The Effect of Temperature on the Rate of Reaction of Yeast

The Effect of Temperature on the Rate of Reaction of Yeast

Length: 1309 words (3.7 double-spaced pages)

Rating: Excellent

Open Document

Essay Preview

More ↓
The Effect of Temperature on the Rate of Reaction of Yeast

Aim

My aim is to view the effects of different temperatures on the rate of
respiration of yeast in a glucose solution. I will do this by
measuring the rate of which carbon dioxide is given off (CO2) by the
yeast.



Scientific knowledge
====================

From previous experiments I have learnt several things about yeast and
enzymes. I have learnt that an enzyme is a natural catalyst,
(something that speeds up the experiment without altering the out
come.) Yeast is a fungus that is used in fermentation this is because
it respires anaerobically and aerobically, the anaerobic respiration
is the useful bit in fermentation as it produces ethanol (alcohol).
The sucrose solution that the yeast is put in is needed as energy so
that the enzymes in yeast can respire. The equation is:

[IMAGE]Glucose Carbon dioxide + Ethanol

From a similar experiment with amylase I have seen that a change in
temperature can drastically change the rate at which yeast respires.
This is because of a theory, which is called the "collision theory"
this says that when something is heated then it will move faster thus
there will be more collisions between the substrates and the enzyme
(glucose and the yeast).

The increase in temperature increases the efficiency of the enzymes up
to a point where they are at maximum efficiency this is about 40°C;
this is called the optimum temperature. After this point the enzymes
begin to denatured, this is where they are given so much kinetic
energy what the bonds break and this leads to the active site changing
shape.

This leads onto the lock and key theory that says that the substrate
and the enzyme fit together like a lock and key and when the active
site changes shape they cannot connect and so the reaction doesn't
take place.

http://regentsprep.org[IMAGE]

The pH of the solution would alter the rate of the reaction if it was
changed therefore I must keep it constant, it will not change.

How to Cite this Page

MLA Citation:
"The Effect of Temperature on the Rate of Reaction of Yeast." 123HelpMe.com. 21 Apr 2019
    <https://www.123helpme.com/view.asp?id=120911>.

Need Writing Help?

Get feedback on grammar, clarity, concision and logic instantly.

Check your paper »

The Effect of Temperature on the Rate of Reaction Essay

- The Effect of Temperature on the Rate of Reaction Aim-We is going to investigate how temperature effects the rate of reaction when we use a Magnesium Ribbon and dissolve it in Hydrochloric Acid. Different Variables-There are four different variables that effect the rate of reaction, surface area, concentration, temperature and catalysts. Here is some information on each variable with diagrams. How does surface area effect rate of reaction. In a substance the rate of reaction will be quicker if it has a large surface area....   [tags: Papers]

Research Papers
1504 words (4.3 pages)

The Effect of Temperature on the Rate of Reaction Essay

- The Effect of Temperature on the Rate of Reaction Aim: The aim of this investigation is to find out the effect of temperature on rate of reaction I am going to investigate the changes caused to the solution of hydrochloric acid and sodium thiosulphate when the temperature is changed. Background Information: The rate of reaction varies greatly. Some chemical reactions, such as explosions, happen very quickly while others like rusting occur very slowly. The rate of reaction can be effected by a number of factors: temperature, concentration and pressuring adding a catalyst, surface area, particle size and light....   [tags: Papers]

Free Essays
898 words (2.6 pages)

The Effect of Temperature on the Rate of Reaction Essay

- The Effect of Temperature on the Rate of Reaction Aim: To investigate the effect of temperature on the speed of reaction between: Sodium thiosulphate 25ml Hydrochloric acid 5ml Sodium thiosulphate + Hydrochloric acid àSodium chloride + Sulphur dioxide + Sulphur + Water Apparatus: · Beaker · Stopwatch · Thermometer · 25ml of Sodium thiosulphate · 5ml of Hydrochloric acid · Water bath Safety: Wear goggles at all times just in case a chemical splashes into your eye....   [tags: Papers]

Free Essays
384 words (1.1 pages)

The Effect of Temperature on the Rate of Reaction Essay

- The Effect of Temperature on the Rate of Reaction Plan: We must produce a piece of coursework investigating the rates of reaction, and the effect different changes have on them. The rate of reaction is the rate of loss of a reactant or the rate of formation of a product during a chemical reaction. It is measured by dividing 1 by the time taken for the reaction to take place. There are five factors which affect the rate of a reaction, according to the collision theory of reacting particles: temperature, concentration (of solution), pressure (in gases), surface are (of solid reactants), and catalysts....   [tags: Papers]

Free Essays
970 words (2.8 pages)

