The Effect of Concentration of Sucrose Solution on Osmosis in Potato Tissue

The Effect of Concentration of Sucrose Solution on Osmosis in Potato Tissue

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The Effect of Concentration of Sucrose Solution on Osmosis in Potato Tissue

Aim:-

To find the effect of varying external concentration of sucrose
solution on osmosis in potato tissue.

Variables:-

J The concentration of the sucrose solution around the potato tissue.
Depending on the concentration of the solution, osmosis will occur
more or less.

J The size and shape of the potato. The longer and wider the potato
tissue, the larger the surface area, which means that more of the
potato is exposed to the solution giving it more of a chance of
osmosis occurring.

J The condition of the potato being used. If the potato is rotting or
rotten, then osmosis might not occur properly as the cells may be
dying or dead.

J The volume of the solution. If there is more of the solution, then
the potato will have more for it to use in osmosis.

J The temperature. If it is hot, then some of the solution is likely
to evaporate, leaving sugar behind, which will not be used in the
osmosis.

J Time that the experiment is left for. The longer that the experiment
is left for, the more of a chance osmosis has to happen.

J Light condition. If the light is too intense, it may kill some cells
by dehydrating them, effecting osmosis.

Apparatus:-

¥ 2fairly large potatoes

¥ A cork borer

¥ A stick

¥ A tile

¥ Paper towels

¥ A razor/blade

¥ Electric scales

¥ 5 syringes

¥ Ruler

¥ A watch/clock

¥ Solutions-

[IMAGE]

¥ 10 test tubes

¥ Test tube rack

¥ 10 bungs

¥ A dark cupboard

¥ Thin marker pen.

Fair test table :-

Factor

experiment

Concentration of sucrose solution. ( M )

Size, shape and volume of potato.

Condition of potato.

Volume of solution.

(ml)

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Temperature in the lab. ( degrees Celsius)

Time experiment is left for. (mins)

Light intensity

1

0.00

6mm long cork borer cylinder

fresh

20

16

300

Dark

2

0.25

6mm long cork borer cylinder

fresh

20

16

300

Dark

3

0.50

6mm long cork borer cylinder

fresh

20

16

300

Dark

4

0.75

6mm long cork borer cylinder

fresh

20

16

300

Dark

5

1.00

6mm long cork borer cylinder

fresh

20

16

300

Dark

Method :-

¥ Firstly, hold down the potato on the tile and using the cork borer,
care fully cut 5 cylinders out of each potato, using the stick, push
the potato out of the cork borer.

¥ Being careful, use the razor/blade, to cut the potato cylinders to
60mm each, making sure that there is no skin on the potato.

¥ Weigh each of the potato cylinders, and note the results.

¥ Arrange the test tubes in the racks.

¥ Label the tubes with the concentration of the solution, use the same
concentration twice.

¥ Use the syringes to fill the test tubes with 20ml of each
concentration of solution.

¥ Make sure the cylinders are blotted dry with paper towels.

¥ Put one potato cylinder in each of the tubes and put a bung in each
one.

¥ Leave for 300 minutes (5 hours)

¥ Empty the solutions from the test tubes and then keeping them in
order, dry them with some paper towels.

¥ Again, weigh the potato cylinders and note the results.

I chose to change the varying concentration to more of a selection
than in my preliminary work, because then the results would be more
accurate.

Also, I chose to change the factor I would measure from my preliminary
work, because the results of it showed that there was not much of a
change in the length.

Prediction :-

Osmosis is the diffusion of water from a high concentration, dilute
solution to a lower concentration, more concentrated solution, through
a partially permeable surface.

Looking at this, I predict that as the concentration of the sugar
solution increases, the weight of the potato cylinder will decrease.
This would be because as the potato cylinder cells have a greater
amount of water than the strong sugar solution outside, the water
would diffuse from the potato cells, to the solution outside of it,
meaning it loses water meaning it loses weight. So a potato cylinder
in a 1.00M solution, the potato cylinder will lose mass and as the
concentration decreases, then the potato cylinder will have a greater
mass, and will have gained mass in a 0.00M solution.

Results :-

Concentration of solution. (M)

Mass before.

(g)

Mass after.

(g)

Difference in mass. (g)

0.00

5.22

5.73

+ 0.51

0.00

5.03

5.50

+ 0.47

0.25

5.10

5.45

+0 .35

0.25

5.15

5.44

+ 0.29

0.50

5.07

4.98

- 0.09

0.50

5.17

5.03

- 0.14

0.75

5.24

4.80

- 0.44

0.75

5.25

4.79

- 0.46

1.00

5.03

4.27

- 0.76

1.00

5.12

4.41

- 0.71

Calculations :-

Concentration of sugar solution. (M)

Change in mass.

(%)

Average change in mass. (%)

0.00

0.00

+ 9.77

+ 9.34

+ 9.56

0.25

0.25

+ 6.86

+ 5.63

+ 6.25

0.50

0.50

- 1.78

- 2.71

- 2.25

0.75

0.75

- 8.40

- 8.76

- 8.58

1.00

1.00

- 15.11

- 13.87

- 14.49

Analysis.

Osmosis is the diffusion of water from a high concentration, dilute
solution to a lower concentration, more concentrated solution, through
a partially permeable surface.

Looking at this, I predict that as the concentration of the sugar
solution increases, the weight of the potato cylinder will decrease.
This would be because as the potato cylinder cells have a greater
amount of water than the strong sugar solution outside, the water
would diffuse from the potato cells, to the solution outside of it,
meaning it loses water meaning it loses weight.

So a potato cylinder in a 1.00M solution, the potato cylinder will
lose mass and as the concentration decreases, then the potato cylinder
will have a greater mass, and will have gained mass in a 0.00M
solution.

This is my prediction and I was correct in saying that is what would
happen.
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