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investigating the effect of temperature on enzyme activity results
investigating the effect of temperature on enzyme activity results
investigating the effect of temperature on enzyme activity results
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The Relationship Between Time and Temperature and the Speed at which Amylase Works
Coursework
At the point 31 the time taken for the amylase to break down the
starch into glucose is 35°C seconds. As the temperature increases to
34°C, the time taken decreases to 25seconds, this is what you would
expect as the perfect temperature for the enzyme's to work is around
37°C so the nearer the temperature is to 37°C the less time will be
taken to break down starch into glucose.
At 37°C it takes the shortest time to break down the starch at 20
seconds, this is normal as this is around the body temperature at
which the enzymes work best. As the temperature is increased above
37°C the enzymes are denatured; you can tell this as the times taken
for the amylase to break down the starch starts to increase; at 40°C
the time for the starch to be broken down increases to 25seconds. When
the temperature rises again to 43°C the enzymes are clearly denatured
as it now takes 60seconds for the amylase to work effectively.
From my results I can tell that my original hypothesis; 'I predict
that as the temperature increases amylase will break starch down into
glucose faster,' is partly correct, as the temperature rises from 31°C
-34°C the time taken for the Amylase to break down the starch
decreases from 35seconds-25seconds. Also when the temperature
increases from 34°C-37°C the time also decreases from 25°C-20°C.
Although after the temperature increases above 37°C the time taken for
the amylase to break down the starch does not decrease. When the
temperature increases from 37°C-40°C the time does not decrease but
increases from 20-25seconds. Also when the temperature increases from
40°C-43°C the time again increases from
25-58seconds. This information proves my first hypothesis to be partly
correct; because as the temperature rises from 31°C-37°C my hypothesis
is proven correct as the time taken for the amylase to work decreases
from 35-20seconds showing that as the temperature increases the
amylase works faster. But above the temperature of 37°C the taken
In a substance the rate of reaction will be quicker if it has a large
The Effect of Temperature on an Enzyme's Ability to Break Down Fat Aim: To investigate the effect of temperature on an enzyme’s (lipase) ability to break down fat. Hypothesis: The graph below shows the rate increasing as the enzymes get closer to their optimum temperature (around 35 degrees Celsius) from room temperature. The enzyme particles are moving quicker because the temperature increases so more collisions and reactions occur between the enzymes and the substrate molecules. After this the graph shows the rate decreasing as the enzymes are past their optimum temperature (higher than). They are getting exposed to temperatures that are too hot and so the proteins are being destroyed.
The Effect of Temperature on the Rate of Respiration in Yeast There are two types of respiration in yeast: Aerobic: [IMAGE] Anaerobic: Glucose [IMAGE] Carbon dioxide + ethanol + energy Respiration is controlled by enzymes, which are proteins which speed up one or more biological reactions. Within any cell many chemical reactions are going on at any one time. Yeast has many different types of enzymes that speed up respiration. Prediction I predict that as temperature increases, the rate will also increase, until a certain optimum temperature, after which, the rate will decrease until the rate is zero as respiration has stopped completely. Reason
There is an optimum temperature that enzymes have for maximum productivity and its rate of reaction. This temperature is usually not that far away from the temperature of the body or room temperature. But, when the temperature is substantially reduced, like being in the ice bucket for ten minutes, this usually reduces the productivity of the enzymes. Similar to the experiment, it takes more time for the same amount of work when the temperature is severely decreased. So, an increase in temperature increases the reaction rate of enzymes. But, there is also an upper limit to the factor of temperature. After a certain temperature, the extreme heat can be harmful for the enzymes and can cause denaturation, as bonds in the enzymes can break and can change the shape of the enzyme. So, extreme low and high temperatures has a decreasing effect on the activity and reaction rate of
However, the decrease varied depending on the temperature. The lowest temperature, 4 degrees Celsius, experienced a very low decrease of amylose percentage. Temperature at 22 degrees Celsius and 37 degrees Celsius, both had a drastic decrease in amylose percentage. While the highest temperature, 70 degrees Celsius, experienced an increase of amylose percentage. In conclusion, as the temperature increases the percentage of amylose decreases; however, if the temperature gets too high the percentage of amylose will begin to increase. The percentage of amylose increases at high temperatures because there is less enzyme activity at high temperatures. However, when the temperature is lower, more enzyme activity will be present, which results in the decrease of amylose percentage. This is why there is a decrease of amylose percentage in 4, 22, and 37 degrees Celsius. In this experiment the optimal temperature is 37 degrees Celsius, this is because this is the average human body temperature. Therefore, amylase works better at temperatures it is familiar
Affect of the Rate of Reaction of Amylase on Starch and How Its Affected by the Concentration of the Substrate
Investigating Amylase Aim: The aim of the experiment is to investigate how effectively the enzyme amylase breaks down starch at different temperatures, and therefore to find the optimum temperature that amylase digests starch. Introduction: Thousands of chemical reactions take place in our cells and those reactions need to happen quickly in order to keep us active. These chemicals are called ENZYMES. Enzymes make reactions happen at a much faster rate. Enzymes come in two main types, breakers and builders.
