The Effect of Wavelength on Photosynthesis Rate
Aim:
To investigate how different wavelength (colors) of light affect the
photosynthetic rate. I will use a pant that is a pond weed called
elodea. I will measure the rate of photosynthesis by measuring the
amount of o2 given off in bubbles per minuet from the elodea. I will
do this by placing the Elodea in a test tube with sodium hydrogen
carbonate then I will vary the light wavelength (color) using colored
filters and count the number of oxygen bubbles given off using a
pencil dot technique.
Prediction
I predict that with a blue filter the photosynthetic rate will be the
highest as blue is a highly absorbent color it also has a short
wavelength so carries the most energy. The color to have the least
photosynthetic rate will be green as although it has quiet a short
wavelength so a lot of energy it will be reflected by the plant and
not absorbed. Yellow and orange will have average photosynthetic rates
although orange will be slightly more as it is more absorbent than
yellow even low it has a longer wavelength and dose not carry as much
energy as yellow would. Red will have a very high photosynthetic rate
as even though it has the longest wavelength and therefore carries the
least energy it will be greatly absorbed so a lot of the light energy
will be used rater than reflected.
Prediction graph.
[IMAGE]
Photosynthetic equation
=======================
[IMAGE][IMAGE]6CO2 + 6h20 light energy and chlorophyll C6H1206 + 6O2
Carbon dioxide + water converted into glucose and oxygen
Theory of photosynthesis
Photosynthesis is a chemical reaction, which uses the energy from
sunlight to convert carbon dioxide and water to oxygen (The bi
product) and glucose. Plants trap the energy in sunlight using
chlorophyll a light trapping pigment found in leaf plant cells. It
then uses carbon dioxide which enters the plant by small holes found
on the underside of the leaf called stoma and water which enters the
Photosynthetic pigments work by absorbing different wavelengths of light and reflecting others. These pigments are divided into two categories primary (chlorophyll) and accessory (carotenoids) pigments. Chlorophyll is then divided into three forms a, b, and c (Campbell, 1996). Chlorophyll a is the primary pigment used during photosynthesis (Campbell, 1996). This pigment is the only one that can directly participate in light reactions (Campbell, 1996). Chlorophyll a absorbs the wavelengths of 600 to 700nm (red and orange) along with 400 to 500nm (blue and violet) and reflects green wavelengths (Lewis, 2004). Chlorophyll b has only a slight difference in its structure that causes it to have a different absorption spectra (Campbell, 2004). The carotenoid involved with spinach leaf photosynthesis absorbs the wavelengths of 460 to 550nm (Lewis, 2004). The pigments are carotene and its oxidized derivative xanthophylls (Nishio, 2000). A wavelength is determined by measuring from the crest of one wave to the crest of the next wave. All the wavelengths possible are...
DPIP will be used to determine the rate at which the cholorplasts are being reduced. The spectrophotometer will establish the wavelength of light that penitrats the chloroplast solution in turn determining the amount of electrons reduced. In the dark reactions, the spectrophotometer will measure the amount of light passing through a darker solution of DPIP and chloroplasts. In the light reactions, the lighter solution, caused by reduction of the chloroplasts, will allow a larger amount of light to pass through to the photocell of the spectrophotometer. Thus, the spectrophotometer will prove wheter the light or dark reactions affect the rate of photosynthesis in chloroplasts. We will also be using a reference solution made of water, phosphate buffer, and active chloroplasts. The purpose of this solution will be used to set the transmittance level for the experiment. The control solution, which is different than the reference solution, is comprised of water, phosphate buffer, and DPIP. It will be used to prove that the three element of the solution do affect the results- it is strictly the chloroplasts that are subjected to the light/dark conditions.
at the small end of the axis to see a more reliable pattern. I could
This shows that there could be three variables in this experiment, carbon dioxide, water and light energy. So in our case the variable light energy (light intensity) will be used. The equation also shows that if there is more light energy then more glucose and oxygen will be produced.
The question we examined was how are photosynthesis and cellular respiration related? My hypothesis stated that if bromothymol blue, carbon dioxide, water and an Elodea plant are placed into test tube that is exposed to sunlight, then the bromothymol blue would change color which means that the plant has produced oxygen. When Bromothymol Blue is the color blue that indicates that oxygen is present. When Bromothymol Blue is the color green that signifies carbon dioxide is present. On Day 1, the Bromothymol Blue in all the test tubes was a orangish-yellowish-brown color. However, when we examined the Bromothymol Blue on day 2, in the control group, the color stayed same, a orangish-yellowish-brown. In the light test was bluish green and in the
The Effect of Light Intensity on the Rate of Oxygen Production in a Plant While Photosynthesis is Taking Place
Speeding up the Rate of Photosynthesis. Outline: The aim of my experiment is to find a factor that will speed. up the rate of photosynthesis.
An Analysis and Evaluation of Data from Photosynthesis Experiments Graph analysis This is my analysis for the investigation in to the affect of light intensity on the rate of photosynthesis to the Canadian pondweed, elodea. In the results the pattern is that when the light intensity is higher the readings are generally higher. On the graph the less the light intensity the lower the gradient of the curve. the equation for the photosynthesis process is; CO2 + 2H2O + Light Energy = =
Investigating the Link Between Wavelength of Light and Rate of Photosynthesis PROBLEM = = = = =
To make the test fair I will use the same amount of water and the leaf
The Effect of Light Intensity on the Rate of Photosynthesis in an Aquatic Plant Introduction The input variable I will be investigating is light, as light is just one of the 4 factors required in the green-plant process of photosynthesis. Photosynthesis is the process by which green-plants use sunlight, carbon dioxide, water & chlorophyll to produce their own food source. This process is also affected by the temperature surrounding the plant (the species of plant we experimented with, pond weed, photosynthesised best at around 20 degrees centigrade.) Light, temperature & CO2 are known as limiting factors, and each is as important as the next in photosynthesis. Light is the factor that is linked with chlorophyll, a green pigment stored in chloroplasts found in the palisade cells, in the upper layer of leaves.
* Count the number of bubbles seen in 1 minute which is a way of
An Experiment to Investigate the Effect of Light Intensity on the Rate of Photosynthesis. Introduction Photosynthetics take place in the chloroplasts of green plant cells. It can produce simple sugars using carbon dioxide and water causing the release of sugar and oxygen. The chemical equation of photosynthesis is: [ IMAGE ] 6CO 2 + 6H20 C 6 H12 O 6 + 6O2 It has been proven many times that plants need light to be able to photosynthesize, so you can say that without light the plant would neither photosynthesize nor survive.
In some way, shape, or form energy is one of the several reasons why there is an existence of life on earth. Cellular respiration and Photosynthesis form a cycle of that energy and matter to support the daily functions that allow organisms to live. Photosynthesis is often seen to be one of the most important life processes on Earth. Photosynthesis is a process by which plants use the energy of sunlight to convert carbon dioxide and water into glucose so other organisms can use it as food and energy. It changes light energy into chemical energy and releases oxygen. This way organisms can stay alive and have the energy to function. Chlorophyll is an organelle generally found in plants, it generates oxygen as a result too. As you can see without
Photosynthetic pigments are essential for life because they allow photosynthesis to occur by capturing sunlight which is then used alongside carbon dioxide and water to form organic compounds such as glucose and oxygen. The pigments allow the conversion of light energy to chemical energy which other organisms can benefit from. Oxygen is utilised by other organisms in aerobic respiration. The different pigments present in the chloroplasts allow a wide variety of wavelengths of light to be absorbed for efficient photosynthesis and provide colours to the plant to attract pollinators.