The Effect of Temperature on the Rate of Reaction Essay examples

- The Effect of Temperature on the Rate of Reaction Aim The aim for this investigation is to carry out an experiment which I will be able to examine and measure the way temperature effects the rate of reaction in a number on conditions. The substances which will be used in my experiment to find how temperature effect the rate of reaction are Hydrochloric acid and Sodium thiosulphite they are going to be used at a range of different temperatures to maintain this, they are going to be placed in water baths programmed to get the substances to the right temperature before used in the experiment....   [tags: Papers]

Free Essays
1162 words (3.3 pages)

Effect Of Temperature On Rate Of Reaction Essay

- Effect Of Temperature On Rate Of Reaction PLAN: In this investigation I will find out the effect of temperature on rate of reaction. To do this I will put a piece of paper with an "X" marked on it under a chronicle flask and carry out the reaction of with Hydrochloric Acid with the concentration of 2M and Sodium Thiosulphate solution and observe how long it takes for the reaction to finish. I will repeat this with different temperatures. Apparatus: * Timer * 50g dm-3 Sodium Thiosulphate solution (Na2S2O3) * 2M Hydrochloric Acid * Conical flask * Thermometer * Bunsen burner * Heat proof mat * Wire gauze * White t...   [tags: Free Example Essays]

Free Essays
413 words (1.2 pages)

The Effect of Temperature on the Rate of Reaction Essays

- The Effect of Temperature on the Rate of Reaction Plan We must produce a piece of coursework investigating the rates of reaction, and the effect different changes have on them. The rate of reaction is the rate of loss of a reactant or the rate of formation of a product during a chemical reaction. There are five factors which affect the rate of a reaction, according to the collision theory of reacting particles: temperature, concentration (of solution), pressure (in gases), surface are (of solid reactants), and catalysts....   [tags: GCSE Chemistry Coursework Investigation]

Free Essays
2348 words (6.7 pages)

The Effect of Temperature on the Rate of a Reaction Essay

- The Effect of Temperature on the Rate of a Reaction Aim and Hypothesis The investigation that we have chosen to do is how the effect of temperature affects the rate of reaction of hydrogen peroxide to water and oxygen using the enzyme catalase. I predict that the higher the temperature the faster the rate of reaction will be and the more oxygen there will be given off....   [tags: Papers]

Free Essays
664 words (1.9 pages)

Effect of temperature on rate of reaction Essay

- Effect of temperature on rate of reaction Aim: To find out if the temperature increases the rate of reaction of two chemicals. Prediction: My prediction is that the higher the temperature is the faster the rate of the reaction will be. Equipment: Hot water, thermometer, flask, cold water, stopwatch, tissue paper, paper with ‘X’ marked on it, safety glasses, measuring cylinder, large beaker, sodium thiosulphate, hydrochloric acid. Method: The experiment has to be done five times for the five different temperatures of the sodium thiosulphate....   [tags: GCSE Chemistry Coursework Investigation]

Free Essays
428 words (1.2 pages)

Effect of Temperature on Rate of Reaction Essay

- Effect of Temperature on Rate of Reaction The enzyme amylase digests starch to form sugar.Amylase is released in the mouth and carries out the reaction at body temperature 37 degrees centigrade.I will investigate the effects of temperatures higher and lower than room temperature on the rate of this reaction. Prediction I think that the reaction will be fastest at 50 degrees centigrade ,because at body temperature reactions are carried out quickly but at a higher temperaturem the enzyme and sugar particles will move faster due to a larger amount...   [tags: Papers]

Free Essays
622 words (1.8 pages)

Related Searches

It
alters the rate of reaction because when it is acidic H+ ions
interfere with the enzymes and their active site.

Prediction

I predict that as the temperature increases, the speed of the reaction
will increase. When a particular temperature is reached I believe the
rate of reaction will dramatically decrease. I believe this because
most chemical reaction happens faster when the temperature is higher.
At higher temperatures molecules mover around faster, which makes it
easier for them to react together. Usually, a rise of 10OC will double
the rate of reaction. This is true for enzymes up to about 40°C.
However at 40°C the enzyme begins to be denatured, where the active
site changes shape and so the substrate doesn't connect with the
enzyme (lock and key), so the reaction slows down. By around 60°C the
enzyme is completely denatured. The graph below shows the trend I
believe the results will follow:

[IMAGE]


I also predict that there will be no results after the temperature has
exceeded 70°C or before 20°C. this is due to a previous experiment
which was similar where there were no results at these temperatures
with another enzyme called amylase. I think that there will be no
results after 70°C because the active site of the enzyme in the yeast
has changed shape and therefore it doesn't connect with the substrate.
And therefore didn't react. Before 20°C I think that the collision
theory comes into affect, as the enzymes do not have enough kinetic
energy to collide with enough force to cause a reaction.

Information from preliminary work.

