen from yeast and hydrogen peroxide. I will mix yeast with hydrogen peroxide and use an upturned measuring cylinder to measure how much oxygen is produced. To find out how much hydrogen peroxide and yeast I will use, I will carry out pre-tests. This will also aid accuracy of the final experiment by uncovering potential flaws in the method. Hypothesis and Theory
Investigate the Effect of pH on Immobilised Yeast Cells on the Breakdown of Hydrogen Peroxide
Investigating Factors that Affect the Rate of Catalase Action Investigation into the factors which affect the rate of catalase action. Planning Aim: To investigate the affect of concentration of the enzyme catalase on the decomposition reaction of hydrogen peroxide. The enzyme: Catalase is an enzyme found within the cells of many different plants and animals. In this case, it is found in celery.
The Effect of Temperature on the Activity of the Enzyme Catalase Introduction: The catalase is added to hydrogen peroxide (H²0²), a vigorous reaction occurs and oxygen gas is evolved. This experiment investigates the effect of temperature on the rate at which the enzyme works by measuring the amount of oxygen evolved over a period of time. The experiment was carried out varying the temperature and recording the results. It was then repeated but we removed the catalase (potato) and added Lead Nitrate in its place, we again tested this experiment at two different temperatures and recorded the results. Once all the experiments were calculated, comparisons against two other groups were recorded.
What Affects the Rate of Breakdown of Hydrogen Peroxide by Enzymes Aim = == The aim of this experiment is to find out how temperature and concentration affect the breakdown of hydrogen peroxide by an enzyme (yeast). I hope to achieve reliable results that will confirm my predictions.
The Effect of Temperature on the Rate of Respiration in Yeast I have chosen to investigate the affect temperature has on the rate of respiration in yeast. I will use an experiment to determine whether the yeast's rate of respiration will be quicker, slower or if it does not change when the temperature is varied. Scientific Knowledge The first thing to say about enzymes is that they are proteins and they are found in all types of organisms from humans to viruses.
The pH of the solution would alter the rate of the reaction if it was
How the Concentration of the Substrate Affects the Reaction in the Catalase Inside Potato Cells
Investigating the Effect of the Enzyme Catalyse On Hydrogen Peroxide Introduction The aim of this experiment is to determine the effects of varying enzyme (catalyse) on Hydrogen Peroxide. Hydrogen Peroxide + Catalyse à Water + Oxygen 2H2O2 à H2O + O2 + Heat Apparatus & Diagram [IMAGE][IMAGE][IMAGE][IMAGE][IMAGE][IMAGE][IMAGE] Bung Potato Hydrogen Peroxide Water Collected Oxygen Delivery Tube Measuring Cylinder [IMAGE] Using the Equipment Safely It is important that we use the apparatus carefully, as safety will be an issue throughout the whole experiment. We will wear goggles and an apron or lab coat to protect our eyes and clothes. As we are using enzymes and Hydrogen Peroxide we need to be extra careful, ensuring they don't come into contact with our eyes, skin or clothes. Catalyse is an enzyme found in all living cells.