The Effect of Temperature on the Rate of Respiration in Yeast

The Effect of Temperature on the Rate of Respiration in Yeast

Length: 1232 words (3.5 double-spaced pages)

Rating: Excellent

Open Document

Essay Preview

More ↓
The Effect of Temperature on the Rate of Respiration in Yeast
I have chosen to investigate the affect temperature has on the rate of
respiration in yeast. I will use an experiment to determine whether
the yeast's rate of respiration will be quicker, slower or if it does
not change when the temperature is varied.

Scientific Knowledge


The first thing to say about enzymes is that they are proteins and
they are found in all types of organisms from humans to viruses. They
function in the body as catalysts. In other words they speed up the
rate of chemical reactions in the body. Enzymes actually accelerate
chemical reactions by a factor of about 1 million. Enzymes are organic
catalysts. Without enzymes the metabolism of an organism would be too
slow for the organism to survive. The word enzyme actually means "in
yeast" as they were first discovered in these micro-organisms.
----------------------------------------------------------------------


Enzymes are found inside and outside cells. Enzymes found outside
cells are for example, those that control metabolism while enzymes
found inside cells for example, gut digestive juices and the enzymes
secreted by bacteria, which digest their food outside the body then
reabsorb the products. Enzymes work on particular organic chemicals,
these are called substrates. So food is the substrate of digestive
enzymes.
--------------------------------------------------------------------


The lock and key hypothesis attempts to explain how enzymes are
specific to particular substrates and how they may work. In this
hypothesis the enzyme is the lock and the substrate(s) is the key.
Enzyme molecules have a particular shape like a lock and only a
particular substrate (key) can fit into that lock. The part of the
enzyme that binds the substrate is called the "active site". Just like
other catalysts, after the enzyme has been involved in the reaction it

How to Cite this Page

MLA Citation:
"The Effect of Temperature on the Rate of Respiration in Yeast." 123HelpMe.com. 31 Mar 2020
    <https://www.123helpme.com/view.asp?id=120937>.

Need Writing Help?

Get feedback on grammar, clarity, concision and logic instantly.

Check your paper »

The Effect of Temperature on the Rate of Respiration in Yeast Essay

- The Effect of Temperature on the Rate of Respiration in Yeast Preliminary Experiment Aim To investigate the time it takes for the yeast in glucose suspension to reach the same temperature as the water it is placed in. Apparatus ========= · Stopwatch · Plastercine · 2 Thermometers · Ice Cream Tub · Kettle · Syringe Method ====== · First I will fill up the ice cream tub with water at one of the temperatures I would be using for the main experiment (80degrees will be tested) · Next I will check the temperature of the yeast in the syringe and record it....   [tags: Papers]

Free Essays
1588 words (4.5 pages)

Essay on The Effect of Temperature on the Rate of Respiration in Yeast

- The Effect of Temperature on the Rate of Respiration in Yeast I have chosen to investigate the affect temperature has on the rate of respiration in yeast. I will use an experiment to determine whether the yeast's rate of respiration will be quicker, slower or if it does not change when the temperature is varied. Scientific Knowledge The first thing to say about enzymes is that they are proteins and they are found in all types of organisms from humans to viruses....   [tags: Papers]

Free Essays
1232 words (3.5 pages)

Investigation Into the Effect of Temperature On the Rate of Respiration of Yeast

- Investigation Into the Effect of Temperature On the Rate of Respiration of Yeast Preliminary Work For my preliminary work, I am working with 35ml of yeast. I think that this is the best volume to use as it is about ¾ of a test tube full, and it allows for the yeasts expansion when heated. I am trying to find out the best range of temperatures to be used in finding out the respiration of the yeast, and I am also trying to find an equilibration time that can be used in the main experiment, as the time taken for the yeast to heat up to the desired temperature....   [tags: Papers]

Free Essays
1314 words (3.8 pages)

The Effect on the Rate of Respiration of Yeast Cells with Glucose when the Temperature is Varied

- The Effect on the Rate of Respiration of Yeast Cells with Glucose when the Temperature is Varied Aim The aim of the experiment is to investigate the effect of temperature on the rate of respiration of yeast cells with glucose. As yeast cells use up glucose in respiration, carbon dioxide gas is given off. Measurements of the volume of carbon dioxide gas given off within a set amount of time can be used to measure the rate of reaction. A fast rate of reaction would be indicated by a large volume of carbon dioxide gas being collected within this set amount of time....   [tags: Papers]

Research Papers
4673 words (13.4 pages)