My preliminary work helped me fine-tune my plan of the experiment by
illustrating several key faults in the experiment. The first thing I
found out was what quantities of yeast and substrate (glucose
solution) to use in the experiment. First I decided to use 1 gram of
yeast then I had several different amounts of glucose solution. I
found that less that 15ml meant that there wasn't enough sugar for the
yeast in the 6 minutes of timing. And I found that with more than 15ml
then the froth from the reaction meant that some solution pasted up
the tube. I also found that the yeast required a period of time in
order to re-activate, I found that the best length of time was 5
minutes; I got a steady stream of bubbles after this length of time.
From my preliminary work I also found that by doing more than one
repeat I could get a more accurate result and at the same time I could
eliminate anonymous results. I have also seen that there is not enough
gas at temperatures below 20°C to measure with the syringes we have
been provided. I have also seen that temperatures above 70°C do not
change the amount of gas at all, there is none being produced. I have
found that it the temperature of the water bath goes down and
therefore the temperature of the solution is going down. I am going to
have to add hot water from the water bath to keep the temperature the
same through out the time; re-activation time and timing time.

I managed to make a table of the results from the preliminary work:

Temperature

/°C

Volume of CO2 per 2 minute interval/

cm3/2 min-1

Run 1

Run 2

Run 3

Average

30

4.6

5

4.8

4.8

50

5.1

6.1

5.7

5.3

70

0

0

0

0

Range of conditions

I have chosen the range of 20°C to 70°C as I have seen from my
preliminary work that temperatures either side of this band are of no
use to the experiment.

List of variables and how they are going to be controlled

1. Independent. The only variable I am going to change is the
temperature of the water, hopefully in, 10°C intervals. By changing
the temperature of the water bath of which the boiling tube is placed
in.

2. Dependant. I will measure the volume of gas that is produced by
the reaction of the yeast and the glucose in a syringe. I will
measure it in cm3 / 2 minute interval. The syringe is placed under
water so that the CO2 pushes the water out of it and this gives us
an accurate reading of CO2 produced by the reaction. To stop any
gas escaping a Hoffman clamp is placed on the end of a rubber tube
attached to the syringe.

3. Fixed. The variables I will have to keep the same are the
concentration of substrate ([S]) and the concentration of enzymes
([E]). This is so that there is not an unfair test. If there was a
higher concentration of enzymes there would be more likeliness of
a collision and so there would be a larger amount of gas produced;
thus the mass of yeast will have to remain constant to keep it a
fair test. Similarly due to the collision theory there will be
more collisions and so the reaction will go faster, if there is
more glucose in the solution. I will also have to keep the room
temperature around the same in order to make it a fair test. The
temperature of the water bath must also stay the same or else the
temperature of the solution will decrease and so making it an
unfair test.

Number of repeats.

I will repeat my experiment 3 times at each of my 10°C intervals
(20°C, 30°C, 40°C, 50°C, 60°C and 70°C) this is so that I avoid any
anomalous results and it increases the accuracy of my experiment. The
repeats I will do will be at 2-minute intervals after a re-activation
period, 1 at 2 minutes, 4 minutes and 6 minutes. It would be even more
accurate if I could do more than 3 times but there isn't enough time
to do this.

List of apparatus

Thermometer

Water

Yeast

Glucose solution

Boiling tube

Beaker

Syringe

Delivery tube

Hoffman clip

Stirring rod

Basin

Method

Ø I measured out 1 gram of yeast and placed it in a boiling tube.

Ø I got a large beaker and filled it with water to the temperature I
wanted the glucose solution to be.

Ø I collected the glucose solution in a syringe (15ml); it had already
been heated in a water bath before hand.

Ø I filled another syringe with water in a basin of water and attached
a Hoffman clip to a rubber tube coming from the syringe, to avoid any
gas from leaving.

Ø I added the glucose to the yeast and left it to re-active, till a
steady stream of bubbles came out of the delivery tube, attached to
the boiling tube.

Ø Then I put the delivery tube so that the bubbles of CO2 went into
the syringe of water, and so pushing the water out and measuring the
amount of CO2 produce.

Ø I timed 2 minute and recorded the amount of CO2 produced. I did this
3 times.

Ø I then did it at the other temperatures and repeated the method as
above.

Ø I recorded my results in a table of results.



Safety
======

Before starting there are a few safety aspects, which I have to be
careful of. Firstly I need to be careful of the water, as some of it
is very hot.

Obtaining

A table to show the results from an experiment into the effect of
temperature on the rate of reaction of yeast.


Temperature /°C
---------------


Volume of Carbon dioxide per 2 minutes
--------------------------------------


/ cm3/2min-1
------------

1st run

2nd run

3rd run

Repeat of anomalous

average

20

2

1

2

-

1.6

30

5

4.5

5

-

4.6

40

2.5

7.5

7

7

7.2

50

5

6

6

-

5.6

60

1.5

2.3

2

1.9

2.1

70

0

0

0

-

0

All anomalous results were repeated and not used in working out the
average amount of carbon dioxide given off per 2-minute interval. The
anomalous results are highlighted in the table in red. The figures in
the average column are to 1 significant figure.

Analysis

Return to 123HelpMe.com