Thin Layer Chromatography I. Abstract The purpose of this lab was to determine the rate of flow values (Rf=distance of component/distance of solvent) for certain pigments found in chlorophyll and carotene. For the chlorophyll, we observed the following five colors: yellow, orange, dark green, green, and light green. For the cartenoid, we observed the following five colors: yellow, auburn, purple, pink, and red. The solvent in both of our experiments traveled a distance of 6.8cm. We obtained
Introduction Thin layer chromatography is a classical case of adsorption or solid/liquid chromatography or planar chromatography. In planar chromatography, the stationary phase is applied on a flat surface and movement of mobile phase is due to the capillary action. The stationary phase is normally a polar absorbent and the mobile phase can be a single solvent or combination of solvents. Adsorption is a concentration dependent process and adsorption coefficient is not constant, in contrast to partition
This lab used thin-layer chromatography to analyze the polarity of an Analtech dye mixture and the polarity of pigments isolated from a spinach leaf using liquid-liquid extraction. Chromatography is a method of separating mixtures into their pure compounds. The separation occurs because the intermolecular attractions in the mixture differ in their polarity. The stationary phase also has intermolecular attractions. Separation occurs as the mobile phase passes over the stationary phase. All types of
Hello student! In order to solve your problem, it is important to first understand how thin layer chromatography works. Thin Layer Chromatography is a way in which we can separate components of mixtures and identify qualities about their chemical nature. The TLC plate is made of a silica gel which plays an integral role in the distance the different samples will travel, which we will discuss later. For now, it is important to know that Silica gel is very polar. The solvent, which is what you are
In this lab, Thin Layer Chromatography was used to identify the components of a certain drug. To do this, the compound in question, Motrin was tested against six standards in three different solvents. The three solvent were hexanes, ethyl acetate, and 75% ethyl acetate and 25% hexane; the solution of 75% ethyl acetate and 25% hexane was determined to be the best solvent. This is due to the larger variance in RF values. The six standards that Motrin was tested against were Aspirin, ibuprofen, acetaminophen
The Thin layer chromatography is one of the oldest techniques which is used to identify what is present in an unknown mixture. It is a very useful technique for research,forensics,environmental testing, and many more fields because it is simple and inexpensive way to analyze small samples. In this experiment two compounds (solid) were used ferrocene and acetylferrocene. In the first step 50 ml of 2:8 diethyl ether/petroleum ether was placed in an erlenmeyer flask covered with a parafilm paper to
The purpose of these lab was to help students understand the chromatographic techniques of column chromatography and Thin layer chromatography. Column chromatography is used to help students understand the relationship between eluting power and polarity. Eluting power is defined as the ability of the mobile phase to move a substance from stationary phase. The polarity of the solvent used in the lab can be described as Methanol> acetonitrile>acetone>ethyl acetate> hexane in decreasing order. Since
What is chromatography along with how is chromatography used in Forensics? Well in this research paper I will be answering these questions, while explaining how chromatography has been used to solve cases not only in Georgia, but throughout the United States. First, I will start by explaining that chromatography is the separation of a mixture by passing it in a solution or suspension or as a vapor (as in gas chromatography) through a medium in which the components move at different rates. There is
Chromatography is the laboratory technique for separating mixtures into their components for analyzing, identifying, purifying or quantifying the mixtures or components. The mixture dissolved in the mobile phase (gas or liquid) is passed through the stationary phase (liquid or solid), which separates the analyte from the other molecules in the mixture. The differences in the migration rate of the compounds in these two phases effects the separation. Chromatography is either preparative or analytical
the method of column chromatography, and then determine the purity of the isolated compounds through thin-layer chromatography (TLC), as well as melting point analysis. Through column chromatography, gravity is utilized to move the solvent and compounds down the column, where the changes in the solvent polarity assists in eluting the desired compounds to separate fractions. Each fraction solvents can then be evaporated to obtain the compounds of interest. Through TLC, a thin layer of polar and hydrophilic