Essay on The Effect of Temperature on Yeast Respiration

- The Effect of Temperature on Yeast Respiration PLAN Aim: To determine the effect of temperature upon respiration of Yeast. Prediction: I predict that the respiratory rate of the yeast will increase in speed as the temperature of the water increases. However, it may reach a peak, and slowly decline as the temperature increases further. My reason for the above prediction is that, anyone who has cooked, knows that yeast is supposed to respond in warm water, so I believe that the warmer the water, the more bubbles of CO² produced....   [tags: Papers]

Free Essays
1406 words (4 pages)

Lab Experiment: The Effect of Temperature and Respiration Essay

- Introduction: Respiration commonly known as the inhalation and exhaling or breathing has a more little known definition. This is the definition that involves the cellular level of eukaryotic cells. Cellular respiration may best be described by the following equation: C6h1206+602-6CO2+6H20+36ATP. ATP that is the energy needed for a cell to function as part of cellular respiration. ATP is needed to power the cell processes. If cells are denied energy they will die. The second law of thermal dynamics says energy is lost in the form of heat whenever energy changes form....   [tags: essays research papers]

Free Essays
627 words (1.8 pages)

Temperature and Respiration in Crickets Essay

- Temperature and Respiration in Crickets Planning I am going to investigate respiration in crickets and how temperature varies the rate of respiration. The calculation for aerobic respiration is: [IMAGE]Oxygen + Glucose Carbon dioxide + water + energy [IMAGE]6O2 + C6H12O6 6CO2 +6H2O + (J) I will do the experiment safely by making sure that the water will not be too hot or too cold, this is a safety precaution for me as well as the crickets....   [tags: Papers]

Free Essays
1494 words (4.3 pages)

The Effect Of Temperature On The Metabolic Rate Of An Animal At Rest Under Normal Conditions

- SANA MARRYUM METABOLISM IN ECTOTHERMS AND ENDOTHERMS; EFFECTS OF TEMPERATURE BIO 207 SECTION 4SV3 DECEMBER 7, 2014 LAB INSTRUCTOR YIJING SHAN MARRYUM 02 INTRODUCTION All living organisms require energy to perform their daily life activities. This energy is obtained in the form of ATP by a number of chemical reactions that results in breakdown or building up of different molecules. The sum of all the enzyme-catalyzed reactions inside the body is termed as metabolism. All these reactions are very important in maintaining the physiological functions of the body....   [tags: Metabolism, Energy, Temperature]

Research Papers
2597 words (7.4 pages)

The Effect of Temperature on Anaerobic Respiration of Yeast Essay

- The Effect of Temperature on Anaerobic Respiration of Yeast We wanted to find the effect of temperature on anaerobic respiration of yeast. We investigated how a mixture of yeast, water, sugar, and flour were affected. Diagram ======= Apparatus ========= 1g of yeast 0.5g of sugar 10g of flour 6 measuring cylinders - to put the sachets in and the 20cm3 of water Measuring cylinders to measure the water Thermometers - to keep the same temperature in the water baths Water baths - at 20ºC, 30ºC, 40ºC, 50ºC, 60ºC Hot air oven - at 70ºC Beakers - to use to put the yeast mixture in the cylinders....   [tags: Papers]

Research Papers
1044 words (3 pages)

Investigating the Effects of Temperature on the Rate of Respiration of Blowfly Larvae

- Investigating the Effects of Temperature on the Rate of Respiration of Blowfly Larvae This experiment was setup to investigate the effects of temperature on the rate of respiration of blowfly larvae. Background Knowledge The maggots involved in this investigation were blowflies at the larval stage of development, and they breathe through spiracles in the anterior and posterior segments. The respiratory tracts are not as developed at this stage, as the organism is not capable of flight, and so it respires less oxygen than an adult blowfly....   [tags: Papers]

Research Papers
1385 words (4 pages)

Related Searches

is not altered.
----------------------------------------------------------------------


However, heat can denature the enzymes causing them to lose their
shape so the "key" will not fit in the "lock". When this happens
respiration will slow down due to glucose being produced at a slower
rate. We can tells this has happened in the experiment because less
carbon dioxide is produced which is a bi-product of respiration.
--------------------------------------------------------------------


Glucose + Oxygen [IMAGE] Carbon dioxide + water + energy
--------------------------------------------------------


The normal temperature that enzymes in the human body work most
efficiently is 36 degrees Celsius, body temperature, so in my
experiment I want to try out the effects of temperatures higher and
lower than 36 degrees Celsius.
-------------------------------------------------------------------


The diagram below shows the lock and key hypothesis
---------------------------------------------------

Enzyme (lock)


Substrate (key)

Active site

Enzyme- substrate complex

[IMAGE]

Pilot Experiment

Prior to this investigation I carried out a pilot experiment of the
same kind but just using the temperatures of 20, 30 and 40 degrees
Celsius. Although this was a very basic experiment I found that I
needed to leave the yeast acclimatise for 5 minutes before recording
the results, which in the end gave more reliable and accurate results

Method and variables

There are many factors (variables) that could affect my investigation.
I need to keep all but one variable the same. I will vary the
temperature because I am investigating the effect of temperature on
the respiration of the yeast. So I need to keep variables such as
light the same as more light means faster respiration, the same amount
of glucose because more glucose means faster respiration. The amount
of yeast will also have an effect on the results, as if we are to use
different amounts in each experiment it will form an unfair test. I
must also make sure to leave all experiments undisturbed during the
course of respiring, this means they cannot be stirred or let air get
to them.