We hypothesized that the more heat that we put in or the more heat that we take out, would denature the enzymes and slow down the rate. We set up a plate of depressions the same way as above. We boiled water to 50o C, poured the water onto a tray and did the steps of placing the discs in same as above and timed it until they rose above the surface. We did the same process but instead of using heat, we put ice and cold water on a tray which was about 3.5o C. The control for this experiment was the one that we did before because it was at room temperature. The results for the hot tray showed no rate. The cold tray sped up the rate of reactions making it occur faster than at room temperature starting at 6 for 100% catalase. This lab supported and disproved our hypotheses. It supported our hypothesis for adding more heat because the enzymes were in such hot conditions that the heat denatured the enzyme, making it not possible to create a chemical reaction. So the rate of reaction was zero because the enzymes didn’t split apart hydrogen peroxide. The cold tray disproved our hypothesis. We thought that the cold tray would also denature the the catalase so that there would be little to no rate. Maybe the data came out this way because the catalase was left out in room temperature for a long time that maybe when we took away the heat, it sped up the reaction rate.
The hypothesis is supported by the experiment in that with increased starch concentration, the amylase activity increased each time and the salivary amylase functioned best at higher concentrations of starch. Also, for the most part, the reaction followed the general trend of how at lower concentrations, the increase in reaction rate is greater, while at higher concentrations, the increase in reaction rate is less. Despite some discrepancies in trend, specifically at the 40g/L concentration, figure 1 still displays how the amylase activity eventually reaches a plateau, as mentioned in the hypothesis. From the results it appears that the point of saturation for this reaction is at the concentration 50g/L as the amylase activity rates for 50g/L
The Effect of Temperature on the Activity of the Enzyme Catalase Introduction: The catalase is added to hydrogen peroxide (H²0²), a vigorous reaction occurs and oxygen gas is evolved. This experiment investigates the effect of temperature on the rate at which the enzyme works by measuring the amount of oxygen evolved over a period of time. The experiment was carried out varying the temperature and recording the results. It was then repeated but we removed the catalase (potato) and added Lead Nitrate in its place, we again tested this experiment at two different temperatures and recorded the results. Once all the experiments were calculated, comparisons against two other groups were recorded.
The Effects of Temperature on the Action of Enzymes Aim: To see how changing the temperature affects the action of the enzyme. ------------------------------------------------------------------ MY HYPOTHESIS: I think that as the temperature increase the quicker the reaction will become ------------------------------------------------------------------- Outline of method You add ice to 150cm³ of cold water in a beaker to bring its temperature down to 10cº. Then a fixed volume of hydrogen peroxide is added to a pre-weighed piece of liver contained in a test tube. Then the maximum height reached by the foam produce was tabulated.
The Effect of Temperature on the Rate of Respiration in Yeast I have chosen to investigate the affect temperature has on the rate of respiration in yeast. I will use an experiment to determine whether the yeast's rate of respiration will be quicker, slower or if it does not change when the temperature is varied. Scientific Knowledge The first thing to say about enzymes is that they are proteins and they are found in all types of organisms from humans to viruses.
Experiment to Demonstrate the Action of Amylase on Starch Solution. Plan = == =
How Amylase Concentration Affects the Rate of the Starch Concentration In this piece of coursework, I have to carry out an investigation to find out how amylase concentration affects the rate of starch. Enzymes are biological catalysts that speed up the chemical reaction that goes on inside living things. An enzyme acts on substrate and may do its job inside or outside the cells. However, the rate at which enzymes work are affected by the following factors/variables: Concentration:
The pH of the solution would alter the rate of the reaction if it was