chiral secondary alcohol using the competing enantioselective conversion (CEC) method. This method uses both R- and S- enantiomers of a chiral acyl-transfer catalyst called homobenzotetramisole (HBTM), in separate parallel reactions, and thin layer chromatography to identify the stereochemistry of the secondary alcohol, whether it be an R- or S- enantiomer. Quantitative analysis was performed using a program called ImageJ after the appropriate picture was taken of the stained TLC plate. The molecular
Identifying an Unknown Analgesic Objectives: 1. To identify a unknown analgesic 2. To identify the analgesic you will be given 4 known analgesics compounds. Each of which can be separated using by Thin Layer Chromatography. 3. Using thin layer chromatography the unknown analgesic will be compared to the 4 known analgesics. Spotting of the sample 1. Prepare a microcapillary tube for each analgesic and each standard 2. Immerse the small end of the tube into the sample vial
http://www.chymist.com/Chromatography%20food%20colors.pdf Chemistry Lab 016b , Science In Motion, University of Pittsburgh at Bradford http://www.trinity.edu/fwalmsle/MandMFiles/Structures%20of%20Some%20Food%20Dyes.doc Determination of Food Dye Concentrations in an Unknown Aqueous Sample Using HPLChttp://www.wbu.edu/academics/academic_resources/writing_center/resources/documents/ScienceSampleUndergraduatePaper1-Determinationof....pdf CHEM 1011, Separation of Dyes by Paper Chromatography, Austin Peay State
Introduction Chromatography is an extensive range of laboratory technique which aims to separate complex mixtures into their components which are distributed between a stationary phase bed and a mobile phase. There are various methods of chromatography. One method of mixture separation by chromatography is the column chromatography. Column chromatography gives a clear visual separation of components throughout the column. It is a cheap, simple method however it is time consuming. Another method
ENTHALPY What is enthalpy? Enthalpy is the amount of heat content used or released in a system at persistent pressure. Enthalpy is typically articulated as the change in enthalpy. The change in enthalpy is linked to a change in internal energy (U) and a change in the volume (V), which is multiplied by the constant pressure of the system. 23/04/14 (http://chemwiki.ucdavis.edu/Physical_Chemistry/Thermodynamics/State_Functions/Enthalpy) When the reaction is giving off heat, it is exothermic. In this
Introduction: Chromatography is a technique used to separate small quantities of mixtures into its individual components. This is done by distributing the components between two phases: stationary and mobile. The stationary phase is the system in which the materials to be separated are absorbed. The mobile phase is the mixture of solvents that flows through the stationary phase. Separation for each substance is based on the different affinities each has for each phase, low affinity for moving phase
Extracting Lipids from Ground Nutmeg I. Introduction In this experiment, lipids from ground nutmeg are extracted using a combination of solvents and identify the lipids through chromatography. The purpose of using solvent combinations is to elute the lipids based on their polarity to binding of the silica gel. The chromatography is performed on a silica gel plate and the use of iodine to visualize the lipids. By calculating the Rf values for each compound and comparing them to the known lipids, we are
Separation of Photosynthetic Pigments by Paper Chromatography Introduction Chlorophyll is in fact only one pigment in a group of closely related pigments commonly found in photosynthesising plants called photosynthetic pigments. This can be demonstrated by extracting the pigments from leaves with acetone and separating them by means of paper chromatography. With a bit of luck five pigments can be identified: chlorophyll a (blue-green), chlorophyll b (yellow-green), xanthophylls (yellow)
purple color of the starting H2TTP. Introduction Thin layer chromatography, or TLC, is used as a prerequisite for column chromatography. When performing types of chromatography, like TLC, the polarity of solvents is extremely important. This lab uses TLC plates, five solvents (hexane, toluene, ethyl acetate, dichloromethane, and acetone), and small evaporating dishes to determine which solvent would be best to use in column chromatography. The least polar solvent is hexane, which has a polarity
Synthesis and Characterization of Aspirin Product Identity and Purity After synthesizing a chemical, especially a drug, it is important to confirm the identity and purity of the product. You will perform three tests to examine the identity and purity of the aspirin that you synthesized. One test will detect the presence of leftover salicylic acid in the synthesized aspirin and allow you to determine its concentration. Government regulations stipulate that commercial aspirin must not contain residual