The basic method for my experiment is as follows:

I will change the temperature of the water surrounding the yeast by 5
degrees Celsius each time.

I will measure the number of bubbles produced by the yeast in a 5
minute period (having an additional 5 minutes at the start for the
yeast to adjust to the temperature change)

I will keep the amount of light and glucose the same and ensure to use
all the same apparatus in each experiment.

I will keep the mass of yeast used in each experiment the same.

The apparatus I will use is shown in the diagram below:

CO2 bubble

Test tube

Water heated to desired temperature

Yeast suspension

Test tube

100ml beaker

bung

Cold distilled water

Delivery tube

[IMAGE]

The full method I will be using is as follows:

The apparatus will be set up as shown above. The test tube with the
yeast suspension will be placed in a 100ml beaker. The beaker will be
filled ¾ of the way to the top with water. This water will have
already been heated to the desired temperature. It is the water in the
beaker that will provide the heat to the yeast. The test tube will
have a bung covering the end (to stop any C02 from escaping which
would ruin the experiment). A delivery tube with will carry the C02
produced by the yeast respiring to another test tube filled ¾ of the
way to the top with water. The end of the delivery tube will be kept
under the water so any C02 given of by the yeast will be shown
underwater as a bubble. I will count the number of bubbles given off
by the yeast and record the results in a cumulative frequency table.
This will then indicate which temperature the yeast respired the
quickest.

Each experiment will be repeated a second time so as to find any
anomalous results and if any are found that experiment will be
repeated once more to verify the correct result.

Safety

When dealing with yeast and alcohol I must make sure not to inhale or
consume in any way anything during the experiment as a lot of it may
be harmful in someway. Also if any glass is broken for any reason it
must be cleared up properly and safely.

Prediction

I predict that the yeast's respiratory rate of reaction should
increase in speed as the temperature increases. However this may at
some point peak and therefore cease to increase in speed, and decrease
due to the enzymes in the yeast being denatured. I have formed this
prediction because of my scientific knowledge of the "lock and key
hypothesis."

Analysis

Form the graphs what I have found out was that on the comparison of
volume of CO2 produced of different concentration of sugar solution
the concentration that has the most CO2 produced is 100% and the least
is 200%. This is because that supposing the more concentrated the
solution is the more ethanol and CO2 will be produced because more can
be react with the enzymes. But in fact it is not because the osmotic
pressure which is that the water will flow from less concentrated
solution into more concentrated solution. This result for the yeast
cells to loose water to the solution, which makes the yeast less
efficient

For the rate of fermentation the one that has the fastest rate is 100%
and the least is 200%. What I could also noticed from this graph is
that the rate increases proportionally as concentration increase. The
rate of reaction, in this case, is the movement of the meniscus per
minute. The rate of reaction depended on the surrounding temperature
and the concentration of the solution therefore the more concentrated
the solution is the faster the rate. For this graph though it is not
so accurate because that the rate in the beginning of the experiment
is ever so fast that it changes the look of the graph so much.
Therefore I ignore the first volume of the result because if I have
included it into the line the gradient of the graph will not be
accurate enough and this will result in the change of the rate of
reaction. The rate of reaction table could be seen in the obtaining
evidence along with the other results. The formula for calculating the
rate of the reaction is::

The change in volume of carbon dioxide (y)

The change in time per minute (x)

It is a shame that I did not have enough time to do the rest of the
experiment such as the sugar concentration of 50% because I do not
have enough time to complete it. Though I have not done the other
experiments I still have enough of the results to conclude the
experiment.

Although I did not do 50% sugar concentration I could still say that
the best sugar concentration will still be 100% because it has the
nearest osmotic pressure on both the solution and the yeast cells. The
main conclusion here is that the most important factor is the osmotic
pressure that effects the whole experiment:

Due to my prediction what I have predicted was nearly all right except
on which was that the amount of carbon dioxide given off will increase
as the concentration of sugar increase was wrong because that it is
exactly the opposite. Except that the other prediction is the same as
the result.
Return to 123HelpMe.com