During my freshman year at the University of Toledo, I joined Dr. Jared Anderson 's bioanalytical chemistry lab, with the research topics regarding the use of magnetic ionic liquids (MILs) as solvents for rapid DNA extraction and preconcentration from aqueous samples for further downstream experiments. Most...
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...cterium. I obtained and refined a 2.1 Å crystal structure of citrate synthase, encoded by CitA, containing the desired electron density for incubated pyruvate, and ran differential scanning calorimetry and isothermal titration calorimetry experiments to quantitatively-examine the key binding interactions. Furthermore, I am working to test the hypothesis that the reactive thiol functional group at the −3 position of a protein is undergoing fatty acid modification in mycobacteria, a common occurrence among eukaryotes for protein translocation to the plasma membrane. In this realm, a protein was engineered to contain a green fluorescence protein tag followed by a poly-histidine tag, a PreScission Protease cut site, and a cysteine-valine-asparagine C-terminal domain. Upon expression and purification of the protein, ESI-MS will be used as a tool to determine modification.
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Statement of Purpose as a Researcher in the Field of Power Systems at Michigan Technological University
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