Western blot has been a revolutionary technique for identifying the expression of proteins within relative molecular biological samples that shared the same ancestor. Moreover, the sensitivity and specificity of the western blot (Immunoblotting) enables it a common technique for determining specific protein levels in clinical samples. Since the antibody specific to the antigen immunospecificity), it enables the target protein to be identified. Western blotting can produce quantitative data about that protein, which in this case the difference between bands in each of the protein samples. The western blot is an analytical technique used to detect specific proteins in the given sample of tissue homogenate or extract. The proteins are then transferred to a membrane (in this case, nitrocellulose), where they are stained with antibodies specific to the target protein  .
This experiment designed to analyze the phylogenetic of four unknown different fish muscles samples in order to predict the type of the aquatic creature. In this study, actin and myosin proteins were analyzed on account of their fairly high and constant expression in the animal muscles. Since the actin and myosin proteins are the two major...
... middle of paper ...
...and arthropod (shrimp/krill). Well number 7 has low protein actin and myosin and can be identified as shrimp, and wells 4, 5, 6 are identified as salmon, tilapia, and cod, respectively .
In figure 2, it is clear that the protein was transferred successfully from the gel to the membrane. The blot analysis was performed to detect if the protein was expressed.
The amount of protein present in each sample dilution in the blot corresponds with an absorbance value for that unknown protein extract. The antibody will bind to the protein (actin or myosin) and the Horse Raddish Peroxidase will catalyze the cleaving of the substrate to produce color upon antibody-protein binding. According to figure 3, 4, 5, 6 lanes are similar which confirm that they shared the same ancestor. However, lane 7 is slightly different which approves again it evolves from different ancestor.
Need Writing Help?
Get feedback on grammar, clarity, concision and logic instantly.Check your paper »
- ... By identifying these scents when returning to the nest, they will be able to better locate it. This study found that birds have preferences for the scent of their mates, which promotes the investigation of how olfactory cues can be used in the selection of appropriate mates. One of the most important aspects of mate selection involves the identification of kin. When breeding, birds often stay close to their colony of nests. By doing so, they avoid the risk of encountering predictors in a new site.... [tags: chemical signals in birds]
1750 words (5 pages)
- In today’s society everyone seems to have an opinion on everything. It seems the more uninformed one is on a subject the more strongly the feel about it. Occasionally there are those who address issues they are well informed on in a well-organized and civilized manner, weighing both the pros and cons of the side they advocate. Brandon M. Middleton does an exceptional job of this in his article on the Endangered Species Act, where he talks about the effectiveness of the Federal government’s current involvement with endangered species.... [tags: Endangered species, Species]
1423 words (4.1 pages)
- 1. Privet plant and different species of the Asian carp fish depict two instances when non-native species which were introduced in Tennessee causing problems. Privet is a flowering plant native to Europe, Asia and North Africa (“Non-native Invasive Plants”). It has been introduced to grow as a hedge [boundary]. But, this plant once planted can take over the native plants and grow in dense groups. Currently, the Great Smoky National Park crews work up to “550 work hours each year” to remove privet plants (“Non-native Invasive Plants”).... [tags: Invasive species, Introduced species, Carp]
735 words (2.1 pages)
- Ampelopsis brevipedunculata A Connecticut Invasive Species Throughout Connecticut, invasive plant species are taking over the unkempt spaces. One particular plant, Ampelopsis brevipedunculata, is one of these species. A. brevipedunculata goes by many names across the world; most common amongst them porcelainberry or peppervine. Like most invasive species, it was brought here as an “exotic” ornamental, prized for its colorful autumn berries. In 1870 A. brevipedunculata, was introduced into the United States from its native region of the temperate areas of Asia, most often in Japan and China.... [tags: Invasive species, Introduced species, Weed]
1394 words (4 pages)
- Darwinism is a theory developed by Charles Darwin with the help of many others. It states that natural selection is the most common cause of evolution. All species of organisms arise and grow through the natural selection of inherited variations that help increase the individual's ability to survive and reproduce in its environment. 1735 Carolus Linnaeus: He believed that God had created the world in a divine order from the simplest creatures up to human beings. He said God created struggle and competition to maintain the balance of nature.... [tags: evolution, species, organisms, environment]
984 words (2.8 pages)
- Foreign species from a far away land invading American soil and causing great amounts of destruction. Sounds like something out of a science fiction movie right. Wrong. Invasive species exist in the world and are a very real threat to the American citizens ' way of life. The nations culture, resources, economic growth, waterways, forest’s s and even as personal as citizens backyard are all at risk of being damaged beyond repair. Invasive species affect the nations environment by causing major environmental damage, spreading disease, proliferating at high rates, consuming a vast quantity of the key resources needed for trade and other essential aspects of society.... [tags: Invasive species, Introduced species]
1587 words (4.5 pages)
- The Relational Nature of Species Concepts ABSTRACT: Édouard Le Roy as early as 1901 observed the existence of an intellectual movement seeking to break from traditional positivism and set for himself the task of drawing up the program of this new positivism. Noting that this program precedes the Vienna Circle, I endeavor to determine its nature and to evaluate its impact on logical positivism. Viewed in this light, the discussions between Le Roy, Poincaré and Duhem appear more prolonged and substantial than is usually thought.... [tags: Species Positivism Essays]
3452 words (9.9 pages)
- Have you ever wondered how many plant and animal species are threatened by extinction. In an article of an issue of the Discovery Education magazine it stated “To date 16,928 plant and animal species are believed to be threatened with extinction.” (Extinction Facts) That is an enormous number and with the constant growing and changing world that number will only increase. Based on all the environmental factors placed on animals, the extinction of untamed animals is inevitable but, steps should be taken to preserve the remains of untamed animals so that scientific research can be conducted.... [tags: Extinction, Endangered species, Global warming]
873 words (2.5 pages)
- For example, zoos are good for the protection of endangered species. According to Dr. Dave Hone in his article “Why Zoos are Good” he states, “Zoos protect against a species going extinct. A species protected in captivity provides a reservoir population against a population crash or extinction in the wild” (Hone). Hone is stating the claim that zoos are relatively safe and can provide a place for the species to breed to provide a foundation population. This will allow a nearly extinct species to slowly bounce back and survive for future generations.... [tags: Extinction, Biodiversity, Endangered species]
1187 words (3.4 pages)
- The Identify The problem The End User The Company is called Vesco and has 13 stores round the UK. The manager from one of their London stores has asked me to do the following for his supermarket only. The Problem A company called food on the go are their home delivery company. The manager of Vesco has asked me to make invoices for the “food on the go” company and their customers. The manager of Vesco called Dean Smith said that their old way of billing people was very slow. What they did was they would send an employee around the supermarket to get each item that has been ordered and would have to add up the prices at the end.... [tags: Business and Management Studies]
2544 words (7.3 pages)